Method for manufacturing section of xenophysogobio boulengeri otolith

A production method, the technology of the different swimbladder mahi, which is applied in the field of fish age identification, can solve the problems of ring pattern defect or fragmentation, affect the interpretation of annual rings, and the scales are easy to fall off, and achieve the effect of high accuracy

Inactive Publication Date: 2014-01-22
YANGTZE RIVER FISHERIES RES INST CHINESE ACAD OF FISHERY SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Scales are usually used to identify the age of the genus genus, but the external scales of the genus are very container and are often washed by the water flow, the scales are easy to fall off and form secondary scales, and the ring patterns made of the scales are defective or broken, which affects the age. interpretation of the wheel
Therefore, the age data of the scales used to identify the genus is inaccurate

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] ①Picking: Use scissors and tweezers to remove the otoliths from the balance sac in the cochlea of ​​Mahiba maacha, and remove impurities such as the cochlea;

[0022] ② Soaking: Put the otoliths with the cochlea and other impurities removed into a 5ml EP tube, add 95% alcohol and soak the otoliths in 95% alcohol for 5 days;

[0023] ③Fixation: Take out the soaked otoliths and place them on a glass slide to dry, and drop 1 drop of neutral gum to cover the otoliths and fix them;

[0024] ④ Baking: Put the fixed otolith slides into an oven and bake at 33°C for 30 hours;

[0025] ⑤ Cooling: After the otoliths on the glass slide are baked, take them out and place them in a ventilated place for 7 hours, then cool and dry;

[0026] ⑥ Grinding sheet: Use No. 3000 sandpaper to grind the otolith along the water, observe while grinding, and after grinding to the center of the otolith, melt the neutral gum with an alcohol lamp, then turn the other side of the otolith up with a nee...

Embodiment 2

[0030] ①Picking: Use scissors and tweezers to remove the otoliths from the balance sac in the cochlea of ​​Mahiba maacha, and remove impurities such as the cochlea;

[0031] ② Soaking: Put the otoliths with the cochlea and other impurities removed into a 10ml EP tube, add 96% alcohol and soak the otoliths in 96% alcohol for 4 days;

[0032] ③Fixation: Take out the soaked otoliths and place them on a glass slide to dry, and drop 2 drops of neutral gum to cover the otoliths and fix them;

[0033] ④ Baking: put the fixed otolith slides into an oven and bake at 35°C for 25 hours;

[0034] ⑤ Cooling: After the otoliths on the glass slide are baked, take them out and place them in a ventilated place for 6 hours, then cool and dry;

[0035] ⑥ Grinding sheet: use 5000 sandpaper to polish the otolith along the water, observe while grinding, and after grinding to the center of the otolith, melt the neutral gum with an alcohol lamp, then turn the other side of the otolith up with a need...

Embodiment 3

[0039] ①Picking: Use scissors and tweezers to remove the otoliths from the balance sac in the cochlea of ​​Mahiba maacha, and remove impurities such as the cochlea;

[0040] ② Soaking: Put the otoliths with the cochlea and other impurities removed into a 5ml EP tube, add 98% alcohol and soak the otoliths in 98% alcohol for 3 days;

[0041] ③Fixation: Take out the soaked otoliths and place them on a glass slide to dry, and drop 2 drops of neutral gum to cover the otoliths and fix them;

[0042] ④ Baking: put the fixed otolith slides into an oven and bake at 38°C for 20 hours;

[0043] ⑤ Cooling: After the otoliths on the glass slide are baked, take them out and place them in a ventilated place for 5 hours, then cool and dry;

[0044] ⑥ Grinding sheet: Use No. 3000 sandpaper to grind the otolith along the water, observe while grinding, and after grinding to the center of the otolith, melt the neutral gum with an alcohol lamp, then turn the other side of the otolith up with a ne...

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PUM

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Abstract

The invention relates to a method for manufacturing a section of a xenophysogobio boulengeri otolith for fish age determination. The otolith of xenophysogobio boulengeri is picked, soaked, fixed, baked, cooled, grinded, degummed and mounted, clear annulations appear on the otolith of the xenophysogobio boulengeri, and therefore the age of the xenophysogobio boulengeri is determined. The new method is provided for fish age study, and is suitable for the study of the age of teleostean with two sides of the otoliths thick.

Description

technical field [0001] The present invention relates to identification of fish age. Background technique [0002] Heterophyllus genus belongs to Cypriniformes Cyprinidae Cyprinidae subfamily Heterophyllus genus. It is a unique fish in the upper reaches of the Yangtze River. It is mainly distributed in the upper reaches of the Yangtze River and its tributaries. It usually inhabits on the sandy bottom of rivers and rivers. Feeds on benthic invertebrates. Due to the establishment of the Three Gorges Dam on the Yangtze River, the ecological environment in the upper reaches of the Yangtze River has undergone major changes, and the unique fish species such as echinococcidae have gradually decreased. In order to protect this unique fish, it is very necessary to conduct age research on this unique fish, which is of great significance to the resource management and protection of Isomahicus. [0003] At present, the commonly used materials for fish age identification include scales,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K61/00
CPCY02A40/81
Inventor 段辛斌王生陈大庆刘绍平陈永柏田辉伍刘明典王珂汪登强
Owner YANGTZE RIVER FISHERIES RES INST CHINESE ACAD OF FISHERY SCI
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