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Madder extract and preparation method and application thereof

A technology of extract and madder, applied in madder extract and its preparation and application fields, can solve problems such as no research report on pharmacological activity

Inactive Publication Date: 2010-01-06
HENAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Pharmacological studies have shown that alizarin has a significant antibacterial effect, but there is no research report on other pharmacological activities of this compound

Method used

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  • Madder extract and preparation method and application thereof
  • Madder extract and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] This example is the extraction method of madder extract-ethyl acetate part, which specifically includes the following steps: take dry madder, crush, extract with 95% alcohol by percolation at room temperature, recover the ethanol to obtain the extract; dissolve the extract In water, heat to completely dissolve; then extract with ethyl acetate and concentrate to obtain ethyl acetate extract.

Embodiment 2

[0024] This example is a separation method of the compound alizarin, the separated product of the ethyl acetate extract of Rubia sinensis, which includes the following steps: the ethyl acetate extract is subjected to 200-300 mesh silica gel column chromatography, and petroleum ether-acetone gradient elution (95 :5~9:1), 4 parts are obtained. The third part is eluted by silica gel H column chromatography, petroleum ether-acetone (15:1), detected by TLC, and combined by Sephadex LH-20 column chromatography, washed with acetone After removal, the compound alizarin is obtained.

Embodiment 3

[0026] This example is an experiment on the treatment of diabetes with the ethyl acetate extract of rubia grass and the compound alizarin.

[0027] Method: Mouse Model of Alloxan Diabetes

[0028] Principle: Alloxan (Alloxan) is a pancreatic beta cell toxicant, which can selectively damage pancreatic beta cells in a variety of animals and cause diabetes. It is the most commonly used animal model of diabetes due to its simple and convenient method and high formation rate. For the stability study of the alloxan mouse diabetes model, the main method is to use the alloxan solution to induce diabetes after injection, and detect the blood glucose level in the mouse serum within a certain period of time. In diabetes, the level of GSP (glycated serum protein), which reflects the average blood glucose level in the past 1-2 weeks and the effect of drugs on non-enzymatic glycation in the body, will increase. At the same time, the activity of certain enzymes in the glycogen synthesis process ...

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Abstract

The invention relates to madder extract and a preparation method and an application thereof, wherein the madder extract is prepared from the following steps: pulverizing the rhizome of the madder, and using ethanol for percolation extraction at room temperature; recovering the ethanol and obtaining extract; dissolving the extract into water, and heating and completely dissolving the extract; and then, extracting the extract by using ethyl acetate, and concentrating the extract to obtain the madder ethyl acetate extract. The experiment indicates that the madder ethyl acetate extract and the compound alizarin can effectively reduce the blood glucose value, the GSP value, the TG value and the content of MDA in the tissue homogenate of the mouse with hyperglycemia caused by alloxan, and can obviously increase the SOD activity value of the hepatocyte of the mouse and increase the content of the hepatic glycogen of the tissue. The results prove that the madder ethyl acetate extract and the alizarin have good effect on reducing the content of the blood glucose.

Description

Technical field [0001] The invention relates to a preparation method of an ethyl acetate extract of Rubia madder and its application in the preparation of medicines for lowering blood sugar and treating diabetes; in particular, it relates to the application of alizarin, a compound isolated from this part, in preparation of medicines for lowering blood sugar and treating diabetes. Background technique [0002] Diabetes is a systemic disease characterized by hyperglycemia and metabolic disorders, and is closely related to insulin secretion. It is a clinical syndrome caused by the interaction of genetic and environmental factors. Absolute or relative insufficient insulin secretion and decreased sensitivity of target tissues to insulin cause a series of metabolic disorders such as sugar, protein, fat, water and electrolytes. Diabetes is mainly divided into two types: type I diabetes (ie insulin-dependent diabetes) and type II diabetes (ie non-insulin-dependent diabetes), the latter a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/74A61K31/122A61P3/10A61K125/00
Inventor 康文艺张丽宋艳丽
Owner HENAN UNIVERSITY
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