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Method for detecting trace luteinizing hormone

A technology for luteinizing hormone and a detection method, which is applied in the field of trace luteinizing hormone detection, can solve the problems of low luteinizing hormone concentration and low accuracy of measurement results, and achieves the effect of improving the sensitivity of the method

Active Publication Date: 2011-01-05
高常青 +2
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  • Summary
  • Abstract
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AI Technical Summary

Problems solved by technology

These methods are direct or indirect detection of luteinizing hormone itself, but due to the low concentration of luteinizing hormone in the actual sample, the accuracy of the determination results is also low

Method used

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  • Method for detecting trace luteinizing hormone

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0017] Embodiment: This experiment takes guinea pig Leydig cell bioassay as an example:

[0018] (1) Preparation of LH-free serum:

[0019] A 0.25cm (inner diameter 0.062 inch, outer diameter 0.095 inch) silicone rubber tube was filled with estradiol crystals (Sigma, St. Louis, Missouri, USA), and subcutaneously implanted on the back of guinea pigs for 4-6 weeks to inhibit the production of LH in animals. Secretion; collect 50 ml of serum, add 10-20 mg of activated carbon, absorb at room temperature for 12 hours, filter with filter paper to remove steroid hormones, and store the filtrate in a -30°C refrigerator;

[0020] (2) Preparation of guinea pig testis Leydig cells:

[0021] Take 2 guinea pig testicles with a body weight of 550-700g and remove the capsule; add collagenase III and dissociative enzyme II to 199 culture medium (Sigma Company, St. Louis, Missouri, USA, 6.2ml / 2 testes) to make the culture medium The final concentration of collagenase III in the medium was 0....

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PUM

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Abstract

The invention discloses a method for detecting trace luteinizing hormone. The method comprises the following steps: (1) preparation of luteinizing hormone free serum; (2) preparation of testicular interstitial cell; (3) analysis on luteinizing hormone in a serum sample of a detected person; and (4) back calculation of a detection result of testosterone on a luteinizing hormone standard curve, andcheck on concentration of luteinizing hormone in the sample to be detected. The method can detect LH with extremely low concentration, has greatly improved sensitivity, is 100 times more sensitive than the existing testicular cell radio-immunity method, and is 1,000 times more sensitive than the chemiluminesence immunoassay.

Description

technical field [0001] The invention relates to a method for detecting a trace amount of luteinizing hormone. Background technique [0002] Luteinizing hormone (LH) is a protein hormone secreted by the cells of the anterior pituitary gland. LH in men stimulates testicular cells to produce testosterone, and LH in women stimulates ovulation and maintains the corpus luteum. The corpus luteum secretes progesterone. At present, the detection of luteinizing hormone is mainly through enzyme-linked immunoassay, radioimmunoassay and chemiluminescence. These methods all directly or indirectly detect luteinizing hormone itself, but due to the low concentration of luteinizing hormone in the actual sample, the accuracy of the determination results is also low. Therefore, it is necessary to establish a more sensitive detection method. Contents of the invention [0003] The object of the present invention is to provide another detection method of trace luteinizing hormone. In order ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/74G01N1/28
Inventor 高常青高永晴田韵
Owner 高常青
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