Schizothoracinae otolith slice production method

A production method and otolith technology, which is applied in the field of production of fish otolith slices, can solve the problems of complicated operation, unsuitable operation, and long time consumption

Inactive Publication Date: 2015-02-25
HEILONGJIANG RIVER FISHERY RES INST CHINESE ACADEMY OF FISHERIES SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to solve the problems that existing methods for making schizothoracic otolith slices use single material

Method used

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  • Schizothoracinae otolith slice production method
  • Schizothoracinae otolith slice production method
  • Schizothoracinae otolith slice production method

Examples

Experimental program
Comparison scheme
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specific Embodiment approach 1

[0018] Embodiment one: a kind of preparation method of Schizothorax otolith slices is specifically completed according to the following steps:

[0019] 1. Extract otoliths: Use a scalpel and curved tweezers to remove the otoliths of Schizothorax, and then remove the dirt on the surface of the Schizothorax otoliths;

[0020] 2. Soak otoliths: put the schizothoracic otoliths from which the surface dirt has been removed in a 2mL-5mL centrifuge tube, and then add alcohol with a volume fraction of 95% to soak the schizothoracic otoliths for 2d-5d to obtain the alcohol-soaked otoliths. Schizothorax otoliths;

[0021] 3. Cleaning the otoliths: use distilled water to rinse the surface of the schizothoracic otoliths soaked in alcohol for 2 to 3 times, and then dry them at room temperature to obtain the dried schizothoracic otoliths;

[0022] 4. Fix otoliths: First, apply a layer of release agent on the bottom and side walls of the plastic mold, and then place the dried Schizothorax ot...

specific Embodiment approach 2

[0030] Specific embodiment 2: The difference between this embodiment and specific embodiment 1 is: the preparation method of the resin described in step 4 is as follows: mix epoxy resin and hardener, and stir at a stirring speed of 50r / min~60r / min 2min to 3min, and then stand still for 2min to 3min to obtain the resin; the mass ratio of the epoxy resin to the hardener is 25:3. Other steps are the same as in the first embodiment.

[0031] The epoxy resin described in this embodiment purchases the EpoFix resin produced in Struers (Struers), product number 40200029; Described hardener is the EpoFix that Struers produces and is used in conjunction with EpoFix resin Hardener, product number is also 40200029.

specific Embodiment approach 3

[0032] Specific embodiment three: the difference between this embodiment and specific embodiment one or two is: in step two, place the schizothoracid otoliths from which the surface dirt has been removed in a 2mL~3mL centrifuge tube, and then add a volume fraction of 95 The otoliths of Schizothorax were soaked in 100% alcohol for 2d-3d to obtain the otoliths of Schizothorax soaked in alcohol. Other steps are the same as those in Embodiment 1 or 2.

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Abstract

The invention discloses a Schizothoracinae otolith slice production method, and relates to a fish otolith slice production method. A purpose of the present invention is to solve problems of single material use, complex operation, time consuming and difficult operation of the existing Schizothoracinae otolith slice production method. The production method of the present invention comprises: 1, removing otolith, 2, soaking the otolith, 3, washing the otolith, 4, fixing the otolith, 5, grinding the slice, and 6,sealing the slice to obtain the clear Schizothoracinae otolith slice capable of being stored for a long time. According to the present invention, the Schizothoracinae otolith slice production method is provided.

Description

technical field [0001] The invention relates to a method for making fish otolith slices. Background technique [0002] Fish otoliths exist in the labyrinth of the eardrum in fish. There are three pairs in total. They are mainly composed of carbonate minerals and a small amount of organic matter and trace elements. Physiologically, they play the role of hearing and balance. Otoliths have attracted the attention of fish researchers very early, and they were originally used as an effective way of age identification materials, which are more accurate and easy to read than the annual rings on scales. Since Pannella first discovered the solar disc in 1971, there have been a lot of reports on various related studies using the solar disc, and otoliths have once become a research hotspot. [0003] As a living mineral, otolith has both the excellent characteristics of living organisms and minerals. It not only records the relevant information in the growth and life history of fish, ...

Claims

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Application Information

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IPC IPC(8): G01N1/28G01N1/36B24B1/00
Inventor 王继隆马波霍堂斌
Owner HEILONGJIANG RIVER FISHERY RES INST CHINESE ACADEMY OF FISHERIES SCI
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