Method for prediabetes screening
A pre-diabetes and detection method technology, applied in the direction of disease diagnosis, biological testing, measuring devices, etc., can solve the problems of delaying treatment, not implying pre-diabetes, etc., achieve simple detection, prevent the development of diabetes or complications, and reduce symptoms The effect of treatment costs
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Embodiment 1
[0172] In this example, a method for producing prediabetic mice will be described.
[0173] 5-week-old C57BL / 6J (hereinafter abbreviated as "B6 mice") were purchased from Japan Charles River Co., Ltd. After the animals arrived, they were brought into the animal breeding room, and under a 12-hour light-dark cycle, they were reared until the target week in an environment where they could freely ingest experimental animal solid feed (Oriental Yeast Co., Ltd.) as feed and tap water as drinking water. age, used for experiments.
Embodiment 2
[0175] The mice were intraperitoneally administered 1 mL / kg body weight of pentobarbital sodium injection for adequate anesthesia, and after body weight was measured, blood was collected from the heart with a heparinized syringe. 1,000 g of the obtained blood was centrifuged at 4° C. for 10 minutes to obtain a plasma sample. Visceral fat was harvested after blood collection and mice were euthanized. The amount of visceral fat collected was measured, and the results were expressed in image 3 middle.
Embodiment 3
[0177] The measurement of the blood glucose level was carried out using a small blood glucose measuring device (glutest sensor, Sanwa Chemical Laboratory Co., Ltd.). The measurement of the insulin level in the blood was carried out using an ultra-high sensitivity mouse insulin measurement kit (Mori Naga Institute of Science). Blood glucose level and blood insulin measurement results are respectively expressed in figure 1 with figure 2 middle.
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