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Young inkfish marking method, reagent and application

A juvenile squid, marking technology, applied to juvenile squid dyeing and marking method, aquatic animal marking, reagent and application field, can solve the problem of not suitable for needleless squid and other problems, and achieve the effect of good effect, low mortality rate and less waste.

Inactive Publication Date: 2014-07-02
MARINE FISHERIES RES INST OF ZHEJIANG
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But this method does not work for squid without needles

Method used

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  • Young inkfish marking method, reagent and application
  • Young inkfish marking method, reagent and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] The ALC reagent used was purchased from Dojin Chemical Research Institute of Japan Co., Ltd.

[0020] (1) Preparation of dyeing mother liquor:

[0021] Mix 100mg of alizarin complex indicator ALC powder with a small amount of distilled water, use a NaOH solution with a concentration of 0.1mol / L as a pH regulator, and set the volume to 50ml to prepare a dyeing mother liquor with a concentration of 2.0g / L for later use. The pH value is 8;

[0022] (2) Preparation of labeled injections: Dilute the above mother liquor with distilled water into a 350 mg / L injection, and the pH of the injection is 7-8.

[0023] Operation method:

[0024] 1) Anesthesia: Anesthetize the young squid to be dyed (such as Squid Mansoni) with a concentration of 5-10 mg / L eugenol solution or other anesthetic solution for 1-3 minutes; the selected young squid The trunk length is 20-40mm.

[0025] 2) Preparation before marking: operate on the tray, place a towel on the tray, and add water until the...

Embodiment 2

[0036] Use 0.1mol / L NaOH solution to adjust the pH to 9 to make a dyeing mother liquor with a concentration of 1.0g / L; then dilute the mother liquor with distilled water into a 300mg / L injection, and the pH of the injection is 8-9.

[0037] Anesthetize the juvenile squid to be marked (Needleless squid, carcass length 20-40mm) according to the method in Example 1, place it on the towel on the tray, inhale the above injection solution into a 1mL medical syringe, and insert the needle (needle diameter 0.33 mm) From the end of the young squid along the direction parallel to the endoskeleton, rotate and penetrate the endoskeleton for about 0.8cm, then pull out about 0.2cm, reserve a space and then inject, the injection volume is 0.6 ~ 0.8ml, until the color is complete Filled with endoskeleton.

[0038] Or, along the direction parallel to the endoskeleton, the needle rotates from the center of the side end (side) of the young squid and penetrates the endoskeleton for about 0.5cm, a...

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Abstract

The invention relates to the field of aquatic animals and in particular relates to an aquatic animal marking method and a marking injection agent. 3-aminomethylalizarin-N,N-acetic acid is used for preparing a young inkfish marking reagent, and a 3-aminomethylalizarin-N,N-acetic acid water solution with the content of 250-500ppm and the pH value of 6-10 serves as the injection agent to be injected to the endoskeleton of young inkfish. A dyeing marking method and the reagent have double effects of external marking and internal marking; the dyeing marking method is good in effect, convenient to operate and low in material waste; the death rate of the young inkfish is low; the marking color can be long kept and can be identified by naked eyes.

Description

technical field [0001] The invention relates to the field of aquatic animals, in particular to a method for marking aquatic animals, in particular to a dyeing and marking method for juvenile squid, reagents and applications. Background technique [0002] Mark release is one of the important means to study the distribution of aquatic organisms, estimate the natural population and evaluate the effect of artificial proliferation and release. The common marking methods at home and abroad include internal and external marking methods such as listing signs, fin cutting, fluorescent marking, metal wire code marking, separated satellite marking and biomarking. Practice shows that the most traditional listing is the most widely used and easier to recycle. sign law. Therefore, it is necessary to explore new marking methods, especially for the characteristic species of swimming organisms, in order to establish efficient marking methods, which can provide technical support for more acc...

Claims

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Application Information

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IPC IPC(8): C09B1/515A01K61/00
CPCY02A40/81
Inventor 梁君毕远新王伟定
Owner MARINE FISHERIES RES INST OF ZHEJIANG
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