Calcium-containing tissue strengthening agents and use thereof

a calcium-containing tissue and agent technology, applied in the direction of biocide, heterocyclic compound active ingredients, drug compositions, etc., can solve the problems of affecting so as to increase bone mass, and accelerate the deposition of calcium

Inactive Publication Date: 2005-09-29
HAYASHIBARA BIOCHEMICAL LAB INC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

[0005] The present inventors eagerly screened plant substances, leading to an unexpected finding that a series of compounds having the basic structure represented by any one of General Formulae 1 to 5 as described heretoafter and their precursors (referred to as “compounds of General Formulae 1 to 5” hereinafter) consistently leads the calcium metabolism system in animals towards calcium deposition to accelerate the depositi

Problems solved by technology

Aging usually weakens calcium-containing tissues such as bone and tooth.
Such a balance may be lost by a variety of causes such as unbalanced hormone secretion which is caused by extreme diet and aging, resulting in an excessive bone absorption.
The way of supplementing calcium however should be carefully chosen because calcium intake may be ineffective due to its less assimilativity, as well as because an ex

Method used

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  • Calcium-containing tissue strengthening agents and use thereof
  • Calcium-containing tissue strengthening agents and use thereof
  • Calcium-containing tissue strengthening agents and use thereof

Examples

Experimental program
Comparison scheme
Effect test

experiment 1

Searching from Variety of Organic Compounds by Measuring Alkaline Phosphatase Activity

[0039] To increase number of osteoblastic cells having calcium-deposition activity is important for increasing bone mass. The compounds inducing the differentiation of mouse pre-osteoblastic cell MC3T3-E1 (RCB1126, Riken Gene Bank, Tokyo, Japan) were searched from variety of organic compounds known as plant ingredients by measuring alkaline phosphatase activity. The activity is at low level in pre-osteoblastic cells and at high level in osteoblastic cells differentiated form the pre-osteoblastic cells.

Culturing Mouse Pre-Osteoblastic Cells

[0040] Mouse pre-osteoblastic cell line MC3T3-E1 was suspended in α-MEM medium (pH6.8) supplemented with 10 mM HEPES and 10(v / v) % calf serum albumin (produced by Gibco BRL Company), which contains the ingredients listed in Table 7 to be concentration of 5×104 cells / ml, placed into 24 hole plate (Commercialized by Falcon Becton Dickinson and Company) by each ...

experiment 2

Selecting from Variety of Organic Compounds by Measuring Calcium Deposition Activity

[0043] Following Experiment 1 which investigates differentiating pre-osteoblastic cells into osteoblastic cells, some of organic compounds were investigated whether they actually enhance calcium deposition or not. Indomethacin (commercialized by Funakoshi, Co., Ltd., Tokyo, Japan) (positive control), kaempferol (commercialized by Funakoshi, Co., Ltd., Tokyo, Japan), hesperetin (commercialized by Funakoshi, Co., Ltd., Tokyo, Japan), gallic acid (commercialized by Nacalai Tesque, Kyoto, Japan), and coffeic acid (commercialized by Wako Pure Chemical Industries, Osaka, Japan) were selected as organic compounds. Mouse MC3T3-E1 cells were treated with 20 mM each above compound dissolved in 1 ml of α-MEM medium supplemented with 10% (v / v) fetal calf serum and containing 10 mM β-glycerophosphate. Since final ethanol concentration in the medium is 0.2% (v / v), α-MEM medium containing 0.2% (v / v) ethanol was p...

experiment 3

Comparing Between Flavonoids

[0046] Other flavonoid compounds than kaempferol or hesperetin were investigated where they have the same effect or not. Flavone (commercialized by Kanto Kagaku, Tokyo, Japan), apigenin (commercialized by Funakoshi, Co., Ltd., Tokyo, Japan), flavonol (commercialized by Tokyo Kasei Kogyo, Co., Ltd., Tokyo, Japan), quercetin (commercialized by Kanto Kagaku, Tokyo, Japan), kaempferol (commercialized by Funakoshi, Co., Ltd., Tokyo, Japan), rutin (commercialized by Kanto Kagaku, Tokyo, Japan), flavanone (commercialized by Kanto Kagaku, Tokyo, Japan), naringenin (commercialized by Ardrich Corporation), hesperetin (commercialized by Funakoshi, Co., Ltd., Tokyo, Japan), hesperidin (commercialized by Kanto Kagaku, Tokyo, Japan), chalcone (commercialized by Merk Corporation), fuloretin (commercialized by Sigma Corporation), catechin (commercialized by Sigma Corporation), ipriflavone (commercialized by Daito Corporation, Toyama, Japan), taxifolin (commercialized b...

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Abstract

The present invention has an object to provide an agent for strengthening calcium-containing tissues, which can be safely applied; and its use: The present invention solves the object by providing an agent for strengthening calcium-containing tissues, which comprises one or more flavones, flavonols, flavanones, flavanonols, anthocyanidins, flavanols, chalcones, and aurones.

Description

TECHNICAL FIELD [0001] The present invention relates to an agent for strengthening calcium-containing tissues, which is usable to increase calcium contents in tissues such as bone and tooth, as well as to its use. BACKGROUND ART [0002] Aging usually weakens calcium-containing tissues such as bone and tooth. Bone tissues undergo without cease the formation and absorption of bone which are kept in an adequate balance in younger living bodies. Such a balance may be lost by a variety of causes such as unbalanced hormone secretion which is caused by extreme diet and aging, resulting in an excessive bone absorption. If such a state prolongs, then calcium-containing tissues such as bone and tooth loose calcium, one of their important elements, and this may accelerate the incidence of bone disorders such as osteoporosis, bone break and backache, as well as of tooth disorders such as dental caries and periodontosis. [0003] Calcium in living bodies is present in a calcium phosphate solid such...

Claims

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Application Information

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IPC IPC(8): A23L1/30A23L1/304A23L33/15A61K8/49A61K31/12A61K31/343A61K31/352A61P19/08A61P19/10A61P43/00A61Q11/00C07C49/796C07C49/83C07C49/835C07D307/83C07D311/30C07D311/32C07D311/62
CPCA23L1/3002C07D311/62A23L1/302A23L1/304A61K8/4973A61K8/498A61K31/12A61K31/343A61K31/352A61Q11/00C07C49/796C07C49/83C07C49/835C07D307/83C07D311/30C07D311/32A23L1/3004A23L33/105A23L33/11A23L33/15A23L33/16A61P19/08A61P19/10A61P43/00
Inventor MIYAKE, MASAKIUSHIO, SHIMPEIIWAKI, KANSOKURIMOTO, MASASHI
Owner HAYASHIBARA BIOCHEMICAL LAB INC
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