Method for manufacturing section of xenophysogobio boulengeri otolith
A production method, the technology of the different swimbladder mahi, which is applied in the field of fish age identification, can solve the problems of ring pattern defect or fragmentation, affect the interpretation of annual rings, and the scales are easy to fall off, and achieve the effect of high accuracy
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Embodiment 1
[0021] ①Picking: Use scissors and tweezers to remove the otoliths from the balance sac in the cochlea of Mahiba maacha, and remove impurities such as the cochlea;
[0022] ② Soaking: Put the otoliths with the cochlea and other impurities removed into a 5ml EP tube, add 95% alcohol and soak the otoliths in 95% alcohol for 5 days;
[0023] ③Fixation: Take out the soaked otoliths and place them on a glass slide to dry, and drop 1 drop of neutral gum to cover the otoliths and fix them;
[0024] ④ Baking: Put the fixed otolith slides into an oven and bake at 33°C for 30 hours;
[0025] ⑤ Cooling: After the otoliths on the glass slide are baked, take them out and place them in a ventilated place for 7 hours, then cool and dry;
[0026] ⑥ Grinding sheet: Use No. 3000 sandpaper to grind the otolith along the water, observe while grinding, and after grinding to the center of the otolith, melt the neutral gum with an alcohol lamp, then turn the other side of the otolith up with a nee...
Embodiment 2
[0030] ①Picking: Use scissors and tweezers to remove the otoliths from the balance sac in the cochlea of Mahiba maacha, and remove impurities such as the cochlea;
[0031] ② Soaking: Put the otoliths with the cochlea and other impurities removed into a 10ml EP tube, add 96% alcohol and soak the otoliths in 96% alcohol for 4 days;
[0032] ③Fixation: Take out the soaked otoliths and place them on a glass slide to dry, and drop 2 drops of neutral gum to cover the otoliths and fix them;
[0033] ④ Baking: put the fixed otolith slides into an oven and bake at 35°C for 25 hours;
[0034] ⑤ Cooling: After the otoliths on the glass slide are baked, take them out and place them in a ventilated place for 6 hours, then cool and dry;
[0035] ⑥ Grinding sheet: use 5000 sandpaper to polish the otolith along the water, observe while grinding, and after grinding to the center of the otolith, melt the neutral gum with an alcohol lamp, then turn the other side of the otolith up with a need...
Embodiment 3
[0039] ①Picking: Use scissors and tweezers to remove the otoliths from the balance sac in the cochlea of Mahiba maacha, and remove impurities such as the cochlea;
[0040] ② Soaking: Put the otoliths with the cochlea and other impurities removed into a 5ml EP tube, add 98% alcohol and soak the otoliths in 98% alcohol for 3 days;
[0041] ③Fixation: Take out the soaked otoliths and place them on a glass slide to dry, and drop 2 drops of neutral gum to cover the otoliths and fix them;
[0042] ④ Baking: put the fixed otolith slides into an oven and bake at 38°C for 20 hours;
[0043] ⑤ Cooling: After the otoliths on the glass slide are baked, take them out and place them in a ventilated place for 5 hours, then cool and dry;
[0044] ⑥ Grinding sheet: Use No. 3000 sandpaper to grind the otolith along the water, observe while grinding, and after grinding to the center of the otolith, melt the neutral gum with an alcohol lamp, then turn the other side of the otolith up with a ne...
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