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Qinghai lake naked carp embryo culture solution and preparation method thereof

A culture medium and embryo technology, applied in biochemical equipment and methods, embryo cells, culture process, etc., to achieve the effect of improving survival rate and low cost

Active Publication Date: 2021-02-26
CHINA ACAD OF SCI NORTHWEST HIGHLAND BIOLOGY INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

There is no report on the results of the research and development of Qinghai Lake naked carp embryo culture medium

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  • Qinghai lake naked carp embryo culture solution and preparation method thereof

Examples

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Effect test

Embodiment 1

[0053]Example 1 Preparation of culture solution for embryo culture and hatching of Qinghai Lake naked carp

[0054] ingredient content The content of each material component of mother liquor Mother liquor one 10 mL NaCl 0.8 g, KCl 0.04 g Mother liquor two 10 mL CaCl 2 0.144 g

Mother liquor three 10 mL MgSO 4 ·7H2O 0.246 g

Mother liquor four 1 mL Na 2 HPO 4 0.0035 g, KH 2 PO 4 0.006 g

NaHCO 3

0.35 g / Methylene blue 0.5 mg / Gentamicin Sulfate 40000 U / Deionized water Constant volume to 1000 mL /

[0055]The mother liquor can be stored at 4 °C for one month. When using, mix the specified amount into working fluid before use.

[0056]Mother liquor one configuration method:

[0057] ingredient content NaCl 8.5 g KCl 0.40 g Deionized water Constant volume to 100 mL

[0058]After the solid is completely dissolved, sterilize at 121°C for 15 min and store at 4°C.

[0059]Mother liquor two configuration method:

[0060] ingredient content CaCl 2

0.72 g Deionized water Constant volume to 50 mL ...

Embodiment 2

[0066]Example 2 Embryo Culture of Qinghai Lake Naked Carp

[0067]Collect fresh river water from the original site (water taken from the middle section of the Shaliu River) and transport it to the laboratory for later use.

[0068]The preparation method of the comparative culture solution: (The preparation method of the comparative culture solution is the same as in Example 1, the difference lies in the composition and content of the solution)

[0069] Mother liquor Contrast medium one Contrast medium two Contrast medium three Contrast medium four Contrast medium five Mother liquor one —10 mL 10 mL 10 mL 10 mL Mother liquor two 10 mL —10 mL 10 mL 10 mL Mother liquor three 10 mL 10 mL —10 mL 10 mL Mother liquor four 1 mL 1 mL 1 mL —1 mL NaHCO 3

0.35 g0.35 g0.35 g0.35 g— Methylene blue 0.5 mg 0.5 mg 0.5 mg 0.5 mg — Gentamicin Sulfate 40000 U40000 U40000 U40000 U— Deionized water Constant volume to 1000mLConstant volume to 1000mLConstant volume to 1000mLConstant volume to 1000mLConstant...

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Abstract

The invention discloses a Qinghai lake naked carp embryo culture solution and a preparation method thereof, and belongs to the technical field of fishery breeding. The culture solution comprises the following components: NaCl, KCl, CaCl2, MgSO4, Na2HPO4, KH2PO4, NaHCO3, methylene blue and gentamicin. The culture solution simulates the composition of a water body for hatching and growing Qinghai lake naked carp embryos under natural conditions, and other electrolyte ions and antibacterial components are added to prepare the Qinghai lake naked carp embryo culture solution, so that the survival rate of the Qinghai lake naked carp embryos can be remarkably increased; and the Qinghai lake naked carp embryo culture solution is low in cost and can be applied to biological experiments and large-scale artificial breeding of Qinghai lake naked carps.

Description

Technical field[0001]The invention belongs to the technical field of fishery breeding, and specifically relates to a Qinghai Lake naked carp embryo culture solution and a preparation method thereof.Background technique[0002]Naked carp in Qinghai Lake belongs to the family Cyprinidae, Schizoma subfamily, and belongs to the genus Naked carp. It is commonly known as "Huangyu" in Qinghai Lake. Since the 1970s, natural and man-made factors such as the continuous decline of the water level in the Qinghai Lake area, the gradual reduction of the marsh area in the lake area, overfishing and overfishing have caused the natural resources of Qinghai Lake naked carp to decline sharply and become an endangered species.[0003]The decline of naked carp resources will seriously affect the fragile Qinghai Lake ecosystem and destroy the fish-based "fish-bird symbiosis" ecological balance in Qinghai Lake. The protection of Qinghai Lake naked carp is highly valued by society, scientific research institut...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/073A01K61/10
CPCC12N5/0604A01K61/10C12N2500/12C12N2500/16C12N2500/14Y02A40/81
Inventor 刘思嘉田菲田得红王贺崐元李晓环赵凯
Owner CHINA ACAD OF SCI NORTHWEST HIGHLAND BIOLOGY INST
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