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Method for induced differentiation of testicular interstitial cells and applications of testicular interstitial cells in sexual function recovery

A technology of Leydig cells and induced differentiation, applied in a method and its application field in the restoration of sexual function, can solve the problems of large differences in serum testosterone levels, inhibition of secretion and spermatogenic functions, and disruption of the natural rhythm of androgen, etc. problems, to achieve the effect of easy preparation and use, restoration of male sexual function, and strong practicability

Inactive Publication Date: 2018-09-28
深圳市莱利赛生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, long-term use of androgen has serious side effects. First, there are many target cells of androgen in the body, and long-term use of exogenous androgen therapy is likely to cause toxic and side effects, especially in the prostate; second, the differences in serum testosterone levels among different individuals Third, testosterone replacement therapy will destroy the natural rhythm of androgen secretion in the human body, which is high in the morning and low in the evening, and will also inhibit the androgen secretion and spermatogenic function of the testes

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] A method for inducing differentiation of Leydig cells, comprising the following steps:

[0027] (1) Cultivation of mesenchymal stem cells: Mesenchymal stem cell monomers were divided into 4×10 5 / ml inoculated in the stem cell culture medium, placed in a 38°C incubator, lowered to 36°C at a rate of 1°C / day, and then kept at a constant temperature of 36°C, and then changed the medium every 2 days for passage, and harvested 4 Substitute mesenchymal stem cells, then add 0.3% collagenase and 0.25% trypsin with a volume ratio of 2:1.5 successively to digest for 25 minutes, and then vibrate with an oscillator for 10 minutes to obtain cultured and expanded mesenchymal stem cells; wherein the stem cell culture The solution is DMEM and F12 mixed at a volume ratio of 1.5:1 and 3% FBS is added as the basic culture solution, which also contains 55ng / ml EGF, 15ng / ml bFGF, 3% non-essential amino acids, 0.3mM GABA, 1.5% multivitamins, The balance is ultra-pure water. FBS contains the...

Embodiment 2

[0042] A method for inducing differentiation of Leydig cells, comprising the following steps:

[0043] (1) Cultivation of mesenchymal stem cells: Mesenchymal stem cell monomers were divided into 5×10 5 / ml inoculated in the stem cell culture medium, placed in a 38°C incubator, lowered to 36°C at a rate of 1°C / day, and then kept at a constant temperature of 36°C, and then changed the medium every 3 days for passage, and harvested 5 Substitute mesenchymal stem cells, then add 0.3% collagenase and 0.25% trypsin with a volume ratio of 2:1.5 successively to digest for 32 minutes, and then vibrate for 11 minutes by an oscillator to obtain cultured and expanded mesenchymal stem cells; wherein the stem cells were cultured The solution is DMEM and F12 mixed at a volume ratio of 1.5:1 and 3% FBS is added as the basic culture solution, which also contains 55ng / ml EGF, 15ng / ml bFGF, 3% non-essential amino acids, 0.3mM GABA, 1.5% multivitamins, The balance is ultra-pure water. FBS contain...

Embodiment 3

[0058] A method for inducing differentiation of Leydig cells, comprising the following steps:

[0059] (1) Cultivation of mesenchymal stem cells: Mesenchymal stem cell monomers were divided into 6×10 5 / ml inoculated in the stem cell culture medium, placed in a 38°C incubator, lowered to 36°C at a rate of 1°C / day, and then kept at a constant temperature of 36°C, and then changed the medium every 3 days for passage, and harvested 6 Substitute mesenchymal stem cells, then add 0.3% collagenase and 0.25% trypsin with a volume ratio of 2:1.5 successively to digest for 40 minutes, and then vibrate with an oscillator for 12 minutes to obtain cultured and expanded mesenchymal stem cells; wherein the stem cell culture The solution is DMEM and F12 mixed at a volume ratio of 1.5:1 and 3% FBS is added as the basic culture solution, which also contains 55ng / ml EGF, 15ng / ml bFGF, 3% non-essential amino acids, 0.3mM GABA, 1.5% multivitamins, The balance is ultra-pure water. FBS contains the...

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PUM

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Abstract

The invention discloses a method for induced differentiation of testicular interstitial cells and applications of the testicular interstitial cells in sexual function recovery, belonging to the technical field of biological medicines. Mesenchymal stem cells are utilized for induced differentiation of testicular interstitial cells, the testicular interstitial cells and other ingredients with a certain ratio form a medicine for sexual function recovery, the medicine for sexual function recovery is prepared into an injection, the injection is injected to the focus area in the injection form, thetestosterone level in the body is recovered in a manner that the testicular interstitial cells secrete testosterone, and thus the sexual function of the male is effectively recovered. In short, the method is safe and effective, and is strong in practicability, testicular interstitial cells are efficiently obtained through induced differentiation, and the medicine can effectively recover the sexualfunction of the male.

Description

technical field [0001] The invention relates to the technical field of biomedicine, in particular to a method for inducing and differentiating Leydig cells and its application in sexual function recovery. Background technique [0002] Mesenchymal stem cells (MSCs) are adult stem cells with self-replication ability and multi-directional differentiation potential, which can develop into bone, cartilage, fat and other types of cells. Mesenchymal stem cells can be transplanted, and the type of cells they grow into depends on where they are injected. For example, mesenchymal stem cells injected into the heart can form healthy new tissue, among other things. In 1976, Freidenstein discovered for the first time that there was a group of impure cell populations in the bone marrow. When cultured in vitro, these cells adhered to the wall and grew similarly to fibroblasts. The concept of mesenchymal stem cells, MSC). After in-depth and extensive research, it was found that it exists i...

Claims

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Application Information

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IPC IPC(8): C12N5/071A61K35/52A61P5/26
CPCA61K35/52A61P5/26C12N5/0683C12N2500/32C12N2500/33C12N2500/38C12N2500/40C12N2501/11C12N2501/115C12N2501/998C12N2506/1392
Inventor 宋芸娟胡士庶张治国
Owner 深圳市莱利赛生物科技有限公司
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