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Method for researching migratory behaviour of anguilla japonica by employing otolith microchemistry

A microchemical and otolithic technology, applied in the field of fish ecology, achieves low radiation dose, safe operation and sensitive detection

Inactive Publication Date: 2012-09-26
SHANGHAI OCEAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
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Problems solved by technology

However, there are still many gaps in its migratory habits and the location of its growth habitat.

Method used

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  • Method for researching migratory behaviour of anguilla japonica by employing otolith microchemistry
  • Method for researching migratory behaviour of anguilla japonica by employing otolith microchemistry
  • Method for researching migratory behaviour of anguilla japonica by employing otolith microchemistry

Examples

Experimental program
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Embodiment

[0027] Preparation of Japanese eel otolith cross-section:

[0028] Excavation: In order to avoid elemental contamination, a pair of sagittal otoliths were taken out from the ear capsule of Japanese eel with clean bamboo tweezers. In an ultrasonic cleaner, the surface film and mucus of the otoliths were washed with deionized water, and placed in the numbered Dry in the centrifuge tube;

[0029] Embedding: Number the abrasive tools, and the number is consistent with the label of the centrifuge tube. Select the left otolith as the analysis material, place the concave side of the otolith upward, put it horizontally into a plastic otolith abrasive tool (the specifications of the abrasive tool are: length 22mm, width 19mm, height 8mm), and slowly pour the prepared cold embedding resin for embedding , let stand until hardened, figure 1 Shown is a schematic diagram of the shape of Japanese eel otolith and the grinding direction after embedding. The arrow in the figure shows the dire...

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Abstract

The invention discloses a method for researching migratory behaviour of anguilla japonica by employing otolith microchemistry, wherein the migratory behaviour of anguilla japonica is determined by analyzing the microchemical constitution of the cross section of otolith life history of anguilla japonica by using a proton scanning microprobe technique, and the process comprises otolith excavating, embedding, flaking, film bonding, otolith centering, life history cross section scanning, guide sample correction and life history analysis. The invention provides a method which is capable of accurately positioning the center of otolith, sensitive in detection and quick in analyzing Sr / Ca ratio change in the cross section of the otolith life history, so that the following problems are solved: low Sr element content in otolith, long detection time, big errors, difficulty in accurate positioning because the center of an otolith ground slice of anguilla japonica is not at the geometric center, easiness in loss of information of partial stages of the life history caused by the use of a quick line scanning manner, and the like; and in combination with the annual ring marks of the otolith, the migratory behaviour of anguilla japonica at habitats such as fresh water, river mouths, oceans and the like can be effectively analyzed.

Description

technical field [0001] The invention relates to a method for analyzing the microchemical composition of the cross-section of the Japanese eel otolith life history by using a proton scanning microprobe technology (abbreviated as PIXE), and combining the annual ring marks to determine the migration behavior in its life history, which belongs to fish ecology field. Background technique [0002] The Japanese eel (Anguilla japonica) is a migratory fish that is widely distributed in the waters of East Asian countries such as Taiwan, China, South Korea, and Japan. Its spawning grounds have been confirmed to be located on Mariana Island thousands of kilometers away from the freshwater rivers where it is fattened Western waters (14°N, 142-143°E). However, there are still many gaps in its migratory habits and the location of its growth habitat. my country is the world's largest eel-producing country. Among the world's eel breeding production (about 200,000 tons) in recent years, the...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K61/00
CPCY02A40/81
Inventor 郭弘艺唐文乔郑怡沈皓魏凯李辉华刘东张亚张旭光
Owner SHANGHAI OCEAN UNIV
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