Method for inducing umbilical cord mesenchymal stem cells to be differentiated into leydig cells through gold nanoparticle medicated gene transfection

A technology of Leydig cells and nano-gold particles, which is applied in the fields of cell biology and developmental biology, can solve problems such as undifferentiated Leydig cells, and achieve the effect of improving application prospects

Inactive Publication Date: 2015-11-18
GUANGZHOU STEMOVE BIOTECH CO LTD
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  • Abstract
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  • Claims
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Problems solved by technology

After investigation, there is no report on the method of inducing human umbilical cord mesenchymal stem cells to differentiate into Leydig cells through gene transfection mediated by gold nanoparticles

Method used

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Embodiment Construction

[0022] The technical solutions of the present invention will be described in detail below through specific examples, but the scope of the present invention is not limited by these examples.

[0023] A method for inducing human umbilical cord mesenchymal stem cells to differentiate testicular Leydig cells through gold nanoparticle-mediated gene transfection, comprising the following steps:

[0024] 3) Synthesis of gold nanoparticles, coating of gene carriers by gold nanoparticles, and gene transfection mediated by gold nanoparticles;

[0025] 4) Using compounds or growth factors to induce human umbilical cord mesenchymal stem cells to differentiate into Leydig cells

[0026] The synthetic process of described nano gold particle is as follows:

[0027] (1) Chlorauric acid (HAuC1 4 ) into a 0.01% aqueous solution, take 100mL and heat to boiling.

[0028] (2) Accurately add 1.5 mL of 1% trisodium citrate (Na3C6H5O7·2H2O) aqueous solution under stirring.

[0029] (3) Continue h...

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Abstract

The invention provides a method for inducing umbilical cord mesenchymal stem cells to be differentiated into leydig cells through gold nanoparticle medicated gene transfection. Plasmids with SF-1 (seroidogenic factor 1) genes and LHR (luteotropic hormone receptor) genes are cotransfected into the umbilical cord mesenchymal stem cells through gold nanoparticles, then the umbilical cord mesenchymal stem cells are induced by compounds or growth factors to be directionally differentiated into the leydig cells. The leydig cell differentiation method is efficient and safe, the differentiated leydig cells can serve as a research substitution model and can be used as seed cells of clinical cell treatment of the leydig cells, and an application prospect of leydig cell regeneration replacement treatment is widened.

Description

technical field [0001] The invention belongs to the fields of cell biology and developmental biology, and specifically relates to a method for inducing human umbilical cord mesenchymal stem cells to differentiate testicular mesenchymal cells through gene transfection mediated by nano gold particles. Background technique [0002] Andropause syndrome is a series of symptoms caused by the atrophy of the testes, the decrease of the secretion of testosterone, the decrease of the response of the atrophic testes to gonadotropins, and the imbalance of the regulation function of sex hormones in the body. The main manifestations are mental and psychological symptoms: inability to concentrate, memory loss, depression, anxiety, irritability, suspicious, nervousness, and decreased work ability. Vasomotor symptoms are palpitations, hot flashes, and sweating. Sexual symptoms include decreased interest in sex, decreased libido, and impotence. Physiological and physical symptoms include de...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/10C12N5/077
Inventor 冯文峰黄鹏昌芒
Owner GUANGZHOU STEMOVE BIOTECH CO LTD
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