Construction and application of model for differentiating stem cells into testicular interstitial cells

A technology of Leydig cells and embryonic stem cells, which is applied in the fields of developmental biology and pharmacology, can solve problems such as the screening of unseen pro-cell differentiation agents, and achieve the effect of improving application prospects
CN101892190AInactive Publication Date: 2010-11-24ZHEJIANG UNIV
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
ZHEJIANG UNIV
Publication Date
2010-11-24
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention provides a method for constructing a model of oriented differentiation of embryonic stem (ES) cells into testicular interstitial cells. The method comprises the following steps of: firstly, cotransfecting a steroidogenic factor 1 and a luteinizing hormone receptor plasmid into an ES cell; then inducing the ES cell by a compound or growth factor to directionally differentiate the ES cell into the testicular interstitial cell. In the invention, the construction and the application of the model for in-vitro oriented differentiation of the stem cells into the testicular interstitialcells are demonstrated at the cellular level; a great deal of biology information is discussed initially; the constructed cell model is a substituted model for researching the influencing factor of the secretory regulation function of the testicular interstitial cell and can be used for initially selecting and evaluating the medical effect of the pharmaceutical drug using the testicular interstitial cell as the target cells; the transplanting of the testicular interstitial cell differentiated from the stem cells is used as a new method for supplying testosterone, and the application prospect of the renewable alternative treatment of the testicular interstitial cell is improved.
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Description

technical field

[0001] The invention belongs to the field of developmental biology and pharmacology, and relates to the construction and application of a model, in particular to the construction of a model of stem cells directed differentiation into Leydig cells in vitro, and its induction with the aim of regulating the secretory function of Leydig cells Differentiation agent screening application. Using the transplantation of Leydig cells differentiated from stem cells as a new method of supplementing testosterone will improve the application prospect of Leydig cell regeneration and transplantation. Background technique

[0002] Andropause syndrome, that is, partial androgen deficiency in middle-aged and elderly men (PADAM) or hypogonadism in middle-aged and elderly men is a social problem of general concern at present. At present, chemically synthesized androgen is mainly used in clinic for replacement therapy. However, the long-term application of chemically synthesized...

Claims

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