Method for dyeing intermuscular bones of adult megalobrama amblycephala and application of method

A technology of bream and intermuscular bone, applied in the field of intermuscular bone dyeing, to achieve the effect of simple operation process, easy method and high ornamental value

Inactive Publication Date: 2016-05-04
HUAZHONG AGRI UNIV
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Problems solved by technology

Through searching, there is no literature reporting the method of staining the intermuscular bone of fish adults

Method used

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  • Method for dyeing intermuscular bones of adult megalobrama amblycephala and application of method
  • Method for dyeing intermuscular bones of adult megalobrama amblycephala and application of method
  • Method for dyeing intermuscular bones of adult megalobrama amblycephala and application of method

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Embodiment 2

[0045] The application of a method for staining the intermuscular bone of rice field eel in the study of intermuscular bone morphology of rice field eel, the steps are as follows:

[0046] 1. Take specimen: Anesthetize the adult rice eel sample with ice water mixture or urethane (urethane) aqueous solution, weighing 100-150g;

[0047] 2. Fix the adult bream bream specimen with 95% absolute ethanol for 3 days (when the specimen is fixed, if the solution becomes turbid, replace with 95% absolute ethanol; when changing the solution, pay attention to clear the fish in the container Impurities shed from the body table of the class standard);

[0048] 3. Transfer the rice field eel specimen to 1% (volume ratio) hydrogen peroxide soaking the specimen for about 12 hours to remove the pigment on the surface of the rice field eel;

[0049] 4. Transfer the rice field eel specimen to distilled water to rehydrate for 12 hours (wash off the impurities from the surface of the rice field eel in the c...

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Abstract

The invention discloses a method for dyeing intermuscular bones of adult megalobrama amblycephala. The method comprises the following steps: step 1, fixing an adult megalobrama amblycephala sample with absolute ethyl alcohol; step 2, rehydrating the adult megalobrama amblycephala sample with the absolute ethyl alcohol and distilled water; step 3, immersing the adult megalobrama amblycephala sample with a TBST solution overnight; step 4, immersing the adult megalobrama amblycephala sample with the distilled water and washing to remove the residual TBST solution; step 5, making the adult megalobrama amblycephala sample transparent with a newly-prepared KOH solution; step 6, transferring the sample to the new KOH solution and dropwise adding an alizarin red dyeing solution to dye until the solution becomes deep purple; step 7, transferring the sample into the new KOH solution and washing off the dyeing solution of the adult megalobrama amblycephala sample; and step 8, transferring the adult megalobrama amblycephala sample to glycerin to be dehydrated and preserved. The method is feasible and an operation process is simple; complicated instruments are not needed; the adult megalobrama amblycephala sample with a transparent body and the intermuscular bones which are dyed into the deep purple color, is obtained; and adhering positions and shapes of the intermuscular bones of the adult megalobrama amblycephala can be clearly observed from the sample.

Description

Technical field [0001] The present invention belongs to a method for dyeing intermuscular bone, and more specifically relates to a method for dyeing intermuscular bone of adult bream bream, and also relates to a method for dyeing intermuscular bone of adult bream bream. Morphology and use in the study of intermuscular bones in other fish of the same type. It is suitable for staining to obtain transparent specimens of adult bream bream, to clearly observe the attachment position and shape of the intermuscular bone of bream bream. Background technique [0002] Megalobramaamblycephala (Megalobramaamblycephala) belongs to Cypriniformes, Cypriniformes, Cypriniidae, Culterinae, Megalobrama. It is one of the important herbivorous economic fishes unique to our country. Because of its wide feeding habit, low cost, fast growth, high survival rate, easy fishing, and it has the advantages of delicious taste, small head, high meat content, good body shape, and moderate specifications. There...

Claims

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Application Information

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IPC IPC(8): A01N1/02
CPCA01N1/02
Inventor 高泽霞聂春红万世明张伟卓关柠楠王卫民
Owner HUAZHONG AGRI UNIV
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