Method for acquiring clean long-placed fish otolith
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A fish otolith cleaning technology, applied in the field of fish otolith acquisition, can solve problems such as difficult removal, detection interference, and a large amount of dirt in the otolith, achieving obvious anatomical effects and improving work efficiency
Inactive Publication Date: 2014-12-10
HEILONGJIANG RIVER FISHERY RES INST CHINESE ACADEMY OF FISHERIES SCI
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[0005] The present invention aims to solve the problem that in the prior art, the otoliths collected from long-existing fish will be more or less covered with some tissue and mucus, and the otoliths will show a fluffy state after drying quickly, which will bring great benefits for subsequent detection. Great interference, the otoliths collected from rotten fish may even contain a lot of dirt, and it is difficult to remove the problem, and provide a method to obtain clean long-term fish otoliths
Method used
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specific Embodiment approach 1
[0032] Specific implementation mode one: the method for obtaining clean long-term fish otoliths in this implementation mode is carried out according to the following steps:
[0033] 1. Pre-treat the fish, and then use dissecting scissors to cut off the head of the fish to obtain the fish head;
[0034] The fish is cryopreserved larvae, rancid larvae or dry larvae;
[0035] 2. Place the fish head under the dissecting microscope and adjust the magnification until the field of view is clear;
[0036] 3. Use a fine dissecting needle to insert into the skull obliquely forward from the two cheeks of the fish head, pull the two cheeks and the epidermis to the two sides respectively, until the occipital bone and ear bones are exposed, and the otoliths under the two cheeks can be seen;
[0037] 4. Press the upper cartilage of the otolith with a dissecting needle, squeeze out the lower otolith, take out the otolith with another dissecting needle, and remove the surrounding tissue and l...
specific Embodiment approach 2
[0047]Specific embodiment 2: The difference between this embodiment and specific embodiment 1 is that when the fish described in step 1 is the larvae preserved at low temperature, the specific operation is as follows: take out the larvae preserved at low temperature, thaw at normal temperature, Obtain the thawed larvae, and then cut off the head of the thawed larvae with dissecting scissors to obtain the fish head. Others are the same as the first embodiment.
specific Embodiment approach 3
[0048] Specific embodiment three: the difference between this embodiment and specific embodiment one or two is that: when the fish described in step 1 is rancid juvenile fish, the specific operation is as follows: dissect the rancid juvenile fish with dissecting scissors Cut off the head to get the fish head. Others are the same as those in Embodiment 1 or 2.
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Abstract
A method for acquiring a clean long-placed fish otolith relates to a method for acquiring the fish otolith. The invention aims at solving the problems in the prior art that the otolith acquired from long-placed fish more or less carries part of tissue and mucus, the otolith is in a state of a cluster of fluff after being rapidly dried, great interference is caused to subsequent detection, and the otolith acquired from rotting fish even carries a large quantity of dirt which is difficult to remove. The method comprises the following steps: preprocessing the fish, cutting off the head, getting out the otolith under an anatomical lens, removing surrounding tissue and lymph, dripping in a guanidine hydrochloride solution, washing with distilled water, and sealing up and fixing the otolith with a transparent nail polish or epoxy resin so as to obtain the clean long-placed fish otolith. According to the preparing method: the fish is pre-processed, and then, the otolith is taken out, soaked in the guanidine hydrochloride solution and then put in the distilled water to be soaked and stored so as to obtain the clean long-placed fish otolith. The method provided by the invention is used for acquiring the clean long-placed fish otolith.
Description
technical field [0001] The invention relates to a method for obtaining fish otoliths. Background technique [0002] Fish otoliths exist in the labyrinth of the eardrum in fish. There are three pairs in total. They are mainly composed of carbonate minerals and a small amount of organic matter and trace elements. Physiologically, they play the role of hearing and balance. Otoliths have attracted the attention of fish researchers very early, as an effective way of age identification, which is more accurate and easy to read than the annual rings on scales. Since Pannella first discovered the solar disc in 1971, there have been a lot of reports on various related studies using the solar disc, and otoliths have once become a research hotspot. [0003] As a living mineral, otolith has both the excellent characteristics of living organisms and minerals. It not only records the relevant information in the growth and life history of fish, but also marks the status and changes of its...
Claims
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Application Information
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Patent Type & Authority Applications(China)
IPC IPC(8): G01N1/34
Inventor 王继隆刘伟王臣
Owner HEILONGJIANG RIVER FISHERY RES INST CHINESE ACADEMY OF FISHERIES SCI
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