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Method for marking stichopus japonicus individual and detecting mark

A marking and individual technology, applied in climate change adaptation, fluorescence/phosphorescence, animal husbandry, etc., to achieve the effect of cost saving, easy access to price, and reduction of operation steps

Active Publication Date: 2010-07-28
INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] In view of the lack of effective methods for marking sea cucumbers in the prior art, in order to overcome the above-mentioned defects of various existing marking methods, the purpose of the present invention is to provide a mark that is easy to operate, easy to identify, has a long mark retention time, and avoids rejection reactions. Sea cucumber individual and method for detecting the marker

Method used

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  • Method for marking stichopus japonicus individual and detecting mark

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] 1. Marking steps:

[0027] (1) Take 15 heads of 10±3.46g healthy sea cucumbers and temporarily raise them in a running water tank, fast for 24 hours, and continuously inflate (air);

[0028] (2) Dissolve 40mg of alizarin complex indicator in 1000mL of filtered seawater and stir continuously to completely dissolve the alizarin complex indicator (use an ultrasonic cleaner to speed up the dissolution if necessary);

[0029] (3) Put the japonicus japonicus into the solution and soak for 16 hours, during which the air (air) is continuously inflated, and the feeding is stopped.

[0030] (4) After 16 hours, all the sea cucumbers were taken out from the alizarin complex indicator solution, and the 15 sea cucumbers were eating and moving normally.

[0031] One month later, none of the sea cucumbers died, and the survival rate was 100%.

[0032] 2. Detection steps:

[0033] (1) Scraping the body wall epidermis or connective tissue of sea cucumbers with the size of small grains...

Embodiment 2

[0038] The difference from Example 1 is:

[0039] 1. Marking steps

[0040] (1) Take 30 healthy sea cucumbers of 4.49±1.27g and temporarily raise them in a running water tank, fast for 36 hours, and continuously inflate (air);

[0041] (2) Dissolve 160mg of alizarin complex indicator in 1000mL of filtered seawater and stir continuously to completely dissolve the alizarin complex indicator (use an ultrasonic cleaner to speed up the dissolution if necessary);

[0042] (3) Put the japonicus japonicus into the solution and soak for 24 hours, during which the air (air) is continuously inflated, and the feeding is stopped.

[0043] (4) After 24 hours, all the sea cucumbers were taken out from the alizarin complex indicator solution, and the 30 sea cucumbers were eating and moving normally.

[0044] One month later, 2 sea cucumbers died, and the survival rate was 93.3%.

[0045] 2. Detection steps

[0046] (1) Scraping the body wall epidermis or connective tissue of sea cucumbers...

Embodiment 3

[0051] The difference from Example 1 is:

[0052] 1. Marking steps

[0053] (1) Take 20 heads of 15±7.26g healthy sea cucumbers and temporarily raise them in a running water tank, fast for 48 hours, and continuously inflate (air);

[0054] (2) Dissolve 80mg of alizarin complex indicator in 1000mL filtered seawater and stir constantly to completely dissolve the alizarin complex indicator (use an ultrasonic cleaner to speed up the dissolution if necessary);

[0055] (3) Put the japonicus japonicus into the solution and soak for 48 hours, during which the air (air) is continuously inflated, and the feeding is stopped.

[0056] (4) After 48 hours, all the sea cucumbers were taken out from the alizarin complex indicator solution, and the 20 sea cucumbers were eating and moving normally.

[0057] One month later, one sea cucumber died, and the survival rate was 95%.

[0058] 2. Detection steps

[0059] (1) Scraping the body wall epidermis or connective tissue of sea cucumbers with...

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Abstract

The invention relates to the technical field of aquaculture, in particular to a method for marking stichopus japonicus and detecting the mark. When the stichopus japonicus individual is marked, fasting is performed on the stichopus japonicus for a period of time, then the stichopus japonicus is soaked for 16-48h with alizarin complexometric indicator. When the mark is detected, NaClO water solution is first employed to process stichopus japonicus body wall epidermis or connective tissue marked by the alizarin complexometric indicator, then the stichopus japonicus body wall epidermis or connective tissue are placed under a fluorescence microscope and detected. The marking method is completely performed when the stichopus japonicus normally grows, and no injection or anesthesia is needed to be performed on the stichopus japonicus, the stichopus japonicus living body is not damaged, operation processes for making are reduced and vigor decline of the stichopus japonicus is avoided and mortality rate thereof is reduced. In addition, the employed dye is cheap and easily available, thus saving costs for marking.

Description

Technical field: [0001] The invention relates to the technical field of aquaculture, in particular to a method for marking sea cucumber individuals and detecting the mark. Background technique: [0002] Sea cucumber has high nutritional, health and medicinal value. Sea cucumber is one of the edible sea cucumbers with the best quality, the widest distribution and the largest yield, and the most promising. The ever-increasing market demand has led to overfishing of wild sea cucumbers, resulting in the reduction of sea cucumber populations and the depletion of resources. Carrying out the multiplication and release of sea cucumbers is not only beneficial to restore sea cucumber resources and increase production, but also can improve and restore the damaged benthic environment by utilizing the disturbance of sea cucumbers to sediments. Marking sea cucumber can scientifically and accurately evaluate the effect of multiplication and release, and gain a deeper understanding of its ...

Claims

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Application Information

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IPC IPC(8): A01K61/00G01N21/64
CPCY02A40/81
Inventor 赵鹏邱天龙杨红生王清刘鹰
Owner INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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