Composition for improving production performance of hens and application thereof

Abstract

The invention discloses a composition for improving the production performance of hens and application thereof, and the composition for improving the production performance of hens comprises the following components in parts by mass: 5-9 parts of a motherwort-caulis spatholobi composite extract and 1-3 parts of a quamoclit vine extract. The herba leonuri, the caulis spatholobi and the quamoclit climoclit are compounded for the first time, the herba leonuri prefers on reproductive health conditioning, the caulis spatholobi prefers on blood replenishing and oxidation resistance, the quamoclit climoclit prefers on antibacterial and anti-inflammatory effects, the advantages of the herba leonuri, the caulis spatholobi and the quamoclit climoclit are superposed, the reproductive function and overall health of hens are improved in a targeted mode, and the defect of a single traditional Chinese medicine on the broad-spectrum function is overcome.

Description

Technical Field

[0001] The present invention relates to the technical field of hen breeding, and particularly relates to a composition for improving the production performance of hens and its application. Background Art

[0002] In recent years, with the increasing global attention to food safety and environmental sustainability, the livestock industry is gradually transforming towards a non-antibiotic and green breeding model. "Banning antibiotics at the feed end and reducing antibiotics at the breeding end" has become an industry consensus, aiming to reduce the use of antibiotics in the animal breeding process, thereby reducing antibiotic residues in the food chain, reducing environmental pollution, and reducing the generation of drug-resistant strains. The key to implementing this strategy lies in finding and developing natural, safe, and environmentally friendly antibiotic alternatives. Among them, plant-derived active substances and acidifiers have become the key research and application directions in the current livestock industry due to their antibacterial, immune-regulating, intestinal health-improving, and production performance-promoting effects.

[0003] Plant extracts, due to their natural bioactive components, can replace the role of antibiotics at different levels, including antibacterial, anti-inflammatory, antioxidant, immune regulation, and promoting nutrient absorption. Among them, many Chinese herbal medicines have been applied to a certain extent in the breeding industry due to their special pharmacological effects. For example, Astragalus membranaceus, Glycyrrhiza uralensis, Lonicera japonica, Echinacea purpurea, etc. are widely used in livestock and poultry breeding due to their immune-regulating and disease-resistant effects to improve animal health and reduce the dependence on antibiotics. Leonurus japonicus is rich in alkaloids, flavonoids, and polysaccharides, which can promote uterine health, regulate the secretion of reproductive hormones, and improve the reproductive health of female livestock and poultry. Spatholobus suberectus is rich in phytosterols, flavonoids, and polyphenolic compounds, which can promote blood formation in animal bodies, improve the body's immunity and disease resistance. In the breeding field, extracts of Spatholobus suberectus have been studied for improving anemia, enhancing the body's antioxidant capacity, and improving production performance. Ipomoea quamoclit has strong antibacterial and anti-inflammatory effects, and can effectively reduce the inflammatory response of animal bodies, especially showing good application potential in respiratory and digestive tract diseases. Although there have been certain studies on the single application of Leonurus japonicus, Spatholobus suberectus, and Ipomoea quamoclit in livestock and poultry breeding, there is no systematic research and application on the compounding of the three to improve the production performance of hens in the existing technology. Summary of the Invention

[0004] In view of the above-mentioned prior art, the object of the present invention is to provide a composition for improving the production performance of hens and its application.

[0005] To achieve the above object, the present invention adopts the following technical solutions:

[0006] In the first aspect of the present invention, there is provided a composition for improving the production performance of hens, comprising the following components in parts by mass: 5-9 parts of Leonurus japonicus-Spatholobus suberectus compound extract and 1-3 parts of Ipomoea quamoclit extract.

[0007] Preferably, the Leonurus japonicus - Spatholobus suberectus composite extract is prepared by the following method:

[0008] Crush Leonurus japonicus and Spatholobus suberectus according to a mass ratio of (4 - 6):(1 - 3) to obtain a mixture. Mix the mixture and an ethanol solution with a volume fraction of 80% - 90% evenly according to a mass ratio of 1:(10 - 15), perform ultrasonic extraction 2 - 4 times, filter, combine the extracts, concentrate, and dry to obtain the Leonurus japonicus - Spatholobus suberectus composite extract.

[0009] Preferably, the time for ultrasonic extraction is 15 - 20 min, and the ultrasonic power is 150 - 250 W.

[0010] Preferably, the Quamoclit pennata extract is prepared by the following method:

[0011] (1) Dry and crush the whole herb of Quamoclit pennata to obtain Quamoclit pennata powder;

[0012] (2) Add distilled water to the Quamoclit pennata powder, extract at 90 - 100 °C for 1 - 2 h, filter, and cool the filtrate to obtain an aqueous extract;

[0013] (3) Add Bacillus subtilis seed liquid and Lactobacillus reuteri seed liquid to the aqueous extract for fermentation to obtain a fermentation broth;

[0014] (4) Sterilize, concentrate, and dry the fermentation broth to obtain the Quamoclit pennata extract.

[0015] Preferably, in step (2), the mass ratio of Quamoclit pennata powder to distilled water is 1:(10 - 20).

[0016] Preferably, in step (3), the inoculation amount of Bacillus subtilis seed liquid is 3 - 5% of the volume of the aqueous extract, and the inoculation amount of Lactobacillus reuteri seed liquid is 3 - 5% of the volume of the aqueous extract.

[0017] Preferably, in step (3), the viable count of Bacillus subtilis seed liquid ≥ 1×10 8 cfu / ml; the viable count of Lactobacillus reuteri seed liquid ≥ 1×10 8 cfu / ml.

[0018] Preferably, in step (3), the fermentation temperature is 30 - 35 °C, and the fermentation time is 24 - 48 h.

[0019] Preferably, in step (3), the strain number of Bacillus subtilis is CGMCC 1.8886, and the strain number of Lactobacillus reuteri is CICC 6123.

[0020] In the second aspect of the present invention, there is provided the use of the above - mentioned composition for improving the production performance of hens in products for improving the production performance of hens.

[0021] Analysis of the effects of the plants selected in the present invention:

[0022] Leonurus japonicus is rich in alkaloids (such as leonurine), flavonoid compounds, polysaccharides and other substances, and has the effects of promoting blood circulation to remove blood stasis and regulating menstruation. By promoting pelvic and uterine blood circulation and regulating uterine smooth muscle contraction, it can improve the reproductive function of female poultry or livestock; the polysaccharides and some flavonoid components in Leonurus japonicus may have a certain promoting effect on the body's immune function and assist in enhancing disease resistance.

[0023] Spatholobus suberectus contains phytosterols, flavonoids, polyphenols, polysaccharides and other components, and has the following effects:

[0024] Tonifying blood and promoting blood circulation: By promoting the production of hemoglobin and red blood cells, it enhances the hematopoietic function of animals and improves the oxygen-carrying capacity of the blood;

[0025] Enhancing antioxidant capacity: Rich in polyphenol and flavonoid compounds, it can scavenge free radicals in the body and reduce oxidative damage to cells;

[0026] Immune enhancement: Some studies show that the extract of Spatholobus suberectus can improve the body's immune indexes (such as immunoglobulin levels) and improve the overall health status.

[0027] Quamoclit pennata contains flavonoids, alkaloids, saponins, polysaccharides and other components, and has the effect of antibacterial and anti-inflammatory. It has a certain inhibitory effect on a variety of common pathogenic bacteria, and can help reduce the inflammatory reaction in animals, especially playing an auxiliary preventive or relieving role in respiratory, digestive and reproductive tract diseases; in traditional Chinese veterinary medicine theory, Quamoclit pennata is slightly cold in nature, can clear heat and detoxify, and relieve swelling and pain, which helps to prevent and control heat-inflammatory diseases.

[0028] Advantages of the present invention:

[0029] 1. The present invention first combines Leonurus japonicus, Spatholobus suberectus and Quamoclit pennata. Leonurus japonicus focuses on reproductive health regulation, Spatholobus suberectus focuses on blood tonifying and antioxidant, and Quamoclit pennata focuses on antibacterial and anti-inflammatory. The advantages of the three are superimposed, which can specifically improve the reproductive function and overall health of hens, and solve the deficiency of single traditional Chinese medicine in broad-spectrum functions.

[0030] 2. The present invention uses multiple technical means such as ultrasonic extraction + probiotic fermentation to compound and match the advantages of the three herbs, realizing multi-functional synergistic effect, and having the characteristics of high efficiency, safety, and conforming to the trend of antibiotic-free breeding. Detailed implementation mode

[0031] It should be noted that the following detailed description is illustrative and is intended to provide further description of the present application. Unless otherwise specified, all technical and scientific terms used herein have the same meaning as commonly understood by those of ordinary skill in the technical field to which the present application belongs.

[0032] As described in the background art, the livestock and poultry breeding industry is facing the severe challenges of "banning antibiotics at the feed end and reducing antibiotics at the breeding end". Long-term reliance on antibiotics will not only lead to the generation and spread of drug-resistant strains, but also cause a series of problems such as drug residues and environmental pollution. Based on this, this application is committed to developing a safe and green composition that makes full use of plant-derived active substances and probiotic resources, can effectively improve the production performance of hens, and can reduce the risk of antibiotic use, so as to meet the urgent needs of the transformation of modern animal husbandry towards antibiotic-free and ecological directions.

[0033] In order to enable those skilled in the art to more clearly understand the technical solutions of this application, the technical solutions of this application will be described in detail below in conjunction with specific embodiments.

[0034] The test materials used in the embodiments of the present invention are all conventional test materials in the art and can be obtained through commercial channels.

[0035] The strain number of Bacillus subtilis used in the present invention is CGMCC 1.8886, which is purchased from the General Microbiology Center of the China Committee for Culture Collection of Microorganisms; the strain number of Lactobacillus reuteri is CICC 6123, which is purchased from the China Center of Industrial Culture Collection of Microorganisms.

[0036] Example 1: Preparation of a composition for improving the production performance of hens

[0037] 1. Raw material composition (parts by mass): 5 parts of motherwort-spatholobus suberectus composite extract and 1 part of ipomoea quamoclit extract;

[0038] 2. Preparation method:

[0039] Preparation of motherwort-spatholobus suberectus composite extract:

[0040] Motherwort and spatholobus suberectus were crushed according to a mass ratio of 4:1 to obtain a mixture, and the mixture was mixed evenly with an ethanol solution with a volume fraction of 80% according to a mass ratio of 1:10, and ultrasonically extracted 3 times. The time for each ultrasonic extraction was 15 min, the ultrasonic power was 200 W. After extraction, filtration was carried out, the extraction solutions were combined, concentrated, and dried to obtain the motherwort-spatholobus suberectus composite extract;

[0041] Preparation of ipomoea quamoclit extract:

[0042] (1) The whole herb of ipomoea quamoclit was dried and crushed to obtain ipomoea quamoclit powder;

[0043] (2) Distilled water with a mass 10 times that of the ipomoea quamoclit powder was added to the ipomoea quamoclit powder, and boiled for extraction for 1 h. After filtration, the filtrate was cooled to obtain an aqueous extract;

[0044] (3) Add Bacillus subtilis seed liquid and Lactobacillus reuteri seed liquid to the aqueous extract for fermentation. The fermentation temperature is 35 °C and the fermentation time is 48 h to obtain a fermentation broth.

[0045] The inoculation amount of Bacillus subtilis seed liquid is 3% of the volume of the aqueous extract, and the viable count of Bacillus subtilis seed liquid is 1×10 8 cfu / ml; The inoculation amount of Lactobacillus reuteri seed liquid is 3% of the volume of the aqueous extract, and the viable count of Lactobacillus reuteri seed liquid is 1×10 8 cfu / ml;

[0046] (4) Sterilize, concentrate and dry the fermentation broth to obtain Quamoclit pennata extract.

[0047] Example 2: Preparation of a composition for improving the production performance of hens

[0048] 1. Raw material composition (parts by mass): 7 parts of Leonurus japonicus - Spatholobus suberectus composite extract and 2 parts of Quamoclit pennata extract;

[0049] 2. Preparation method:

[0050] Preparation of Leonurus japonicus - Spatholobus suberectus composite extract:

[0051] Crush Leonurus japonicus and Spatholobus suberectus according to a mass ratio of 5:2 to obtain a mixture. Mix the mixture and an ethanol solution with a volume fraction of 85% evenly at a mass ratio of 1:12.5, perform ultrasonic extraction 3 times, with the ultrasonic extraction time of 15 min each time and the ultrasonic power of 200 W. After extraction, filter, combine the extraction solutions, concentrate and dry to obtain Leonurus japonicus - Spatholobus suberectus composite extract;

[0052] Preparation of Quamoclit pennata extract:

[0053] (1) Dry and crush the whole herb of Quamoclit pennata to obtain Quamoclit pennata powder;

[0054] (2) Add distilled water with a mass 15 times that of Quamoclit pennata powder to the Quamoclit pennata powder, boil and extract for 1.5 h. After filtration, cool the filtrate to obtain an aqueous extract;

[0055] (3) Add Bacillus subtilis seed liquid and Lactobacillus reuteri seed liquid to the aqueous extract for fermentation. The fermentation temperature is 35 °C and the fermentation time is 48 h to obtain a fermentation broth;

[0056] The inoculation amount of Bacillus subtilis seed liquid is 4% of the volume of the aqueous extract, and the viable count of Bacillus subtilis seed liquid is 1×10 8 cfu / ml; The inoculation amount of Lactobacillus reuteri seed liquid is 4% of the volume of the aqueous extract, and the viable count of Lactobacillus reuteri seed liquid is 1×10 8 cfu / ml;

[0057] (4) Sterilize, concentrate, and dry the fermentation broth to obtain the morning glory extract.

[0058] Example 3: Preparation of a composition for improving the production performance of hens

[0059] 1. Raw material composition (parts by mass): 9 parts of motherwort-spatholobus suberectus composite extract and 3 parts of morning glory extract;

[0060] 2. Preparation method:

[0061] Preparation of motherwort-spatholobus suberectus composite extract:

[0062] Crush motherwort and spatholobus suberectus according to a mass ratio of 6:3 to obtain a mixture. Mix the mixture and an ethanol solution with a volume fraction of 90% evenly according to a mass ratio of 1:15, perform ultrasonic extraction 3 times, with each ultrasonic extraction time being 15 min and the ultrasonic power being 200 W. After extraction, filter, combine the extracts, concentrate, and dry to obtain the motherwort-spatholobus suberectus composite extract;

[0063] Preparation of morning glory extract:

[0064] (1) Dry and crush the whole plant of Quamoclit pennata to obtain Quamoclit pennata powder;

[0065] (2) Add distilled water with a mass 20 times that of the Quamoclit pennata powder to the Quamoclit pennata powder, boil and extract for 2 h. After filtration, cool the filtrate to obtain the water extract;

[0066] (3) Add the Bacillus subtilis seed solution and the Lactobacillus reuteri seed solution to the water extract for fermentation. The fermentation temperature is 35 °C and the fermentation time is 48 h to obtain the fermentation broth;

[0067] The inoculation amount of the Bacillus subtilis seed solution is 5% of the volume of the water extract, and the viable count of the Bacillus subtilis seed solution is 1×10 8 cfu / ml; the inoculation amount of the Lactobacillus reuteri seed solution is 5% of the volume of the water extract, and the viable count of the Lactobacillus reuteri seed solution is 1×10 8 cfu / ml;

[0068] (4) Sterilize, concentrate, and dry the fermentation broth to obtain the morning glory extract.

[0069] Comparative Example 1: Preparation of motherwort extract

[0070] Crush motherwort and mix it evenly with an ethanol solution with a volume fraction of 80% according to a mass ratio of 1:10. Perform ultrasonic extraction 3 times, with each ultrasonic extraction time being 15 min and the ultrasonic power being 200 W. After extraction, filter, combine the extracts, concentrate, and dry to obtain the motherwort extract.

[0071] Comparative Example 2: Preparation of Millettia reticulata Benth. Extract

[0072] The Millettia reticulata Benth. was crushed and mixed evenly with an ethanol solution with a volume fraction of 80% at a mass ratio of 1:10, and ultrasonically extracted 3 times. The time for each ultrasonic extraction was 15 min, the ultrasonic power was 200 W. After extraction, it was filtered, the extracts were combined, concentrated, and dried to obtain the Millettia reticulata Benth. extract.

[0073] Comparative Example 3: Preparation of Quamoclit pennata (Desr.) Bojer Extract

[0074] (1) The whole herb of Quamoclit pennata (Desr.) Bojer was dried and crushed to obtain Quamoclit pennata (Desr.) Bojer powder;

[0075] (2) Distilled water with a mass 10 times that of the Quamoclit pennata (Desr.) Bojer powder was added thereto, and boiled for extraction for 1 h. After filtration, the filtrate was cooled to obtain the water extract;

[0076] (3) The water extract was added with Bacillus subtilis seed liquid and Lactobacillus reuteri seed liquid for fermentation. The fermentation temperature was 35 °C, and the fermentation time was 48 h to obtain the fermentation broth;

[0077] The inoculation amount of the Bacillus subtilis seed liquid was 3% of the volume of the water extract, and the viable count of the Bacillus subtilis seed liquid was 1×10 8 cfu / ml; the inoculation amount of the Lactobacillus reuteri seed liquid was 3% of the volume of the water extract, and the viable count of the Lactobacillus reuteri seed liquid was 1×10 8 cfu / ml;

[0078] (4) The fermentation broth was sterilized, concentrated, and dried to obtain the Quamoclit pennata (Desr.) Bojer extract.

[0079] Test Example 1:

[0080] 1.1 Test animals and grouping:

[0081] 150 1-day-old white - feather broilers with a body weight of 50 ± 5 g were selected and randomly divided into 4 treatment groups and a control group, with 3 replicates in each group.

[0082] Each treatment group was respectively:

[0083] Treatment group 1: 0.05% (mass fraction) of the composition for improving the production performance of hens prepared in Example 1 was added to the basal diet as the diet for feeding;

[0084] Treatment group 2: 0.05% (mass fraction) of the Leonurus japonicus Houtt. extract prepared in Comparative Example 1 was added to the basal diet as the diet for feeding;

[0085] Treatment group 3: 0.05% (mass fraction) of the Millettia reticulata Benth. extract prepared in Comparative Example 2 was added to the basal diet as the diet for feeding;

[0086] Four groups were treated: The extract of *Quamoclit pennata* prepared in Comparative Example 3 was added to the basal diet at 0.05% (mass fraction) as the diet for feeding;

[0087] Control group: Fed the basal diet;

[0088] Composition of the basal diet:

[0089] Corn 54.99%, soybean meal 34.15%, limestone powder 7.23%, soybean oil 0.50%, calcium hydrogen carbonate 2.09%, table salt 0.35%, trace element premix 0.30%, choline chloride (50%) 0.12%, methionine 0.17%, vitamin premix 0.10%.

[0090] The experimental period was 42 days. During the experimental period, all broiler chickens had free access to food and water, and were immunized according to the conventional immunization program. Except for the different diets fed, other feeding conditions were exactly the same.

[0091] 1.2 Determination indexes:

[0092] 1.2.1 Laying performance:

[0093] Eggs were collected regularly at 11:00, 14:30 and 17:00 every day. The number of eggs laid was recorded in units of replicates, and the egg weight (g) was weighed. The laying rate was calculated in units of weeks. The daily feed intake was counted every day, the spilled amount and the remaining amount were collected, and the daily total feed intake and feed-to-egg ratio were calculated.

[0094] Laying rate (%) = [Total number of eggs laid during the statistical period / (Number of hens raised × Number of days of raising)] × 100;

[0095] Daily total feed intake (g / d) = Feed intake - Spilled amount - Remaining amount;

[0096] Feed-to-egg ratio = Daily total feed intake / Daily total egg weight.

[0097] 1.2.2 Anti-inflammatory indexes:

[0098] On the 42nd day of the experiment, 6 white - feather broiler chickens with similar body weights were randomly selected from each treatment group (2 chickens per replicate) for wing - vein blood collection (after fasting for 12 h). After standing for 0.5 h, they were centrifuged for 15 min (3500 r / min). The upper - layer serum was aspirated with a pipette gun and stored at - 20 °C for measuring the anti - inflammatory indexes of the serum to be tested.

[0099] 1.3 Experimental results:

[0100] Table 1 Effects of different treatments on the laying performance of white - feather broiler chickens

[0101] Treatment Egg production rate (%) Egg-to-feed ratio Treatment 1 58.57 7.58 Treatment 2 44.44 5.31 Treatment 3 45.24 5.65 Treatment 4 43.65 5.46 Control group 40.48 4.79

[0102] As can be seen from Table 1, compared with the control group, feeding the composition for improving the production performance of hens prepared in Example 1 can improve the egg production rate of white - feather broilers and increase their feed - to - egg ratio. Feeding the extracts prepared in Comparative Examples 1 - 3 can also improve their egg production rate to varying degrees, but there is a large gap compared with Treatment 1.

[0103] Table 2 Effects of different treatments on anti - inflammatory indexes of white - feather broilers

[0104] Treatment TNF-α (pg / mL) IL-1β (pg / mL) IL-6 (pg / mL) Treatment 1 32.48 56.38 32.45 Treatment 2 54.29 81.65 49.36 Treatment 3 52.69 80.69 47.86 Treatment 4 46.26 72.36 42.63 Control group 58.46 88.95 51.34

[0105] As can be seen from Table 2, compared with the control group, feeding the composition for improving the production performance of hens prepared in Example 1 can reduce the contents of inflammatory factors TNF - α, IL - 1β, and IL - 6 in the serum of white - feather broilers, thereby slowing down the body's inflammatory response.

[0106] The above are only the preferred embodiments of the present application and are not used to limit the present application. For those skilled in the art, various changes and modifications can be made to the present application. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present application shall be included within the protection scope of the present application.

Claims

1. A composition for improving the production performance of hens, characterized in that, It comprises the following components in parts by mass: 5 - 9 parts of motherwort - millettia compound extract and 1 - 3 parts of morning glory extract.

2. The composition for improving the production performance of hens according to claim 1, wherein, The motherwort - millettia compound extract is prepared by the following method: Motherwort and millettia are crushed according to the mass ratio of (4 - 6):(1 - 3) to obtain a mixture, the mixture is mixed evenly with an ethanol solution with a volume fraction of 80% - 90% according to the mass ratio of 1:(10 - 15), ultrasonically extracted 2 - 4 times, filtered, the extracts are combined, concentrated, and dried to obtain the motherwort - millettia compound extract.

3. The composition for improving the production performance of hens according to claim 2, wherein The time for ultrasonic extraction is 15 - 20 min, and the ultrasonic power is 150 - 250 W.

4. The composition for improving the production performance of hens according to claim 1, wherein, The morning glory extract is prepared by the following method: (1) The whole herb of Quamoclit pennata is dried and crushed to obtain Quamoclit pennata powder; (2) Distilled water is added to the Quamoclit pennata powder, and it is extracted at 90 - 100 °C for 1 - 2 h. After filtration, the filtrate is cooled to obtain an aqueous extract; (3) Bacillus subtilis seed liquid and Lactobacillus reuteri seed liquid are added to the aqueous extract for fermentation to obtain a fermentation broth; (4) The fermentation broth is sterilized, concentrated, and dried to obtain the morning glory extract.

5. The composition for improving the production performance of hens according to claim 4, wherein In step (2), the mass ratio of Quamoclit pennata powder to distilled water is 1:(10 - 20).

6. The composition for improving the production performance of hens according to claim 4, wherein In step (3), the inoculation amount of Bacillus subtilis seed liquid is 3 - 5% of the volume of the aqueous extract, and the inoculation amount of Lactobacillus reuteri seed liquid is 3 - 5% of the volume of the aqueous extract.

7. The composition for improving the production performance of hens according to claim 6, characterized in that, In step (3), the viable count of the Bacillus subtilis seed solution ≥ 1×10 8 cfu / ml; the viable count of the Lactobacillus reuteri seed solution ≥ 1×10 8 cfu / ml.

8. The composition for improving the production performance of hens according to claim 4, characterized in that, In step (3), the fermentation temperature is 30 - 35 °C, and the fermentation time is 24 - 48 h.

9. The composition for improving the production performance of hens according to claim 4, characterized in that, In step (3), the strain number of Bacillus subtilis is CGMCC 1.8886, and the strain number of Lactobacillus reuteri is CICC 6123.

10. Use of the composition for improving the production performance of hens according to any one of claims 1 - 9 in a product for improving the production performance of hens.