Method for distinguishing artificially-bred Gymnocypris przewalskii and naturally-bred Gymnocypris przewalskii and application

A technology of artificial breeding and naked carp, applied in the fields of application, climate change adaptation, fish farming, etc., can solve the problems of discomfort, high cost, and inability to distinguish between artificial breeding groups and natural breeding groups

Inactive Publication Date: 2016-06-22
INST OF AQUATIC LIFE ACAD SINICA +2
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Problems solved by technology

However, genetic markers cannot be used to distinguish artificial breeding populations from natural breeding populations because the parents of the artificially bred naked carp also come from natural spawning

Method used

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  • Method for distinguishing artificially-bred Gymnocypris przewalskii and naturally-bred Gymnocypris przewalskii and application
  • Method for distinguishing artificially-bred Gymnocypris przewalskii and naturally-bred Gymnocypris przewalskii and application

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Embodiment 1

[0025] (1) Sample collection: During the spring breeding season of naked carp in Qinghai Lake, five 1-year-old juveniles were collected from the four major rivers entering the lake (including Buha River, Heima River, Quanji River, and Shaliu River) that had not yet entered the lake. 5 pieces of 1-year-old juvenile fish cultured in the freshwater pond of Qinghai Lake Naked Carp Rescue Center; 5 naked carps of unknown birthplace captured in Qinghai Lake were used for the application analysis of the present invention.

[0026] (2) Separation and treatment of otoliths: Take out a pair of left and right micro otoliths of naked carp with carbon fiber tweezers, clean the soft tissue on the surface of otoliths with deionized distilled water, and then use Optima grade ultra-pure nitric acid (0.5% ) was cleaned under ultrasonic conditions, and then cleaned with ultrapure water under ultrasonic conditions, the cleaned otoliths were put into the prepared plastic molds, embedded with EPOFIX...

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Abstract

The invention discloses a method for distinguishing between artificial breeding and natural breeding of Qinghai Lake naked carp by adopting otolith element fingerprint marks, comprising the following steps: (1) collection of control samples; (2) separation and treatment of otoliths; (3) otoliths Elemental analysis: the ratios of Li:Ca, Mn:Ca, Mg:Ca, Sr:Ca and Ba:Ca of each sample were obtained as analysis parameters; (4) Discriminant analysis: the distribution discriminant analysis of SPSS 22.0 was used to divide the The discriminant analysis of the analysis parameters and the analysis parameters of the control samples can determine whether the Qinghai Lake naked carp whose birthplace is unknown is artificially bred or naturally bred. Using this marker for discriminant analysis, the artificially bred naked carp in Qinghai Lake can be successfully distinguished from the anadromous Qinghai Lake naked carp population, and the discrimination accuracy rate is 100%.

Description

technical field [0001] The invention belongs to the field of animal protection biology, and in particular relates to a method and application for distinguishing between artificial breeding and natural breeding of Qinghai Lake naked carps. Background technique [0002] Qinghai Lake is the largest inland saltwater lake in my country and a national wetland nature reserve. Grassland, birds and naked carps in Qinghai Lake wetland are the three major components of this nature reserve. Qinghai Lake naked carp (GymnocyprisprzewalskiiprzewalskiiQinghaiLakeNakedCraps), belonging to Gyprinidae, Schizothoracinae, and Gymnocypris, is the only economic fish in Qinghai Lake and is suitable for living in saline lakes. It is at the core of the entire ecological system of Qinghai Lake wetland, and the rise and fall of its resources will lead to the normal operation or failure of the entire ecosystem, or even collapse. The original resource reserve of naked carp in Qinghai Lake is about 320,...

Claims

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Application Information

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IPC IPC(8): A01K61/00
CPCY02A40/81A01K61/00
Inventor 章晋勇石琼朱志飞王敏马兴宇吴小江吴菲菲
Owner INST OF AQUATIC LIFE ACAD SINICA
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