Feed composition for improving reproductive performance of sows by using 25-OH-VD3 and preparation method thereof

Abstract

The invention relates to the technical field of feed additives, and discloses a feed combination agent for improving the reproductive performance of sows by using 25-OH-VD3 and a preparation method thereof, and the preparation method comprises the following steps: taking an innovative product 25-OH-VD3 as a main raw material, and optimally combining other micronutrients, immune substances, metabolism promoting substances and the like for cooperative utilization to prepare the combination agent. The innovative 25-OH-VD3 not only serves as a golden partner for calcium and phosphorus metabolism of an organism, but also plays a wide immune regulation function, improves the reproductive function of pigs together and strengthens an optimized feed combination method and composition of the main effect of the 25-OH-VD3 at the same time, and other vitamins, trace elements, targeted active substances and other optimized combinations are preferably selected. The feed mainly solves the technical problem of integration of health and reproductive disorder of modern pig raising, and has the effects of remarkably enhancing the immunity of the sows, resisting oxidation and stress and systematically improving the reproductive performance of the sows.

Description

Technical Field

[0001] The invention relates to the technical field of feed additives, in particular to a feed composition for improving sow reproductive performance by utilizing 25-OH-VD3 and a preparation method thereof. Background Art

[0002] The factors that affect the reproductive performance of breeding pigs include breed, nutrition, management, and disease. However, in the modern large-scale intensive breeding model, excellent breeds and standardized management procedures have played an important role in promoting the reproductive performance of breeding pigs. However, high-density breeding has brought about the problems of decreased immunity and general sub-health in live pigs, which has become an important obstacle to improving their reproductive performance. Systematically solving the problems of pig immunosuppression, sub-health, and reproductive disorders through nutritional immunity is the most realistic and sustainable practical strategy for reducing costs and increasing efficiency in modern pig farming.

[0003] 25-OH-VD3 (25-OH-VD3) is vitamin D3 activated by the liver in animals. Traditional vitamin D utilization mainly focuses on its "golden partner" effect in calcium and phosphorus metabolism in livestock and poultry. However, many studies in recent years have shown that vitamin D can participate in the regulation of more than 2,000 genes in the animal genome through the VDR signaling pathway in animals; almost all tissue cells in the animal body have vitamin D action site genes, among which the immune system is the most abundant, reaching thousands. This shows that vitamin D can achieve all-round regulation of the animal immune system, especially with the rapid expansion of intensive and high-density livestock and poultry farming in recent years. The metabolic pressure on the liver of livestock and poultry is increasing, and liver syndrome is becoming more and more complicated, resulting in vitamin D being unable to be effectively activated through the liver. Its extensive metabolism and immune regulation are severely blocked, so the application of 25-OH-VD3 is receiving more and more attention from the industry.

[0004] The invention patent "A functional additive for improving the reproductive performance of breeding pigs and its preparation method (CN111789192A)" contains chlorophyll iron sodium, yeast selenium, yeast zinc, Cordyceps militaris extract, Epimedium extract, coenzyme Q10, β-carotene, 25-hydroxyvitamin D3, vitamin E, L-carnitine, and polydextrose. It has the functions of increasing the litter size and the number of healthy piglets, enhancing immunity, and significantly improving the reproductive performance of breeding pigs. The effective ingredients are mainly Chinese herbal medicines, not 25-OH-D3. The invention patent "A feed additive for improving the bone strength of sows and the reproductive performance of sows (CN 109820092 A) uses 25-OH-VD3 as one of the vitamin components, and mixes it with composite Chinese herbal medicines, composite organic trace elements, etc. to obtain an additive that improves the bone strength of sows, improves production performance, reduces the elimination rate of sows due to nutritional limb and hoof diseases, and increases the benefits of the pig farming industry.

[0005] At present, the existing technologies mainly consider the calcium and phosphorus metabolism of livestock and poultry, but pay insufficient attention to the relationship between pig reproductive disorders and modern breeding models. It is difficult to systematically improve the comprehensive effects of sows' strong immunity, anti-oxidation, and increased reproduction, thereby making it difficult to improve the reproductive performance of sows. Therefore, it does not meet the existing needs. In this regard, we propose a feed combination for improving the reproductive performance of sows using 25-OH-VD3 and a preparation method thereof. Summary of the invention

[0006] The present invention provides a feed composition for improving the reproductive performance of sows by utilizing 25-OH-VD3 and a preparation method thereof, which has the beneficial effect of improving the reproductive performance of sows and solves the problem mentioned in the above background technology that the prior art mostly considers the calcium and phosphorus metabolism of livestock and poultry, but pays insufficient attention to the relationship between pig reproductive disorders and modern breeding models, and it is difficult to systematically improve the comprehensive effects of sows' strong immunity, anti-oxidation, and increased reproduction, thereby making it difficult to improve the reproductive performance of sows.

[0007] The present invention provides the following technical scheme: a feed composition for improving the reproductive performance of sows by using 25-OH-VD3, wherein the composition comprises the following components by weight: 2-20 parts of 25-hydroxyvitamin D3; 4-20 parts of a complex vitamin mixture; 0.01-1.3 parts of L-carnitine hydrochloride; 0.3-1.6 parts of DL-methionine; 0.1-0.6 parts of L-arginine; 0.01-0.12 parts of L-aspartic acid; 0.2-1.0 parts of L-tryptophan; 0.1-1.0 parts of valine; 0.1-1.3 parts of glycine; 2-8 parts of yeast selenium; 0.01-0.06 parts of chromium picolinate; 10-40 parts of calcium hydrogen phosphate; and 10-40 parts of rice bran powder, which together amount to 100 parts.

[0008] As an optional scheme of the feed combination agent for improving the reproductive performance of sows by using 25-OH-VD3 described in the present invention, the 25-OH-VD3 is a high-purity (≥94%) 25-OH-VD3 diluent with a specification of 0.05% created by the unit of the present invention, which is prepared by dissolving 25-hydroxy-7-dehydrocholesterol in a mixed solvent system comprising a polar solvent and a non-polar solvent, wherein the volume ratio of the polar solvent to the non-polar solvent is 9-4:1, adding an antioxidant, and reacting under light; monitoring the reaction process by high performance liquid chromatography, controlling the raw material conversion rate to be 32-39%, separating and recovering 25-hydroxy-7-dechlorocholesterol after the reaction, and then heating the remaining reactant after separating 25-hydroxy-7-dehydrocholesterol for isomerization, and then freezing for recrystallization to obtain 25-OH-VD3 crystals, and the purity of the 25-OH-VD3 crystals is ≥94%.

[0009] As an optional scheme of the feed combination for improving the reproductive performance of sows by using 25-OH-VD3 described in the present invention, the compound vitamin mixture 4-21 parts comprises: 1-5 parts of 500,000 IU / g vitamin A; 1-5 parts of 50% dl-α-tocopherol acetate; 0.1-0.6 parts of 50% menadione; 0.1-0.4 parts of 98.0% thiamine nitrate; 0.1-1.0 parts of 98.0% pyridoxine hydrochloride; 1-7 parts of 99.0% nicotinamide; 0.05-0.25 parts of 1.0% cyanocobalamin; 0.5-2.0 parts of 2.0% D-biotin; 0.1-0.6 parts of 80% riboflavin; and 0.01-1.6 parts of 10.0% beta-carotene.

[0010] As an optional scheme of the feed composition for improving the reproductive performance of sows by using 25-OH-VD3 described in the present invention, the composition is used in a modern large-scale pig farming model, and 1-2 kg of the composition is added to the basic daily diet of each ton of sows.

[0011] The present invention has the following beneficial effects:

[0012] 1. The feed combination agent for improving the reproductive performance of sows by using 25-OH-VD3 and its preparation method use the independently innovative product 25-OH-VD3 as the main active substance to prepare the combination agent, and feed the sows with the feed added with the combination agent to systematically improve the comprehensive effects of the sows' strong immunity, anti-oxidation, and increased reproduction, thereby effectively improving the reproductive performance of the sows.

[0013] 2. The feed combination agent and preparation method for improving the reproductive performance of sows using 25-OH-VD3 and the innovative 25-OH-VD3 can effectively improve the systemic regulation of pig immunity and metabolism, more comprehensively regulate the metabolism and immune function of the animal body, and thus lay a systematic metabolic and health foundation for improving pig fertility.

[0014] 3. The feed combination agent that utilizes 25-OH-VD3 to improve the reproductive performance of sows and the preparation method thereof strengthen the main effect of 25-OH-VD3. On the one hand, it ensures the direct effect of the feed combination agent with 25-OH-VD3 as the main effect, which strengthens the bones, metabolism, and reproduction of sows; on the other hand, it focuses on the three major health problems of "liver, intestine, and stress" that cause reproductive disorders in intensive pig farming, and specifically focuses on the feed combination agent with 25-OH-VD3 as the main effect. It exerts the three major immune functions of "liver health, intestinal health, and anti-stress", maintains the health of the "hepatointestinal reproductive system" and improves the reproductive capacity of sows, thereby improving the reproductive performance of pigs. BRIEF DESCRIPTION OF THE DRAWINGS

[0015] Figure 1 It is the technical design and implementation diagram of the present invention. DETAILED DESCRIPTION

[0016] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0017] Example 1: This example aims to promote the solution of the problem that the existing technology mainly considers the calcium and phosphorus metabolism of livestock and poultry, but pays insufficient attention to the relationship between pig reproductive disorders and modern breeding models, making it difficult to systematically improve the comprehensive effects of sows' strong immunity, anti-oxidation, and increased reproduction, thereby making it difficult to improve sows' reproductive performance. Please refer to Figure 1 A feed combination for improving the reproductive performance of sows by using 25-OH-VD3. The combination comprises the following components by weight: 5-15 parts of 25-OH-VD3; 5-15 parts of a complex vitamin mixture; 0.02-0.05 parts of L-carnitine hydrochloride; 0.3-0.5 parts of DL-methionine; 0.1-0.3 parts of L-arginine; 0.02-0.07 parts of L-aspartic acid; 0.2-0.6 parts of L-tryptophan; 0.2-0.6 parts of valine; 0.4-0.8 parts of glycine; 2-6 parts of yeast selenium; 0.02-0.05 parts of chromium picolinate; 10-20 parts of calcium hydrogen phosphate; 30-50 parts of rice bran powder, a total of 100 parts.

[0018] The 25-OH-VD3 is a 0.05% 25-OH-VD3 dilution powder created by the present invention; in the multivitamin mixture, the content of vitamin A is 500,000 IU / g; the content of dl-α-tocopheryl acetate is 50%; the content of menadione is 50%; the content of thiamine nitrate is 98.0%; the content of pyridoxine hydrochloride is 98.0%; the content of nicotinamide is 99.0%; the content of cyanocobalamin is 1.0%; the content of D-biotin is 2.0%; the content of riboflavin is 80%; and the content of β-carotene is 10.0%.

[0019] This combination agent is used in modern large-scale pig farming model, with 1-2kg of the combination agent added to every ton of sow feed.

[0020] In this embodiment: the independently innovative product 25-OH-VD3 is used as the main effect active substance to prepare a combination agent, and the feed added with the combination agent is used to feed sows, so as to systematically improve the comprehensive effects of sows' strong immunity, anti-oxidation, and increased reproduction, based on maintaining the health of the sow's "hepatoenteric reproductive system" and improving the sow's fertility, thereby effectively improving the sow's reproductive performance, and solving the problem that the existing technology mostly considers the calcium and phosphorus metabolism of livestock and poultry, but pays insufficient attention to the relationship between pig reproductive disorders and modern breeding models, and it is difficult to systematically improve the comprehensive effects of sows' strong immunity, anti-oxidation, and increased reproduction, thereby It is difficult to improve the reproductive performance of sows.

[0021] Embodiment 2: This embodiment is an optional solution of Embodiment 1. For details, please refer to Figure 1 The combination comprises the following components by weight: 10 parts of 25-OH-VD3; 14 parts of multivitamin mixture; 0.03 parts of L-carnitine hydrochloride; 0.5 parts of DL-methionine; 0.3 parts of L-arginine; 0.05 parts of L-aspartic acid; 0.4 parts of L-tryptophan; 0.4 parts of valine; 0.5 parts of glycine; 5 parts of yeast selenium; 0.04 parts of chromium picolinate; 25 parts of calcium hydrogen phosphate; 39.28 parts of rice bran powder, totaling 100 parts.

[0022] 25-OH-VD3 is a 0.05% 25-OH-VD3 diluted powder prepared by the present invention; the composite vitamin mixture is the same as that in Example 1.

[0023] This combination agent is used in modern large-scale pig farming model, with 1-2kg of the combination agent added to every ton of sow feed.

[0024] Embodiment 3: This embodiment is an optional solution of Embodiment 1. For details, please refer to Figure 1 The combination comprises the following components by weight: 13 parts of 25-OH-VD3; 13 parts of multivitamin mixture; 0.03 parts of L-carnitine hydrochloride; 0.5 parts of DL-methionine; 0.3 parts of L-arginine; 0.05 parts of L-aspartic acid; 0.4 parts of L-tryptophan; 0.4 parts of valine; 0.5 parts of glycine; 5 parts of yeast selenium; 0.04 parts of chromium picolinate; 25 parts of calcium hydrogen phosphate; 39.28 parts of rice bran powder, totaling 100 parts.

[0025] 25-OH-VD3 is a 0.05% 25-OH-VD3 diluted powder prepared by the present invention; the composite vitamin mixture is the same as that in Example 1.

[0026] This combination agent is used in modern large-scale pig farming model, with 1-2kg of the combination agent added to every ton of sow feed.

[0027] Test example: Effect of the combination agent on the reproductive performance of sows.

[0028] 1. Materials and Methods

[0029] 1.1 Test materials: The feed composition used in the feeding test was prepared according to the feed composition preparation methods in Example 1, Example 2 and Example 3 of the present invention.

[0030] 1.2 Experimental animals and diets: 200 weaned sows (Landrace×Large White) with normal reproduction, good health, similar parity (3-5 parities), basically the same breeding date, and similar weight were selected and randomly divided into 4 groups, including control group, experimental group 1, experimental group 2, and experimental group 3. Each group had 50 sows, raised in a single pen, and 50 replicates in each group; the piglets born by the experimental sows were tested together, and the piglets were weaned at 21 days. The experimental sows entered the formal period after the piglets were weaned, and the trial period lasted for 135 days until weaning 3 weeks after delivery. The feeding management and immunization procedures of pregnant sows, lactating sows and lactating piglets during the experimental period were carried out according to normal operating procedures.

[0031] The basal diet was a corn-soybean meal diet, and the feed was formulated with reference to the NRC recommended nutrient levels for sows. See Table 1 for details, and the experimental design is shown in Table 2.

[0032] Table 1 Basic diet and nutritional level

[0033] Raw material composition content% Nutrition level corn 61.20 Digestible energy (MJ / kg) 13.65 Soybean meal 19.80 Crude protein (%) 15.90 Bran 8.00 calcium(%) 1.09 Soybean Oil 3.50 Total phosphorus (%) 0.75 Rice bran 2.00 Lysine(%) 0.82 Premix 4.00 Methionine + Cystine (%) 0.64 total 100.00

[0034] Table 2 Dietary feeding design for the experimental groups

[0035]

[0036]

[0037] 1.3 Determination of economic indicators

[0038] (1) Sow production performance indicators: average daily feed intake, average piglet size, number of piglets born alive, number of stillbirths and live piglet rate;

[0039] (2) Piglet growth performance indicators: piglet birth weight, weaning piglet weight, and average daily weight gain.

[0040] 1.4 Determination of antioxidant and immune indicators

[0041] On the day of farrowing, 5 sows were randomly selected from each group for blood collection from the ear vein (10 mL). The blood samples were kept at room temperature for 10 min and centrifuged at 3000 r / min for 15 min to prepare serum. The serum was transferred to Eppendorf tubes and stored at -20°C.

[0042] Total superoxide dismutase (T-SOD) was measured using the Fe 3+ reduction method; total antioxidant capacity (T-AOC) was measured using the xanthine oxidase method; glutathione peroxidase (GSH-Px) was measured using the dithiodinitrobenzoic acid method; catalase (CAT) was measured using the ammonium molybdate colorimetric method; and enzyme-linked immunosorbent assay (ELISA) was used to detect immunoglobulin G (IgG), immunoglobulin A (IgA), and immunoglobulin M (IgM) levels. All of the above indicators were measured using kits from Nanjing Jiancheng Bioengineering Institute, and the specific operations were carried out according to the instructions.

[0043] The statistical data were analyzed using conventional difference test methods.

[0044] 2. Results and Analysis

[0045] 2.1 Effect of the Combination of the Present Invention on Reproductive Performance of Sows

[0046] Table 3 Effect of the additive of the present invention on sow reproductive performance

[0047]

[0048]

[0049] As can be seen from Table 3, the feed combination of the present invention can significantly improve the reproductive performance of sows, increase the average number of piglets born per litter, the number of live piglets, and the number of healthy piglets per sow, and reduce the number of stillbirths per pig.

[0050] 2.2 Effect of the combination of the present invention on the production performance of suckling piglets

[0051] Table 4 Effect of the additive of the present invention on the production performance of suckling piglets

[0052]

[0053] As can be seen from Table 4, the feed combination of the present invention can significantly improve the production performance of suckling piglets, increase the birth weight, weaning weight, average daily weight gain and weaning piglet survival rate of piglets, and increase the production efficiency of pig farming.

[0054] 2.3 Effect of the additive of the present invention on the antioxidant function of sows

[0055] Table 5 Effect of the additives of the present invention on the antioxidant function of sows

[0056]

[0057]

[0058] As can be seen from Table 5, the feed combination fortified with 25-OH-VD3 for improving the reproductive capacity of sows can significantly improve the antioxidant function of sows, increase the levels of serum total antioxidant capacity, superoxide dismutase, glutathione peroxidase, catalase, etc., and reduce the level of serum malondialdehyde.

[0059] 2.4 Effect of the Combination of the Present Invention on the Immune Function of Sows

[0060] Table 6 Effect of the additive of the present invention on the immune function of sows

[0061]

[0062] It can be seen from Table 6 that the feed combination of the present invention can significantly improve the immune function of sows, increase the levels of serum immunoglobulin A, immunoglobulin G, and immunoglobulin M, and enhance the immunity of sows.

[0063] 3. Conclusions of the experiment and production application of the present invention

[0064] The combination of the present invention uses high-quality 25-OH-VD3 independently developed by the inventor as the main active substance, and is specifically used for healthy and efficient sow breeding production practice in intensive pig farming mode. Comparative experiments and production application practices show that the feed combination of the present invention can not only significantly improve the reproductive performance of sows, but also significantly improve the immunity, anti-stress and antioxidant capacity of sows, and improve the health and production status of sows.

[0065] The above is only one of the preferred embodiments of the present invention. For technicians in the field of pig farming, based on the technical principles of the present invention and combined with the specific reality of their own enterprises, especially the physical condition and health of breeding sows, they can make several further optimizations and improvements to improve the technical effects of the practical application of the present invention.

[0066] It should be noted that, in this article, relational terms such as first and second, etc. are only used to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Moreover, the terms "include", "comprise" or any other variants thereof are intended to cover non-exclusive inclusion, so that a process, method, article or device including a series of elements includes not only those elements, but also other elements not explicitly listed, or also includes elements inherent to such process, method, article or device.

[0067] The above is only a preferred embodiment of the present invention. It should be pointed out that for ordinary technicians in this technical field, several improvements and modifications can be made without departing from the technical principles of the present invention. These improvements and modifications should also be regarded as the scope of protection of the present invention.

Claims

1. A feed composition for improving the reproductive performance of sows using 25-OH-VD3, characterized in that: The combination comprises 25-OH-VD3, a multivitamin mixture, L-carnitine hydrochloride, DL-methionine, L-arginine, L-aspartic acid, L-tryptophan, valine, glycine, yeast selenium, chromium picolinate, and calcium hydrogen phosphate.

2. The feed composition for improving the reproductive performance of sows by using 25-OH-VD3 according to claim 1, characterized in that: The 25-OH-VD3 is a 25-OH-VD3 diluent created by the present invention, with a purity of not less than 94% and a specification of 0.05%.

3. The feed composition for improving the reproductive performance of sows by using 25-OH-VD3 according to claim 1, characterized in that: The composite vitamin mixture comprises 1-5 parts of 500,000 IU / g vitamin A, 1-5 parts of 50% dl-α-tocopherol acetate, and 0.1-0.6 parts of 50% menadione. 98.0% thiamine nitrate 0.1-0.4 parts; 98.0% pyridoxine hydrochloride 0.1-1.0 parts; 99.0% nicotinamide 1-7 parts; 1.0% cyanocobalamin 0.05-0.25 parts; 2.0% D-biotin 0.5-2.0 parts; 80% riboflavin 0.1-0.6 parts; 10.0% beta-carotene 0.01-1.6 parts.

4. The feed composition for improving the reproductive performance of sows by using 25-OH-VD3 according to claim 1, characterized in that: The combination is used in modern large-scale pig farming mode, with 1-2 kg of the combination added to the basic diet of each ton of sows.

5. A method for preparing a feed composition for improving the reproductive performance of sows by using 25-OH-VD3, used for the feed composition for improving the reproductive performance of sows by using 25-OH-VD3 as claimed in claim 1, characterized in that: The composition comprises the following components by weight: 2-20 parts of 25-OH-VD3; 4-21 parts of multivitamin mixture; 0.01-1.3 parts of L-carnitine hydrochloride; 0.3-1.6 parts of DL-methionine; 0.1-0.6 parts of L-arginine, 0.01-0.12 parts of L-aspartic acid, 0.2-1.0 parts of L-tryptophan, 0.1-1.0 parts of valine, 0.1-1.3 parts of glycine, 2-8 parts of yeast selenium, 0.01-0.06 parts of chromium picolinate, 10-40 parts of calcium hydrogen phosphate, and 10-40 parts of rice bran powder, a total of 100 parts, the above components are stirred and mixed to prepare a combination agent.

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