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Artemisia selengensis straw extractive and preparation method and application thereof

A technology of extracts and straws, applied in the fields of functional food and phytochemicals, can solve the problems of large sample loss, high cost, difficult industrial production, etc., and achieve the effect of inhibiting the formation of AGEs

Inactive Publication Date: 2013-07-24
NANJING NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The purification process for monomeric compounds is too cumbersome, and the sample loss is also large (resulting in low yield); in addition, a large amount of solvent elution will also greatly increase the cost; and the separation and purification of silica gel column chromatography and Sephadex LH-20 chromatographic column are difficult to industrialize Production

Method used

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  • Artemisia selengensis straw extractive and preparation method and application thereof
  • Artemisia selengensis straw extractive and preparation method and application thereof
  • Artemisia selengensis straw extractive and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Example 1: Artemisia stalks were dried and crushed, sieved through 20 mesh, weighed 500g into the reactor, added 75% ethanol (volume ratio) 7500mL, heated in a water bath at 90°C, stirred and refluxed for 40min, filtered, and the extract was collected , The residue was extracted again under the same conditions, the two extracts were combined, and concentrated under reduced pressure to remove ethanol. Extract twice with 3 times the volume of petroleum ether (n-hexane), combine the extracted parts, and concentrate under reduced pressure. The concentrated solution is further purified with macroporous adsorption resin AB-8, and water, 10%, 30%, 50%, and 70% are sequentially Ethanol elution, 95% ethanol elution chromatography column. The ethanol-eluted fractions were collected, concentrated under reduced pressure, and dried in vacuo to obtain solid powder. Determination of the total flavonoid content of each eluted fraction to the solid content is shown in Table 1 below.

...

Embodiment 2

[0037] Example 2: Efficacy Test for Inhibiting Protein Glycosylation

[0038] 1. Kinetic study of MGO on the formation of AGEs in BSA-MGO system

[0039]Dissolve BSA with a phosphate buffer solution with a concentration of 0.2mM and pH=7.4, add 0.2mL of a mixed solution of penicillin and streptomycin and 3mL of a 3mg / mL BSA diluent into a 10mL centrifuge tube, and then add diluted MGO solution 3mL, so that the final concentrations were 0.1, 0.25, 0.5, 1.5mM, respectively, heated in a water bath at 37°C, and 500μL samples were taken at 0, 4, 8, 12, 24, 72, 144, 288, 432, and 720h respectively. 2mL centrifuge tube, stored at -20°C. After thawing, measure the fluorescence value of Ex / Em=340nm / 465nm, set Gain to 55, use phosphate buffer saline (PBS) instead of MGO solution as blank, and do three parallel experiments.

[0040] 2. Kinetic study of GO on the formation of AGEs in BSA-GO system

[0041] Dissolve BSA with a phosphate buffer solution with a concentration of 0.2mM and ...

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Abstract

The invention relates to an artemisia selengensis straw extractive, a preparation method and a function of inhibiting protein glycosylation. The preparation method of the extractive comprises the following steps of: by taking the artemisia selengensis straw as the raw material, carrying out reflux extraction by using 75% of ethanol with the solid-liquid ratio of 1:15 at 90 DEG C, filtering, carrying out decompression concentration on the filtrate, extracting and decoloring with petroleum ether, further purifying macroporous resin AB-8 column chromatography, eluting the chromatography column by 10% of water, 30-50% of water and 95% of ethanol, collecting 30-50% of eluted component of ethanol for decompression concentration and carrying out vacuum drying to obtain solid powder. The total flavone content of the artemisia selengensis extractive is not less than 80% and the main components comprise rutin and luteolin; the artemisia selengensis straw extractive has the activities of capturing active dicarbonyl compound (methylglyoxal MGO and glyoxal GO) and inhibiting the protein glycosylation (AGEs).

Description

technical field [0001] The patent of this invention relates to the fields of functional food and phytochemistry, the separation and preparation of natural active factors and their application in functional food and health products. Background technique [0002] Advanced glycation end products (Advance glycation end products, AGEs) are under non-enzymatic conditions, the free amino groups of proteins, amino acids, lipids or nucleic acids and the aldehyde groups of reducing sugars undergo condensation, rearrangement, cleavage, oxidation A set of stable end products produced after modification. Active dicarbonyl compounds such as methylglyoxal (MGO) and diacetaldehyde (GO) produced by lipid peroxidation, glycolysis, and enzymatic reactions in the human body can quickly react with amino groups of proteins to form AGEs. Under normal circumstances, AGEs in the body are maintained in a relatively balanced state, but when excessive endogenous body production or exogenous intake of ...

Claims

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Application Information

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IPC IPC(8): A23L1/212A23L1/29A61K36/899A61P3/10A61K135/00A23L19/00A23L33/00
Inventor 吕丽爽邓蓉华李晓明
Owner NANJING NORMAL UNIVERSITY
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