35 results about "Hypermetabolism" patented technology

PROBLEM

There are provided induced malignant stem cells capable of in vitro proliferation that are useful in cancer research and drug discovery for cancer therapy, as well as processes for production thereof, cancer cells derived from these cells, and applications of these cells.

MEANS FOR SOLVING

An induced malignant stem cell capable of in vitro proliferation are characterized by satisfying the following two requirements:

• (1) having at least one aberration selected from among (a) an aberration of methylation (high or low degree of methylation) in a tumor suppressor gene or a cancer-related genetic region in endogenous genomic DNA, (b) a somatic mutation of a tumor suppressor gene or a somatic mutation of an endogenous cancer-related gene in endogenous genomic DNA, (c) abnormal expression (increased or reduced/lost expression) of an endogenous oncogene or an endogenous tumor suppressor gene, (d) abnormal expression (increased or reduced/lost expression) of a noncoding RNA such as an endogenous cancer-related microRNA, (e) abnormal expression of an endogenous cancer-related protein, (f) an aberration of endogenous cancer-related metabolism (hypermetabolism or hypometabolism), (g) an aberration of endogenous cancer-related sugar chain, (h) an aberration of copy number variations in endogenous genomic DNA, and (i) instability of microsatellites in endogenous genomic DNA in an induced malignant stem cell; and
• (2) expressing genes including POU5F1 gene, NANOG gene, SOX2 gene, and ZFP42 gene.

The invention discloses acid-sensitive controlled-release anti-inflammatory gel. The gel is formed by phenylboronic acid modified hyaluronic acid and tannic acid under the neutral to slightly alkalinecondition, and tannic acid can be released under the acidic condition. Amiloride and analogues or non-steroidal anti-inflammatory drugs thereof can be added to the anti-inflammatory gel. The invention also discloses a preparation method of the acid-sensitive controlled-release anti-inflammatory gel. The method comprises two steps including preparation of phenylboronic acid modified hyaluronic acid and preparation of the acid-sensitive controlled-release anti-inflammatory gel. The invention also discloses an application of the acid-sensitive controlled-release anti-inflammatory gel in preparation of drugs for treating physiological and pathologic change related diseases caused by decrease of pH value due to gathering of inflammatory metabolites. The gel has the lubricating function of hyaluronic acid, can relieve the stress of joints and promote backflow and update of metabolism, also has the non-specific anti-inflammatory, antioxidation, anti-effusion and analgesia functions of tannicacid, can inhibit excessive inflammatory response and body hypermetabolic reaction and is a new controlled-release anti-inflammatory gel.

The object of the present invention is to provide a method of preparing excellent cultured muscle cells having high metabolic capacity and insulin responsiveness, and further provide a method for the measurement of sensitive metabolic capacity using the cells. Moreover, its purpose is to provide a culture system/culture apparatus that can smoothly translocate such highly advanced cultured muscle cells intact to activity evaluation systems of a number of drugs. Moreover, the object of the present invention is to provide cultured muscle cells that are very suitable for measurement of the membrane-translocation activity of GLUT4 in an extraneous stimulus-dependent manner such as insulin, etc., and to provide a method for the measurement of the membrane-translocation activity of GLUT4 using the cells. The present invention is a method of preparing myotube cells, comprising a step (1) of culturing myoblast cells, a step (2) of differentiation-inducing the myotube cells into the myoblast cells in a culture medium with a high content of amino acids, and a step (3) of applying an electric pulse to the differentiation-induced myotube cells, and a method for the measurement of insulin-dependent sugar uptake using the myotube cells prepared by said method, and relates to the method for the measurement, comprising applying insulin stimulation by culturing the cells in a culture medium containing insulin, culturing the cells in the culture medium further supplemented with sugar, and measuring the sugar uptake. Furthermore, the present invention relates to a differentiation-type culture myotube cell constitutively expressing a recombinant GLUT4 having a labeled substance at its extra-cellular site, which is prepared by co-culturing wild-type myoblast cells and recombinant myoblast cells constitutively expressing said recombinant GLUT4, and a method for the measurement of membrane-translocation activity of the recombinant GLUT4 using the cells, and particularly a method for the measurement of insulin-dependent sugar uptake activity.

The invention provides a N(2)-L-alanyl-glutamine for injection and its preparing method, it has mixed N(2)-L-alanyl-glutamine and mannite to make N(2)-L-alanyl-glutamine whose mess percentage density is 50-100%. The invention has increased the stability of medicine greatly, convenient for transportation and storage, suitable for sicker in catabolism and hypermetabolism condition who needs glutamine.

The invention discloses special medical use formula nutrition powder for patients with lung diseases and a preparation method, and relates to the technical field of special medical use formula foods.The special medical use formula nutrition powder comprises the following components in parts by weight: 114.5-916 parts of protein substances, 107-856 parts of fat substances, 184-1472 parts of carbohydrate, 14.7-117.6 parts of composite minerals, 4.2-33.6 parts of composite vitamins, 0.4-3.2 parts of taurine, 0.5-4 parts of mono-and diglycerides of fatty acid, 0.09-0.7 part of nucleotide, 0.06-0.48 part of L-carnitine, 9-72 parts of a composite thickening agent, 1-8 parts of vanilla essence and 0.15-1.2 parts of aspartame, wherein the fat substances comprise 4.35-35 parts of perilla seed oilpowder, 15-120 parts of coconut oil powder, 25-200 parts of soybean oil powder, 15-120 parts of olive oil powder, 9-72 parts of linseed oil powder and 85-680 parts of medium chain triglyceride powder.Through the adoption of the high-energy, high-protein and low-carbohydrate formula, negative nitrogen balance caused by high metabolism and high consumption of the patients with the lung diseases canbe effectively improved, the generation of carbon dioxide is reduced, the burden of the lungs of the patients is avoided, and various vitamins and minerals are added, so that the immunity of the patients is improved.

This invention provides tools and methods that prevent a cancer cell from growing and reproducing more cancer cells. When growth ceases the body's immune defenses are enabled to attack and destroy these cells if the cancer cell itself has not initiated its own natural apoptotic self-destruction processes. The tools and methods of the invention obstruct the metabolic adaptions required to support cancer growth. By addressing the increased rates of metabolism characteristic of all rapidly reproducing cancer cells using chemical and/or physical nanotechnology to identify, segregate, isolate these hypermetabolizing cells, the body's immune system and other natural defenses are empowered to further isolate and eliminate the diseased cells. The extreme growth rates required for their rapid reproduction involve massively increased rates of the biochemical reactions supporting the cancerous growth. Each excess reaction produces extra heat and raises the internal cell's temperature and the tissue space immediate to the rapidly growing cells. This heat signature is used as a primary biomarker that enables binding of a nanoviral particle engineered to migrate to at attach at the target site at the site and prevent the cell from continued metabolism. Preferably, the nanoparticle not only binds and blocks external membrane receptors on the target cell, but incorporates into the rapidly metabolizing cells additional metabolic blocking agents to stop their growth. When cell growth and proliferation are stopped, the body's natural defenses are able to segregate and eliminate these cells. The massively increased rates of metabolic reactions characteristic of cancer cells also produce excess acid. The decreased pH is useful as a secondary or confirmatory marker for identifying these cancer cells.

The invention discloses a 2-trifluoromethyl-4-amino-quinoline derivative and application thereof. The 2-trifluoromethyl-4-amino-quinoline derivative comprises a compound as shown in the following general formula I. The compound prepared by the invention is used for preparing an anti-tumor active drug and has a good anti-tumor effect, the stability and the fat solubility of the compound are greatly improved, metabolic capability is not influenced, drug dosage is small, side effects are small, and high safety is achieved.

The invention discloses a health-care tea drink for metabolic syndromes and a preparation method of the health-care tea, and belongs to functional tea drinks. The health-care tea drink comprises, by mass, 15-25 parts of gynura medica, 15-25 parts of radix puerariae, 3-7 parts of ginseng, 8-15 parts of astragalus membranaceus, 7-13 parts of Chinese wolfberries, 7-13 parts of radix ophiopogonis, 5-11 parts of mulberry leaves, 3-7 parts of burdocks, 1-3 parts of pseudo-ginseng flowers and 5-11 parts of corn stigmas. The health-care tea drink is wide in application range, both patients suffering from diabetes, hypertension, hyperlipaemia and the metabolic syndromes and healthy people can drink the tea drink, the tea drink has good supportive therapeutic effect on people suffering from three highs and the metabolic syndromes, and curative effect of original drugs can be improved.

This invention provides tools and methods that prevent a cancer cell from growing and reproducing more cancer cells in a diseased animal. When growth ceases the body's immune defenses are enabled to attack and destroy these cells if the cancer cell itself has not initiated its own natural apoptotic self-destruction processes. The tools and methods of the invention obstruct the metabolic adaptions required to support cancer growth. By addressing the increased rates of metabolism characteristic of all rapidly reproducing cancer cells using chemical and/or physical nanotechnology to identify, segregate, isolate these hypermetabolizing cells, the body's immune system and other natural defenses are empowered to further isolate and eliminate the diseased cells. The extreme growth rates required for their rapid reproduction involve massively increased rates of the biochemical reactions supporting the cancerous growth. Each excess reaction produces extra heat and raises the internal cell's temperature and the tissue space immediate to the rapidly growing cells. This heat signature is used as a primary biomarker that enables binding of a nanoviral particle engineered to migrate to at attach at the target site at the site and prevent the cell from continued metabolism. Preferably, the nanoparticle not only binds and blocks external membrane receptors on the target cell, but incorporates into the rapidly metabolizing cells additional metabolic blocking agents to stop their growth. When cell growth and proliferation are stopped, the body's natural defenses are able to segregate and eliminate these cells. The massively increased rates of metabolic reactions characteristic of cancer cells also produce excess acid. The decreased pH is useful as a secondary or confirmatory marker for identifying these cancer cells.

The invention discloses a polyamino acid carrier with an acid-sensitive connecting arm in the middle as well as a preparation method and application of the polyamino acid carrier. The polyamino acid carrier contains a hydrophilic part, a hydrophobic part and a responsive connecting arm; a specific disulfide bond is introduced into the carrier design, the disulfide bond can be controllably broken into sulfydryl in vitro, the small molecule with the sulfydryl fragment or the gene with sulfydryl inherent on the surface is bonded with the protein drug again, and a covalent disulfide bond is formedto complete chemical bonding delivery of the drug. The polyamino acid carrier and the drug are bonded in a covalent bond manner, so that the drug can be stably transported in systemic circulation. When the carrier reaches high-metabolism and high-reducibility parts such as tumors or inflammations, disulfide bonds are broken again under the condition of high glutathione (GSH), and the activity ofthe drug is released and recovered. Therefore, the purposes of firstly masking the drug activity, then stably delivering the drug in vivo and recovering accurate regulation and control of the drug activity after the drug reaches a target position are achieved.

The invention discloses a dietary supplement, a preparation and an application of the dietary supplement and the preparation. The dietary supplement of the invention is prepared by the following raw materials comprising a konjac powder, mannan oligosaccharide, xylo-oligosaccharide, L-arabinose, and a mulberry leaf extract. Through increasing the sense of satiety, balancing and proliferating probiotics, and improving metabolism, the dietary supplement of the invention realizes that a dietary intake amount is not strictly controlled but the glycemic index is reduced, and the balance between intake and catabolism is gradually achieved, so that the dietary supplement is suitable for people who cannot relatively well control a dietary intake amount (patients with three high, metabolic syndrome,and obesity). After clinical verification, the curative effect is definite. After taking the dietary supplement for 1-5 days, the sense of satiety is clear, no abdominal fullness symptom appears, andpatients with hyperglycemia have different levels of blood sugar reduction from the next day. The effect of controlling the glycemic index and improving metabolism can be achieved without strictly controlling dietary intake.

The invention belongs to the technical field of extracting of fat-enriched proteins, and particularly relates to a fat-enriched protein in euphausia superba, and an application and a separating and detecting method of the fat-enriched protein. The fat-enriched protein is a protein-lipid compound, and the effects of reducing the content of in vitro lipid and improving metabolism level can be realized. The storage volume of the euphausia superba is great. The research confirms that the fat-enriched protein in the euphausia superba can limit and improve the situation of the blood fat of a rat suffering from hypercholesterolemia caused by high cholesterol diet, so that the fat-enriched protein in the euphausia superba can be applied to the development of health-care products having the efficacy of reducing blood lipid and reducing blood sugar. The invention provides an extracting method of the fat-enriched protein in the euphausia superba. Isoelectric precipitation cooperates with ultrasonic extraction, and then chromatographic separation is performed with a gel column, so that the fat-enriched protein in the euphausia superba is obtained. The extracting method is simple, convenient, and easy to operate; the obtained fat-enriched protein is high in purity and intensive; and when the fat-enriched protein is applied to the processing of health-care products and medicines, subsequentprocessing technologies can be saved.

The invention discloses a novel paeonol oxime compound as well as a preparation method and medical application thereof . A natural active ingredient paeonol with anti-platelet aggregation activity is used as a lead compound, 2-position phenolic hydroxyl and 1-position keto carbonyl of paeonol are used as modification sites, amino etherification is firstly performed on 2-position hydroxyl, improvement of metabolic stability and water solubility is facilitated, and then keto carbonyl oximation is performed on paeonol to improve the anti-platelet aggregation activity of paeonol. The invention particularly relates to a preparation method of a novel paeonol oxime compound and application of the novel paeonol oxime compound in pharmacy. The compound has an anti-platelet aggregation effect and can be used for preparing medicines for treating cardiovascular and cerebrovascular diseases.

The invention discloses a clean fine-core sterile heterogeneous sigmoid membrane as well as a preparation method and clinical application thereof. A clean fine-core sterile heterogeneous sigmoid membrane biological novel material is successfully manufactured. A pig sigmoid membrane is taken as an animal model and subjected to experimental investigation of substrate application. The heterogeneous sigmoid membrane prepared by the preparation method disclosed by the invention can block body stress response of a patient with extensive burns, can lower super-high metabolism and can keep the remaining skin structure necessary for later-period skin regeneration. After the wound surface is cleaned and sterilized in a treatment process, the dressings can be not changed, skin can be not grafted, operation can be not carried out, the wound surface is protected from pollution and complication is avoided, so that cure rate for extensive burns is improved; moreover, scar hyperplasia is restrained, and disability rate of the patient is reduced.

Thyrotoxicosis is called as hyperthyroidism, and is a disease with the symptoms of palpitation, sweating, eating and urination time increase and weight loss caused by body hypermetabolism and sympathetic nervous excitement as too many thyroid hormones are synthesized and released from thyroid. Most patients have symptoms of exophthalmos, palpebral oedema, hypopsia and the like. The medicine is prepared from medicines including horsetail, atractylodes macrocephala, dandelion, duckweed, cordyceps sinensis, arabic cowry shells, honeysuckle, roots of kudzu vine, polygonum multiflorum, felwort, desmodium triquetrum, angelica sinensis, patrinia, artemisia anomala, prepared rehmannia roots, fructus viticis, chlorite schist, cimicifuga foetida, kelp, rhizoma anemarrhenae, pumex, reed rhizome, cuttle bone, cape jasmine, turtle shells, prunella vulgaris, seaweed, pipewort, concha haliotidis, moutan barks, oyster, houttuynia cordata, callorhinus ursinus and hawksbill. Due to the effects of the combined medicines, a synergistic effect can be achieved, and thus hyperthyroidism can be effectively treated.

This invention provides tools and methods that prevent a cancer cell from growing and reproducing more cancer cells. When growth ceases the body's immune defenses are enabled to attack and destroy these cells if the cancer cell itself has not initiated its own natural apoptotic self-destruction processes. The tools and methods of the invention obstruct the metabolic adaptions required to support cancer growth. By addressing the increased rates of metabolism characteristic of all rapidly reproducing cancer cells using chemical and/or physical nanotechnology to identify, segregate, isolate these hypermetabolizing cells, the body's immune system and other natural defenses are empowered to further isolate and eliminate the diseased cells. The extreme growth rates required for their rapid reproduction involve massively increased rates of the biochemical reactions supporting the cancerous growth. Each excess reaction produces extra heat and raises the internal cell's temperature and the tissue space immediate to the rapidly growing cells. This heat signature is used as a primary biomarker that enables binding of a nanoviral particle engineered to migrate to at attach at the target site at the site and prevent the cell from continued metabolism. Preferably, the nanoparticle not only binds and blocks external membrane receptors on the target cell, but incorporates into the rapidly metabolizing cells additional metabolic blocking agents to stop their growth. When cell growth and proliferation are stopped, the body's natural defenses are able to segregate and eliminate these cells. The massively increased rates of metabolic reactions characteristic of cancer cells also produce excess acid. The decreased pH is useful as a secondary or confirmatory marker for identifying these cancer cells.

The invention discloses a method for predicting the treatment sensitivity of a BCL2 inhibitor, namely, Venotog, in acute myelogenous leukemia, and belongs to the technical field of biological medicines. According to the method disclosed by the invention, a model is established according to sample amino acid metabolism, fatty acid metabolism and glycolysis level, acute myelogenous leukemia patients are divided into four classes, and through drug sensitivity database analysis, the three classes, namely middle metabolism, are sensitive to the vinetoram targeted therapy, and the four classes, namely high metabolism, are resistant to the vinetoram targeted therapy are determined. The method can be used for efficiently evaluating and identifying patients sensitive to or resistant to Vinetoram, and providing a medication scheme of combining Vinetoram with etoram for the patients according to metabolic characteristics of the four types of drug-resistant patients so as to improve the treatment effect; and a tool is provided for analyzing the treatment sensitivity of the acute myelogenous leukemia patient to the Vinetoram and giving individualized treatment.

The invention relates to a compound feed additive for aquatic product breeding as well as a preparation and use method of the additive. The invention aims to explore and prepare the compound feed additive for abalone breeding by utilizing the anti-oxidation, anti-tumor, and immunity-improving functions of ciona intestinalis polypeptides, and the metabolic capability and disease resistance improving functions of probiotics. The feed compound additive is characterized in that the feed compound additive is formed by mixing the following components in percentages by weight: 80-85% of a ciona intestinalis polypeptide extraction liquid, 12-16% of the probiotics and 3-4% of multivitamins. The feed compound additive for the abalones provided by the invention has no adverse effect on the growth ofthe abalones, feeding tests show that after the compound additive is added into the feed, the abalone growth can be effectively promoted, the abalone own immunity can be improved, and the problem of young slow-growth poor-quality and low-immunity abalones can be completely solved.