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Preparation method of MOFs (metal-organic frameworks)/PVA (polyvinyl alcohol) frozen gel immobilized laccase

A metal-organic framework, polyvinyl alcohol technology, applied in the direction of immobilization on/in organic carriers, biochemical equipment and methods, immobilization on or in inorganic carriers, etc., can solve the limitation of mass transfer capacity, time-consuming , small specific surface area, etc., to achieve the effect of multiple cycles, increase mass transfer capacity, and increase specific surface area

Active Publication Date: 2019-09-27
SHENYANG PHARMA UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the immobilized laccase prepared by the embedding method has a small specific surface area, and the mass transfer capacity is limited to a certain extent, so that the process of removing pollutants takes a long time.

Method used

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  • Preparation method of MOFs (metal-organic frameworks)/PVA (polyvinyl alcohol) frozen gel immobilized laccase
  • Preparation method of MOFs (metal-organic frameworks)/PVA (polyvinyl alcohol) frozen gel immobilized laccase
  • Preparation method of MOFs (metal-organic frameworks)/PVA (polyvinyl alcohol) frozen gel immobilized laccase

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Example 1 Preparation of metal-organic framework material / polyvinyl alcohol cryogel immobilized laccase

[0032] (1) Refer to literature "Yang, Q., Vaesen, S., Vishnuvarthan, M., Ragon, F., Serre, C., Vimont, A., Daturi, M., De Weireld, G., and Maurin, G. (2012). Journal of Materials Chemistry 22, 10210.", Synthesis of MIL-68(Al) by solvothermal method.

[0033] (2) Add quantitative polyvinyl alcohol (PVA) into the pure aqueous solution, heat and stir at 90° C. to completely dissolve the PVA, and obtain a PVA solution with a final concentration of 120 mg / mL.

[0034] (3) Add 8 mg of laccase (Lac) to 2 mL of water, mix well and add 2 mL of PVA solution and 240 mg of MIL-68 (Al) powder. After the mixture was vortexed, it was sonicated for 10 s to remove air bubbles.

[0035] (4) Use a pipette gun to take 200 μL of the mixed solution and drop it on a 1×2 cm glass slide, and spread the solution on the entire glass slide.

[0036] (5) Place the dripped glass slides in a -...

Embodiment 2

[0037] Example 2 Metal-organic framework material / polyvinyl alcohol cryogel performance detection method for immobilizing laccase

[0038] About the Characterization of Laccase Immobilized in MIL-68(Al) / PVA Cryogel

[0039] Observation of MIL-68(Al) surface, MIL-68(Al) / PVA cryogel and MIL-68(Al) / PVA cryogel-immobilized laccase surface and liquid nitrogen freezing brittle fracture using scanning electron microscope (SEM) The shape of the cross-section was obtained ( figure 1 ); the surface area and pore size distribution of MIL-68(Al) / PVA cryogel-immobilized laccase were characterized by nitrogen adsorption-desorption under 77K conditions, the results are as follows figure 2 Shown; MIL-68(Al) / PVA cryogel immobilized laccase and laccase powder in N 2 Under protection, the change of its thermal properties was measured by thermogravimetry and synchronous thermal analyzer at a heating rate of 10 °C / min ( image 3 ).

[0040] Enzyme activity determination of MIL-68(Al) / PVA cryo...

Embodiment 3

[0072] Referring to Examples 1 and 2, different metal-organic framework materials were prepared to prepare MOF / PVA cryogel-immobilized enzymes, and the relative enzyme activity, pH stability, and alizarin green removal rate were compared. The results are shown in Table 2. Show:

[0073] Table 2 Comparison of effects of immobilized laccase on various MOF-based cryogels

[0074]

[0075] The results showed that the relative enzyme activity of MIL-68(Al) / PVA cryogel-immobilized laccase (free enzyme activity was 100%) was 39.22%, which was the highest among the above-mentioned cryogel-immobilized laccases. Higher enzymatic activity recovery than most embedding methods (typically less than 20%). The ability of cryogel-immobilized laccase to remove organic pollutants is the key to evaluate the practical application of the material, among which PVA / Lac, MIL-68(Al) / PVA / Lac, MIL-100(Fe) / PVA / Lac and The removal rates of alizarin green by MIL-101(Cr) / PVA / Lac cryogel-immobilized lacc...

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Abstract

The invention belongs to the field of environmental protection and relates to a preparation method of MOFs (metal-organic frameworks) / PVA (polyvinyl alcohol) frozen gel immobilized laccase. The preparation method comprises the steps as follows: (1) synthesizing an MOF; (2) adding quantitative PVA to a pure water solution, and performing heating and stirring to enable PVA to dissolve completely to obtain a PVA solution; (3) adding laccase to water, adding the PVA solution and the MOF after uniform mixing, and removing bubbles by ultrasonic waves after uniformly mixing the mixture in a vortex manner; (4) dropping the mixed solution on a 1*2 cm slide, and applying the solution on the whole slide; (5) placing the slide in a refrigerator at subzero 20 DEG C to be frozen after dropping, taking out the composite, and repeating freezing-thawing cycles to obtain the MOFs / PVA frozen gel immobilized laccase finally. Dye alizarin green is used as a target pollutant, and the prepared MOFs / PVA frozen gel immobilized laccase has obviously increased removal rate for alizarin green in water.

Description

Technical field: [0001] The invention belongs to the field of environmental protection, and relates to a preparation method of a metal organic framework material / polyvinyl alcohol cryogel immobilized laccase. Background technique: [0002] Laccase (dioxygen oxidoreductase) belongs to the blue copper oxidase family, which was first discovered and isolated from lacquer by Japanese scholar Yoshida at the end of the nineteenth century. Laccases are widely distributed in nature and are found in prokaryotes, fungi, insects and higher plants, but their structures, properties and contents are quite different. Laccases can catalyze the degradation of various substrates, such as certain aromatic compounds (such as phenols, aromatic amines, thiophenols, etc.), dyes, pesticides, pharmaceuticals and personal care products (PPCPs), etc. Therefore, laccase is one of the research hotspots in the field of environmental protection. [0003] Laccase has good biotechnological potential, it ca...

Claims

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Application Information

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IPC IPC(8): C12N11/14C12N11/08C02F3/34C02F101/30
CPCC12N11/14C12N11/08C12N9/0061C02F3/342C12Y110/03002C02F2101/30Y02W10/37
Inventor 胡奇彭建侯晓虹
Owner SHENYANG PHARMA UNIVERSITY
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