Method for marking fish otolith by fluorescent substances

A fluorescent substance, fish otolith technology, applied in application, fish farming, climate change adaptation, etc., can solve problems such as unfavorable release operation, process impact of release and recapture, large fish damage, etc., to avoid high loss rate , Easy to operate, reduce the effect of manpower and material resources

Inactive Publication Date: 2013-03-13
CHINA THREE GORGES CORPORATION +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, most of the commonly used methods of marking fish are physical treatment methods, such as: fin clipping, external tags, implanted marking lines, etc. These treatment methods not only require a lot of manpower, but also do more dam

Method used

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  • Method for marking fish otolith by fluorescent substances
  • Method for marking fish otolith by fluorescent substances

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Marking of silver carp:

[0019] (1) Dissolving tetracycline hydrochloride in water to obtain a tetracycline hydrochloride solution with a concentration of 300ppm;

[0020] (2) Soak the marked silver carp in the solution for 24 hours, and fill it with oxygen;

[0021] (3) When the soaking mark is over, put the fish body in clean water for temporary breeding, and feed it normally every day. After 7 to 10 days, you can take out the otoliths, clean the otoliths with absolute ethanol, and remove impurities on the surface of the otoliths. You can also use xylene After clearing, the otoliths were observed under a fluorescent microscope. It can also be kept in clear water for a while, fed normally, and the otoliths can be taken out for observation at different times. figure 1 It is the marked picture of otoliths of silver carp after soaking for 6 months, A is the control picture, and B is the marked picture; all pictures were taken under the blue excitation light.

[0022] ...

Embodiment 2

[0024] The steps for marking bighead carp are as follows:

[0025] (1) Dissolving tetracycline hydrochloride in water to obtain a tetracycline hydrochloride solution with a concentration of 300ppm;

[0026] (2) Soak the marked bighead carp in the solution for 24 hours, and aerobic;

[0027] (3) When the soaking mark is over, put the fish body in clean water for temporary breeding, and feed it normally every day. After 7 to 10 days, you can take out the otoliths, clean the otoliths with absolute ethanol, and remove impurities on the surface of the otoliths. You can also use xylene After clearing, the otoliths were observed under a fluorescent microscope. It can also be kept in clear water for a while, fed normally, and the otoliths can be taken out for observation at different times. figure 2 It is the marked picture of otoliths of bighead carp after soaking for 6 months, A is the control picture, and B is the marked picture; all pictures were taken under the blue excitation...

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Abstract

The invention relates to a method for marking fish otolith by fluorescent substances. According to the method, tetracycline hydrochloride is dissolved in water bodies, and marked solution of tetracycline hydrochloride solution is obtained; and fishes to be marked are put into the solution for soaking, and the marking of the fish otolith is completed. The tetracycline hydrochloride is adopted as the marking solution for soaking the fishes, the marking operation is completed, and the strong stress response damage and the fish body mechanical injury caused by artificial operation in the existing marking method are overcome; the investment of labor and materials is reduced; and the problem of high external hanging marker loss rate is solved. The method has the advantages that the cost performance is high, the operation is convenient, the soaking concentration and time can also be regulated according to different fishes, specifications and numbers, and the method belongs to a marking method capable of being applied to large-scale economic fish release.

Description

technical field [0001] The invention relates to a method for marking fish, in particular to a method for marking fish otoliths with fluorescent substances. Background technique [0002] Under the premise of the rapid reduction of fish resources in rivers, large-scale fish breeding and release projects organized by humans are becoming more and more frequent. To test whether artificial release can alleviate the poverty of river fishery resources, the method of marking and re-catching is usually used. , it is necessary to mark the fish before release, so as to facilitate the identification of recapture. [0003] Usually, the released fish body is marked and tracked back to detect the release effect. At present, most of the commonly used methods of marking fish are physical treatment methods, such as: fin clipping, external tags, implanted marking lines, etc. These treatment methods not only require a lot of manpower, but also do more damage to fish bodies Large, it is not con...

Claims

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Application Information

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IPC IPC(8): A01K61/00
CPCY02A40/81
Inventor 李云杨洪斌陈永柏陈大庆宋咏张薪胡颖雄李欣悦伍莎周乔
Owner CHINA THREE GORGES CORPORATION
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