Traditional Chinese medicine composition for preventing and treating ketosis of dairy cows, oral liquid, preparation method and application thereof

Abstract

The application provides a traditional Chinese medicine composition for preventing and treating ketosis of dairy cows, an oral liquid, a preparation method and application thereof, and belongs to the field of veterinary products. Active ingredients of the application include the following raw materials in parts by weight: 30-40 parts of rhizoma polygonati, 20-35 parts of cortex lycii, 15-25 parts of curcuma, 15-25 parts of rehmannia glutinosa, 5-15 parts of dioscorea, 5-15 parts of chicory, 5-15 parts of taraxacum, 5-10 parts of motherwort, and 5-10 parts of citrus reticulata. The composition of the application is reasonably matched, has synergistic effects, fully plays the role of each medicine, and has obvious effects on preventing and treating ketosis of dairy cows.

Description

Technical Field

[0001] This invention relates to the field of veterinary drug technology, specifically to a traditional Chinese medicine composition for the prevention and treatment of ketosis in dairy cows, an oral liquid of the traditional Chinese medicine composition, and its preparation method and application. Background Technology

[0002] Ketosis is a common nutritional and metabolic disease in dairy cows during the peripartum period, with an incidence rate ranging from 28.5% to 60%. During this period, dairy cows undergo significant physiological and metabolic changes, such as insulin resistance, reduced feed intake, decreased immune function, and negative energy balance. When energy intake cannot meet the daily energy needs of dairy cows in early lactation, a negative energy balance occurs. Cows in a state of negative energy balance promote fat breakdown, resulting in a large amount of non-esterified fatty acids (NEFAs) entering the bloodstream. When the level of NEFAs in the blood exceeds the liver's metabolic capacity, incomplete oxidation metabolites such as ketone bodies are generated. High ketone levels put dairy cows in a sub-healthy state, making them highly susceptible to ketosis, fatty liver disease, and secondary complications such as postpartum paralysis, retained placenta, and metritis, seriously threatening the health of dairy cows. Furthermore, affected cows not only experience decreased production performance and reduced milk quality, but their reproductive performance is also affected, shortening their productive lifespan and causing significant economic losses to farms.

[0003] With the rapid development of the dairy industry, the incidence of ketosis in dairy cows is also increasing. After developing ketosis, the cow will experience decreased appetite, reduced lactation, impaired liver function, and weakened immune system, and is more likely to develop various diseases. A typical lactation period for a dairy cow is approximately 305 days. Ketosis can lead to a loss of up to 200 kg of milk during the entire lactation period. At a market milk price of 3-4 yuan, the economic loss due to reduced milk production alone during the entire lactation period can reach approximately 800 yuan. Simultaneously, ketosis causes a decline in milk quality, specifically a decrease in milk fat and protein content; it delays the onset of estrus, leads to an increase in mating frequency, weakened ovarian activity, and a lower conception rate, thus significantly reducing the reproductive performance of dairy cows, prolonging the reproductive cycle of affected cows, and causing economic losses that can reach up to approximately 5000 yuan. Dairy cow ketosis seriously threatens the healthy development of the dairy industry. Effective prevention and treatment of dairy cow ketosis has become an urgent problem to be solved.

[0004] Currently, propylene glycol and glucose are commonly used in clinical practice for prevention and treatment. However, oral administration of propylene glycol has poor palatability and can cause stress in dairy cows. Developing new traditional Chinese medicine (TCM) preparations is the ideal choice. Currently, herbs such as licorice, tangerine peel, amomum villosum, red peony root, and medicated leaven are used in ruminants. From a TCM perspective, ketosis belongs to the category of deficiency syndrome, specifically qi deficiency, often caused by dietary imbalances and physical weakness. Clinical manifestations include fatigue, loss of appetite, and decline in organ function. Treatment should focus on tonifying qi and strengthening the spleen, promoting blood circulation, nourishing yin and tonifying the kidneys, replenishing the middle qi, and promoting healthy function. Based on the current characteristics of ketosis in dairy cows, a TCM composition with good clinical efficacy and cost-effectiveness for the prevention and treatment of ketosis in dairy cows should be selected. Summary of the Invention

[0005] Purpose of the invention

[0006] The purpose of this invention is to provide a traditional Chinese medicine composition for the prevention and treatment of ketosis in dairy cows, an oral liquid of the traditional Chinese medicine composition, its preparation method and application. The composition of this invention has mild medicinal properties, invigorates qi and strengthens the spleen, promotes blood circulation and nourishes blood, nourishes yin and tonifies the kidneys, and effectively replaces drugs such as propylene glycol and glucose.

[0007] Solution

[0008] To achieve the objectives of this invention, the technical solution adopted is as follows:

[0009] In a first aspect, the present invention provides a traditional Chinese medicine composition for the prevention and treatment of ketosis in dairy cows, the active ingredients of which include the following raw materials in parts by weight: 30-40 parts of Polygonatum sibiricum, 20-35 parts of Lycium chinense root bark, 15-25 parts of Curcuma longa, 15-25 parts of Rehmannia glutinosa (processed), 5-15 parts of Dioscorea opposita, 5-15 parts of Chicory, 5-15 parts of Taraxacum mongolicum, 5-10 parts of Leonurus japonicus, and 5-10 parts of Citronella arvense bark.

[0010] Furthermore, its active ingredients include the following composition in parts by weight: 30-35 parts of Polygonatum sibiricum, 20-30 parts of Lycium chinense root bark, 20-25 parts of Curcuma longa, 20-25 parts of Rehmannia glutinosa (processed), 10-15 parts of Dioscorea opposita, 10-15 parts of Chicory, 10-15 parts of Taraxacum mongolicum, 5-7.5 parts of Leonurus japonicus, and 5-7.5 parts of Citronella arvense bark.

[0011] Furthermore, its active ingredients include the following composition in parts by weight: 35 parts Polygonatum sibiricum, 30 parts Lycium chinense root bark, 20 parts Curcuma longa, 20 parts Rehmannia glutinosa (processed), 10 parts Dioscorea opposita, 10 parts Chicory, 10 parts Taraxacum mongolicum, 7.5 parts Leonurus japonicus, and 7.5 parts Citronella arvense bark.

[0012] Furthermore, the dosage form of the traditional Chinese medicine composition is any one of oral liquid, powder, spray, ointment, injection, and capsule; optionally, it is an oral liquid.

[0013] Furthermore, the amount of raw medicinal material in the oral liquid is 0.5–2 g / mL, optionally 1 g / mL.

[0014] In a second aspect, a traditional Chinese medicine composition oral liquid is provided, prepared according to the traditional Chinese medicine composition described in the first aspect.

[0015] Thirdly, a method for preparing the traditional Chinese medicine composition described in the first aspect or the oral liquid of the traditional Chinese medicine composition described in the second aspect is provided, comprising taking each medicinal material in parts by weight, pulverizing, decocting, and concentrating to obtain the product.

[0016] Further including:

[0017] 1) Take each medicinal material according to the stated weight proportions, crush and sieve; pass through a No. 2 sieve;

[0018] 2) Take 12 to 15 times the total weight of all the medicinal materials in 1) and add water to the sieved raw materials in 1), and optionally decoct for 2 to 4 hours;

[0019] 3) Filter the Chinese medicine after decocting in 2), add 8 to 10 times the total weight of the Chinese medicine to the filter residue and decoct for 1 to 2 hours.

[0020] 4) Filter the Chinese medicine after decocting in 3), then add 6 to 8 times the total weight of the Chinese medicine and decoct for 0.5 to 3 hours.

[0021] 5) Combine the filtered liquids from 2), 3), and 4), concentrate the liquid to a raw drug concentration of 0.5–2 g / mL, and sterilize to prepare the oral liquid.

[0022] Furthermore, in step 2), the raw medicinal materials are soaked for 2-3 hours before decoction.

[0023] And / or, in steps 2), 3), and 4), after boiling, simmer over low heat for 2 hours.

[0024] And / or, in step 5), after the filtered drug solutions are combined, they are first allowed to stand for 6 to 10 hours, and then the supernatant is collected by centrifugation and concentrated.

[0025] Fourthly, a cattle lick feed is provided, which also includes the traditional Chinese medicine composition described in the first aspect, or the oral liquid of the traditional Chinese medicine composition described in the second aspect, or the product prepared by the preparation method described in the third aspect.

[0026] Fifthly, the application of the traditional Chinese medicine composition described in the first aspect, or the powder of the traditional Chinese medicine composition described in the second aspect, or the product prepared by the preparation method described in the third aspect, or the bovine lick feed described in the fourth aspect, in the preparation of products for the prevention or treatment of ketosis in dairy cows, optionally, the product is a drug.

[0027] Beneficial effects

[0028] In this invention, Polygonatum sibiricum and Rehmannia glutinosa are the principal herbs, working together to invigorate qi and strengthen the spleen, nourish blood and yin. Curcuma longa and Dioscorea opposita are added to promote blood circulation and tonify the kidneys. Lycium chinense root bark, Taraxacum mongolicum, and Leonurus japonicus are used as adjuvant herbs, working together to clear heat and detoxify, invigorate blood and remove blood stasis. Chicory and Citronella arvense are used as guiding herbs, working together to strengthen the spleen and stomach, promote digestion and relieve stagnation. The formulation of this invention is rationally combined, synergistically enhancing the efficacy of each herb, and fully utilizing their effects, demonstrating significant efficacy in preventing and treating ketosis in dairy cows.

[0029] The herbal composition of this invention allows each ingredient to work synergistically for optimal efficacy, providing a reference for the treatment of ketosis in dairy cows using traditional Chinese veterinary medicine. The herbal composition of this invention is mild in nature, capable of tonifying qi and spleen, promoting blood circulation and replenishing blood, nourishing yin and tonifying the kidneys, promoting digestion, and enhancing appetite. The synergistic effects of each ingredient effectively replace drugs such as propylene glycol and glucose, reducing stress in dairy cows, making it an ideal traditional Chinese veterinary medicine preparation. Attached Figure Description

[0030] One or more embodiments are illustrated by way of example with reference to the accompanying drawings, and these illustrative examples are not intended to limit the embodiments. The term "illustrative" as used herein means "serving as an example, embodiment, or illustration." Any embodiment illustrated herein as "illustrative" is not necessarily to be construed as superior to or better than other embodiments.

[0031] Figure 1 This invention relates to a blood collection diagram from the calf tail vein.

[0032] Figure 2 The blood ketone meter of this invention measures blood ketone levels.

[0033] Figure 3 This is a graph showing the original data of blood ketone levels in this invention.

[0034] Figure 4 The images show the experimental cattle administered the medicine and the experimental samples of this invention.

[0035] Figure 5 This is a diagram of blood ketone level detection and infusion in this invention. Detailed Implementation

[0036] To make the objectives, technical solutions, and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below. Obviously, the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0037] Furthermore, to better illustrate the present invention, numerous specific details are provided in the following detailed embodiments. Those skilled in the art should understand that the present invention can be practiced without certain specific details. In some embodiments, materials, elements, methods, and means well known to those skilled in the art are not described in detail in order to highlight the spirit of the invention.

[0038] The traditional Chinese medicine for preventing and treating ketosis in dairy cows of this invention comprises: Polygonatum sibiricum, Lycium chinense root bark, Curcuma longa, Rehmannia glutinosa (processed), Dioscorea opposita, Chicorydalis yanhusuo, Taraxacum mongolicum, Leonurus japonicus, and Citronella arvense bark, wherein...

[0039] Polygonatum sibiricum; neutral in nature, sweet in taste, and enters the spleen, lung, and kidney meridians. It has the effects of tonifying qi and nourishing yin, strengthening the spleen, moistening the lungs, and benefiting the kidneys. Modern pharmacological research suggests that Polygonatum sibiricum possesses antioxidant activity, regulates immunity, nourishes nerves, enhances memory, lowers blood sugar and lipids, protects bones, has anti-inflammatory and antibacterial properties, and has anti-fatigue and anti-cancer effects.

[0040] Lycium chinense root bark; bitter in taste, cold in nature, and enters the heart and liver meridians. It has the effects of clearing heat, cooling blood, and detoxifying. Modern pharmacology believes that Lycium chinense root bark has various pharmacological effects, including antimicrobial activity, antipyretic effect, hypoglycemic effect, and hypolipidemic effect.

[0041] Turmeric; cold in nature, pungent and bitter in taste, and enters the liver, heart, and lung meridians. It has the effects of promoting blood circulation and relieving pain, regulating qi and relieving depression, clearing the heart and cooling the blood, and promoting bile secretion and reducing jaundice. Modern pharmacology believes that turmeric can relieve qi stagnation and blood stasis, improve liver qi stagnation and heat, protect the liver and promote bile secretion, prevent infectious hepatitis, and resist arteriosclerosis.

[0042] Rehmannia glutinosa (processed); warm in nature, sweet and bitter in taste. It has the effects of nourishing blood and yin, replenishing marrow and nourishing the spirit, enhancing memory and immunity, and lowering blood sugar. Modern pharmacology believes that Rehmannia glutinosa has the effects of nourishing yin and kidney, replenishing blood and calming the mind, clearing heat and detoxifying, anti-aging, and anti-tumor effects.

[0043] Yam; neutral in nature and sweet in taste. It enters the Hand and Foot Taiyin meridians. It has the effects of strengthening the spleen, tonifying the lungs, consolidating the kidneys, benefiting essence, enhancing the absorption function of the small intestine, aiding digestion, and protecting against gastric mucosal damage. Modern pharmacological research suggests that yam has the effects of tonifying the spleen and stomach, promoting body fluid production and benefiting the lungs, tonifying the kidneys and astringing essence, lowering blood lipids, and anti-tumor activity.

[0044] Chicory is cool in nature, slightly bitter and salty in taste, and enters the liver, gallbladder, and stomach meridians. Its functions and indications include clearing the liver and gallbladder, strengthening the stomach and aiding digestion, and promoting diuresis and reducing swelling. Modern pharmacology suggests it can promote digestion and lower blood sugar.

[0045] Dandelion; cold in nature, sweet in taste, entering the Yangming and Taiyin meridians. It has the effects of clearing heat and detoxifying, promoting diuresis and resolving lumps. Modern pharmacology believes that it has a good killing effect on Helicobacter pylori, can clear the blockage of mammary ducts, promote lactation, and has anti-tumor and choleretic effects.

[0046] Motherwort; slightly cold in nature, bitter and pungent in taste. It enters the liver, heart, and bladder meridians. Motherwort has the functions of promoting blood circulation and removing blood stasis, regulating menstruation and eliminating edema. It is used to treat irregular menstruation, threatened abortion, difficult labor, retained placenta, postpartum blood stasis and abdominal pain, metrorrhagia, hematuria, hematochezia, painful swellings and sores, etc.

[0047] Citron peel is cold in nature, bitter, sour, and enters the liver, lung, and spleen meridians. It has anti-inflammatory, antiviral, spleen-strengthening, stomach-nourishing, analgesic, liver-soothing, and qi-regulating effects. Modern pharmacology suggests that citron peel can soothe the liver, relieve depression, resolve phlegm, and stop coughing in cases of indigestion, abdominal distension, loss of appetite, nausea, and vomiting caused by spleen and stomach weakness.

[0048] Feasible embodiments of the traditional Chinese medicine composition of the present invention are as follows:

[0049] Example 1

[0050] Polygonatum 3.5kg, Lycium bark 3kg, Curcuma longa 2kg, Rehmannia glutinosa 2kg, Dioscorea opposita 1kg, Chicory 1kg, Taraxacum mongolicum 1kg, Leonurus japonicus 0.75kg, Citronella bark 0.75kg.

[0051] Example 2

[0052] 4 kg of Polygonatum sibiricum, 2 kg of Lycium chinense root bark, 1.5 kg of Curcuma longa, 1.5 kg of Rehmannia glutinosa (processed), 0.75 kg of Dioscorea opposita, 0.75 kg of Chicory, 0.75 kg of Taraxacum mongolicum, 0.75 kg of Leonurus japonicus, and 0.75 kg of Citronella tamariscina bark.

[0053] Example 3

[0054] 3 kg of Polygonatum sibiricum, 3 kg of Lycium chinense root bark, 2.5 kg of Curcuma longa, 2.5 kg of Rehmannia glutinosa (processed), 1.5 kg of Dioscorea opposita, 1.5 kg of Chicory, 1.5 kg of Taraxacum mongolicum, 0.5 kg of Leonurus japonicus, and 0.5 kg of Citronella tamariscina bark.

[0055] Example 4

[0056] 2kg of Polygonatum sibiricum, 2kg of Lycium chinense root bark, 1.5kg of Curcuma longa, 1.5kg of Rehmannia glutinosa (processed), 0.75kg of Dioscorea opposita, 0.75kg of Chicory, 0.75kg of Taraxacum mongolicum, 0.5kg of Leonurus japonicus, and 0.5kg of Citronella tamariscina bark.

[0057] Example 5

[0058] 3 kg of Polygonatum sibiricum, 3 kg of Lycium chinense root bark, 2 kg of Curcuma longa, 2 kg of Rehmannia glutinosa (processed), 1.5 kg of Dioscorea opposita, 1.5 kg of Chicory, 1.5 kg of Taraxacum mongolicum, 0.75 kg of Leonurus japonicus, and 0.75 kg of Citronella tamariscina bark.

[0059] Comparative Example 1

[0060] The difference between this embodiment and Embodiment 1 lies in the raw materials and proportions: 5 kg of Polygonatum sibiricum, 2 kg of Lycium chinense root bark, 2.5 kg of Curcuma longa, 2.5 kg of Rehmannia glutinosa (processed), 1.5 kg of Dioscorea opposita, 1.5 kg of Chicory, 1.5 kg of Taraxacum mongolicum, 0.5 kg of Angelica sinensis, and 0.5 kg of Citronella tamariscina.

[0061] Comparative Example 2

[0062] The difference between this embodiment and Embodiment 1 lies in the raw materials and proportions: 5 kg of Polygonatum sibiricum, 2 kg of Lycium chinense root bark, 1.5 kg of Curcuma longa, 1.5 kg of Rehmannia glutinosa (processed), 0.75 kg of Astragalus membranaceus, 1 kg of Chicory, 1 kg of Taraxacum mongolicum, 1 kg of Leonurus japonicus, and 1 kg of Citronella tamariscina.

[0063] Comparative Example 3

[0064] The difference between this embodiment and Embodiment 1 lies in the raw materials and proportions: 2 kg of Polygonatum sibiricum, 1.5 kg of Lycium chinense root bark, 2.5 kg of Curcuma longa, 2.5 kg of Rehmannia glutinosa (processed), 1.5 kg of Dioscorea opposita, 1.5 kg of Chicory, 1.5 kg of Taraxacum mongolicum, 1 kg of Leonurus japonicus, and 1 kg of Poria cocos.

[0065] Comparative Example 4

[0066] The difference between this embodiment and Embodiment 1 lies in the raw materials and proportions: 1 kg of Polygonatum sibiricum, 2 kg of Lycium chinense root bark, 1.5 kg of Curcuma longa, 1 kg of Dioscorea opposita, 1 kg of Chicory, 1 kg of Taraxacum mongolicum, 0.5 kg of Leonurus japonicus, and 0.5 kg of Citronella bark.

[0067] Comparative Example 5

[0068] The publicly disclosed traditional Chinese medicine composition includes: Astragalus membranaceus 180 parts, Codonopsis pilosula 50 parts, stir-fried Atractylodes macrocephala 50 parts, Poria cocos 50 parts, prepared Glycyrrhiza uralensis 50 parts, Angelica sinensis 60 parts, Artemisia capillaris 100 parts, Gardenia jasminoides 30 parts, charred Crataegus pinnatifida 60 parts, charred Hordeum vulgare 60 parts, charred Massa fermentata 60 parts, Citrus aurantium 50 parts, Sparganium stoloniferum 30 parts, Curcuma zedoaria 15 parts, Curcuma longa 15 parts, Sanguisorba officinalis 20 parts, and Rheum palmatum 15 parts. Its preparation method includes:

[0069] S1: Grind the components of the feed additive and mix them thoroughly to obtain a traditional Chinese medicine composition;

[0070] S2: Use 2 x 10g of brewing yeast at a 1% mass ratio. 8 CFU / g, Aspergillus oryzae 1*10 5 CFU / g, Aspergillus niger 1*10 6 CFU / g, Trichoderma viride 1*10 6 CFU / g and 2*10 lactic acid bacteria 7 The traditional Chinese medicine composition was anaerobic fermented for 7 days using a compound microorganism of CFU / g and 5% corn flour by mass as a base (each microorganism is a commercially available microorganism).

[0071] Preparation of drug solution

[0072] 1) Mix the raw materials of Examples 1-5 and Comparative Examples 1-5 according to the specified proportions. Crush and sieve; pass through a No. 2 sieve.

[0073] 2) Take 12 to 15 times the total weight of all the medicinal materials in 1) and soak the raw materials that have been sieved in the first step for 2 to 3 hours; then boil them and simmer for 2 hours.

[0074] 3) Filter the Chinese medicine from step 2), and add 8 to 10 times the total weight of the medicinal materials to the filter residue and decoct for 1 to 2 hours;

[0075] 4) Filter the Chinese herbs from step 3), then add 6 to 8 times the total weight of the herbs to water and decoct for 0.5 to 3 hours;

[0076] 5) Combine the decoctions from the three decoctions in steps 2), 3), and 4), let stand overnight, centrifuge and collect the supernatant, concentrate the decoction to a raw drug concentration of 1 g / mL, sterilize and prepare the oral liquids of Examples 1-5 and Comparative Examples 1-5 respectively, and store them in a refrigerator at 2-8℃ for later use.

[0077] Clinical Trial Example 1: Clinical Efficacy Trial of Cadence in Dairy Bovine Ketosis

[0078] 1.1 Materials

[0079] Experimental dairy cows: Holstein dairy cows from a dairy farm in Baoding, Hebei Province.

[0080] 1.2 Diet and Feeding

[0081] The cattle were fed the normal diet according to the farmers' standards, without any treatment; throughout the experimental period, the experimental cattle were fed normally, and the feeding frequency and drinking water were not adjusted.

[0082] 1.3 Measurement and Methods of Ketosis Indicators in Dairy Bovines

[0083] A ketone level of 1.2 mmol / L or higher is considered ketosis. A ketone level below 1.2 mmol / L after three oral drenchings indicates a cure. Blood was drawn from the tail vein of the affected cattle before treatment, and the blood ketone level was measured using a blood ketone meter. After three oral drenchings, blood was drawn again to measure the ketone level. Photos of blood collection and oral drenching are shown below. Figures 1-5 As shown.

[0084] 1.4 Animal Experiments

[0085] Experimental grouping: 120 cattle with hemoketone disease were selected from a dairy farm in Baoding, Hebei Province, and randomly divided into 12 groups: 1 drug control group, 1 model control group, and 10 traditional Chinese medicine compound groups (Example 1-5 groups and Comparative Example 1-5 groups), with 10 cattle in each group. The blood ketone values ​​of each diseased cattle were measured using a blood ketone meter. The results are shown in Table 1.

[0086] Table 1. Mean serum ketone levels (mmol / L) of each group before treatment.

[0087] Group Mean blood ketone levels before treatment (mmol / L) Group Mean blood ketone levels before treatment (mmol / L) Drug control group 2.38 Model control group 2.43 Example 1 2.42 Comparative Example 1 2.41 Example 2 2.32 Comparative Example 2 2.46 Example 3 2.44 Comparative Example 3 2.35 Example 4 2.47 Comparative Example 4 2.49 Example 5 2.37 Comparative Example 5 2.39

[0088] The following treatment plans were adopted respectively:

[0089] Drug control group (routine technical program on the ranch): Starting from the day ketosis was detected, each animal was given intravenous fluids (1000ml of 50% glucose, 1000ml of 10% calcium gluconate, 500ml of 10% saline, and 100ml of hydrocortisone) and 300ml of propylene glycol was administered orally for 3 consecutive days.

[0090] Model control group: Starting from the day ketosis was detected, each cow was given 500 mL of physiological saline by gavage for 3 consecutive days.

[0091] The cows in the traditional Chinese medicine compound groups 1-5 were given the oral liquid medicine prepared in Examples 1-5, respectively; the cows in the traditional Chinese medicine compound groups 6-9 were given the oral liquid medicine prepared in Comparative Examples 1-4 by gavage, 500ml each, for 3 consecutive days; the cows in the traditional Chinese medicine compound groups 10 were given the powder medicine prepared in Comparative Example 6 by gavage, 500g each, for 3 consecutive days.

[0092] During the observation period, the activity and mental state of the dairy cows in each group were observed, and the blood ketone levels of the dairy cows were recorded 3 days after administration. The results are shown in Tables 2-4.

[0093] Table 2. Mean serum ketone levels (mmol / L) in each group after treatment.

[0094] Group Mean blood ketone levels after treatment (mmol / L) Group Mean blood ketone levels after treatment (mmol / L) Drug control group 0.98 Model control group 2.59 Example 1 1.18 Comparative Example 1 1.87 Example 2 1.8 Comparative Example 2 1.84 Example 3 1.85 Comparative Example 3 1.78 Example 4 1.75 Comparative Example 4 1.98 Example 5 1.74 Comparative Example 5 1.45

[0095] Table 3. Serum ketone levels (mmol / L) before and after treatment for ketosis.

[0096] Grouping Pre-treatment blood ketone levels Post-treatment blood ketone levels Blood ketone difference Drug control group 2.38±0.32 <![CDATA[0.98±0.68 A ]]> <![CDATA[1.40±0.63 A ]]> Example 1 2.42±0.30 <![CDATA[1.18±0.66 A ]]> <![CDATA[1.24±0.82 A ]]> Example 2 2.32±0.38 1.80±1.01 0.52±1.15 Example 3 2.44±0.31 1.85±0.97 0.59±1.04 Example 4 2.47±0.38 1.75±0.93 0.72±0.92 Example 5 2.37±0.30 1.74±1.12 0.63±0.99 Model control group 2.43±0.31 <![CDATA[2.59±1.06 Bb ]]> <![CDATA[-0.16±1.21 Bb ]]> Comparison Column 1 2.41±0.33 1.87±1.27 0.54±1.16 Comparison Column 2 2.46±0.37 1.84±1.06 0.62±0.97 Comparison Column 3 2.35±0.33 1.78±1.19 0.57±1.08 Comparison Column 4 2.49±0.39 1.98±1.04 0.51±1.04 Comparison Column 5 2.39±0.38 <![CDATA[1.45±0.74 a ]]> <![CDATA[0.94±0.76 a ]]>

[0097] Note: Different lowercase letters in the same column indicate significant differences (P<0.05), different uppercase letters in the same column indicate extremely significant differences (P<0.01), and the same letter or no letter in the same column indicates no significant differences (P>0.05).

[0098] Table 4. Cure rate of ketosis in dairy cows

[0099]

[0100] 1.5. Data Analysis: Data were statistically analyzed using SPSS 19.0 statistical software.

[0101] 1.6. Experimental Results: As shown in Table 3, the blood ketone levels of the dairy cows in Example 1 group after treatment were lower than those in other examples and the comparative group. The differences between Example 1 group, the drug control group, and the model control group were extremely significant. This indicates that the traditional Chinese medicine compound provided by this invention can effectively treat ketosis in dairy cows and can effectively replace drugs such as propylene glycol and glucose. As shown in Table 4, the cure rate of ketosis in dairy cows in Example 1 group was higher than that in other examples, the comparative group, and the model control group.

[0102] The above experiments show that the traditional Chinese medicine composition of the present invention can effectively cure ketosis in dairy cows.

[0103] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention, and not to limit them; although the present invention has been described in detail with reference to the foregoing embodiments, those skilled in the art should understand that modifications can still be made to the technical solutions described in the foregoing embodiments, or equivalent substitutions can be made to some of the technical features; and these modifications or substitutions do not cause the essence of the corresponding technical solutions to deviate from the spirit and scope of the technical solutions of the embodiments of the present invention.

Claims

1. A traditional Chinese medicine composition for preventing and treating ketosis in dairy cows, characterized in that, it comprises the following components: The active ingredients thereof are composed of raw materials in the following weight parts: 30-40 parts of Rhizoma Polygonati, 20-35 parts of Pericarpium Lycii, 15-25 parts of Radix Anemarrhena, 15-25 parts of Radix Rehmanniae Preparata, 5-15 parts of Dioscoreae, 5-15 parts of Herba Taraxaci, 5-15 parts of Herba Leonuri, 5-10 parts of Herba Motherwort, and 5-10 parts of Citrus Peel. ​ 2. The traditional Chinese medicine composition according to claim 1, characterized in that, The active ingredients thereof are composed of raw materials in the following weight parts: 30-40 parts of Rhizoma Polygonati, 20-35 parts of Pericarpium Lycii, 15-25 parts of Radix Anemarrhena, 15-25 parts of Radix Rehmanniae Preparata, 5-15 parts of Dioscoreae, 5-15 parts of Herba Taraxaci, 5-15 parts of Herba Leonuri, 5-10 parts of Herba Motherwort, and 5-10 parts of Citrus Peel.

3. The traditional Chinese medicine composition according to claim 1, characterized in that, The active ingredients thereof are composed of raw materials in the following weight parts: 30-40 parts of Rhizoma Polygonati, 20-35 parts of Pericarpium Lycii, 15-25 parts of Radix Anemarrhena, 15-25 parts of Radix Rehmanniae Preparata, 5-15 parts of Dioscoreae, 5-15 parts of Herba Taraxaci, 5-15 parts of Herba Leonuri, 5-10 parts of Herba Motherwort, and 5-10 parts of Citrus Peel.

4. The traditional Chinese medicine composition according to any one of claims 1 to 3, characterized in that, The dosage form of the traditional Chinese medicine composition is any one of oral liquid, powder, spray, ointment, injection, and capsule.

5. The traditional Chinese medicine composition according to any one of claims 1 to 3, characterized in that, The dosage form of the traditional Chinese medicine composition is oral liquid.

6. The traditional Chinese medicine composition according to claim 5, characterized in that, The crude drug amount of the oral liquid is 0.5-2 g / mL.

7. The traditional Chinese medicine composition according to claim 5, characterized in that, The crude drug amount of the oral liquid is 1 g / mL.

8. A traditional Chinese medicine composition oral liquid, characterized in that, The traditional Chinese medicine composition prepared according to any one of claims 1-7.

9. A method for preparing the traditional Chinese medicine composition of any one of claims 1 to 7 or the oral liquid of the traditional Chinese medicine composition of claim 8, characterized in that, Comprising: The raw medicinal materials are taken in the weight parts, crushed, decocted, and concentrated.

10. The method of claim 9, wherein, Comprising: 1) The raw medicinal materials are taken in the weight parts, crushed, and sieved; 2) Water with a weight of 12-15 times the total weight of the raw medicinal materials in 1) is taken, and the crushed raw medicinal materials in 1) are added; 3) The traditional Chinese medicine decocted in 2) is filtered, and the filter residue is again decocted with water with a weight of 8-10 times the total weight of the raw medicinal materials; 4) The traditional Chinese medicine decocted in 3) is filtered, and the filter residue is again decocted with water with a weight of 6-8 times the total weight of the raw medicinal materials; 5) The filtered medicinal liquids in 2), 3), and 4) are combined, concentrated to a crude drug amount of 0.5-2 g / mL, and sterilized to prepare the oral liquid.

11. The method of claim 10, wherein, In step 2), the decoction is performed for 2-4 h.

12. The method of claim 10, wherein, In step 3), the decoction is performed for 1-2 h.

13. The preparation method according to claim 10, characterized in that, In step 4), the decoction is performed for 0.5-3 h.

14. The production method according to any one of claims 10 to 13, characterized by, In step 2), the raw medicinal materials are soaked for 2-3 h before decoction.

15. The production method according to any one of claims 10 to 13, characterized by, In steps 2), 3), and 4), after the decoction is boiled, the decoction is performed with a low heat for 2 h.

16. The method of making according to any one of claims 10 to 13, wherein, In step 5), after the filtered medicinal liquids are combined, they are first left to stand for 6-10 h, and then centrifuged to obtain the supernatant for concentration.

17. A bovine lick supplement characterized in that, The product prepared by the traditional Chinese medicine composition according to any one of claims 1-7, or the oral liquid of the traditional Chinese medicine composition according to claim 8, or the preparation method according to any one of claims 9-16.

18. Use of the product prepared by the traditional Chinese medicine composition according to any one of claims 1-7, or the oral liquid of the traditional Chinese medicine composition according to claim 8, or the preparation method according to any one of claims 9-16, or the cattle lick prepared by the method according to claim 9, in the preparation of a product for preventing or treating ketosis in dairy cows.

19. The use according to claim 18, characterized in that, The product is a medicine.

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