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Fat-enriched protein in euphausia superba, and application and separating and detecting method of fat-enriched protein

An Antarctic krill and detection method technology, applied in the field of detection of the fat-rich protein, can solve the problems of low accuracy, difficulty, cumbersome processing, etc., and achieve the effect of saving purification and enrichment process and good extraction rate

Inactive Publication Date: 2019-11-05
SHANDONG NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The separation methods for lipoproteins mainly include ultracentrifugation and chemical precipitation. Among them, the quantitative operation in the process of ultracentrifugation is cumbersome and difficult, and the required sample volume is large. The chemical precipitation method has the advantages of less sample consumption and no special Advanced equipment, low cost, easy operation, etc., but the accuracy is low. In addition, methods for separating lipoproteins include gel filtration, high-pressure liquid chromatography, etc.
For example, Li Yiping and others disclosed the method for the isolation and purification of lipoproteins from the overwintering pine needle gall midge larvae, and Guan Xuechen and others disclosed the results of the separation and identification of lipoproteins in the hemolymph of armyworm larvae and the analysis of their physical and chemical properties, but the method is still There are disadvantages such as cumbersome pre-processing

Method used

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  • Fat-enriched protein in euphausia superba, and application and separating and detecting method of fat-enriched protein
  • Fat-enriched protein in euphausia superba, and application and separating and detecting method of fat-enriched protein
  • Fat-enriched protein in euphausia superba, and application and separating and detecting method of fat-enriched protein

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0056] Example 1: Extraction, separation and detection method of fat-rich protein from Antarctic krill

[0057] 1. Extraction and separation of Antarctic krill fat-rich protein, the steps are as follows:

[0058] (1) Take frozen Antarctic krill, thaw it in a refrigerator at 4°C for 4 hours, absorb the water from the thawed Antarctic krill, and crush the tissue into shrimp paste;

[0059] (2) Mix Antarctic krill mud (W) and deionized water (V) at a ratio of 1:3-5, adjust the pH value of the extract to 12.5 with 10mol / L NaOH aqueous solution, and perform ultrasonic extraction. The conditions are: 4°C, 300W, 40kHz extraction for 30min. Ultrasonic extraction is used to fully dissolve the fat-rich protein in the extraction solution.

[0060] (3) Centrifuge the above extract in a refrigerated centrifuge at 4°C and 4000r / min for 15min, collect the supernatant containing lipoprotein, repeat the operation of step (2) for the precipitated part, a total of three times, and combine the ...

Embodiment 2

[0074] Example 2: Extraction, separation and detection method of fat-rich protein from Antarctic krill

[0075]Take frozen Antarctic krill, thaw in a refrigerator at 4°C for 4 hours, absorb the water from the thawed Antarctic krill, and crush the tissue to make shrimp paste; take 10g of shrimp paste, add 30mL of deionized Water, mix well, adjust the pH value to 12.5 with 10mol / L NaOH solution, and perform ultrasonic extraction. The conditions of ultrasonic extraction are: 4°C, 300W, 40kHz extraction for 30min, so that the lipoprotein is fully dissolved in the extraction solution.

[0076] Centrifuge the extract solution containing lipoprotein in a refrigerated centrifuge at 4°C and 4000r / min for 15min, collect the supernatant containing lipoprotein, repeat the operation steps of lipoprotein extraction for the precipitated part, a total of three times, and combine the above Serum.

[0077] Use 4mol / L HCl solution to adjust the pH values ​​of the above supernatants to 4.5, then...

Embodiment 3

[0085] Example 3: Extraction, separation and detection method of fat-rich protein from Antarctic krill

[0086] Take frozen Antarctic krill, thaw in a refrigerator at 4°C for 4 hours, absorb the water from the thawed Antarctic krill, and crush the tissue to make shrimp paste; take 10g of shrimp paste, add 40mL of deionized Water, mix well, adjust the pH value to 12.5 with 10mol / L NaOH solution, and perform ultrasonic extraction. The conditions of ultrasonic extraction are: 4°C, 250W, 30kHz extraction for 30min, so that the lipoprotein is fully dissolved in the extraction solution.

[0087] Centrifuge the extract solution containing lipoprotein in a refrigerated centrifuge at 4°C and 4000r / min for 15min, collect the supernatant containing lipoprotein, repeat the operation steps of lipoprotein extraction for the precipitated part, a total of three times, and combine the above Serum.

[0088] Use 4mol / L HCl solution to adjust the pH values ​​of the above supernatants to 4.5, the...

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Abstract

The invention belongs to the technical field of extracting of fat-enriched proteins, and particularly relates to a fat-enriched protein in euphausia superba, and an application and a separating and detecting method of the fat-enriched protein. The fat-enriched protein is a protein-lipid compound, and the effects of reducing the content of in vitro lipid and improving metabolism level can be realized. The storage volume of the euphausia superba is great. The research confirms that the fat-enriched protein in the euphausia superba can limit and improve the situation of the blood fat of a rat suffering from hypercholesterolemia caused by high cholesterol diet, so that the fat-enriched protein in the euphausia superba can be applied to the development of health-care products having the efficacy of reducing blood lipid and reducing blood sugar. The invention provides an extracting method of the fat-enriched protein in the euphausia superba. Isoelectric precipitation cooperates with ultrasonic extraction, and then chromatographic separation is performed with a gel column, so that the fat-enriched protein in the euphausia superba is obtained. The extracting method is simple, convenient, and easy to operate; the obtained fat-enriched protein is high in purity and intensive; and when the fat-enriched protein is applied to the processing of health-care products and medicines, subsequentprocessing technologies can be saved.

Description

technical field [0001] The disclosure belongs to the technical field of fat-rich protein extraction, and specifically relates to an extraction and separation method for fat-rich protein in Antarctic krill, the fat-rich protein obtained by the method, an application, and a detection method for the fat-rich protein. Background technique [0002] The information disclosed in this Background section is only intended to increase the understanding of the general background of the disclosure, and is not necessarily to be taken as an acknowledgment or any form of suggestion that the information constitutes prior art that is already known to those skilled in the art. [0003] Lipoprotein is a protein-lipid complex. In animals, lipoprotein participates in the regulation of many aspects such as lipid transportation, membrane protein formation, winter resistance, etc., and plays an important role in maintaining basic physiological functions. effect. Changes in the composition and conte...

Claims

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Application Information

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IPC IPC(8): C07K14/435C12N9/08C12N9/12C12N9/76C07K1/14C07K1/16C07K1/30C07K1/36A61K38/01A61P3/06A61P3/10A23L33/17
CPCA23L33/17A23V2002/00A61K38/012A61P3/06A61P3/10C07K1/145C07K1/16C07K1/30C07K1/36C07K14/43509C12N9/0065C12N9/1223C12N9/6427C12Y111/01015C12Y207/03003C12Y304/21004A23V2200/326A23V2200/328A23V2250/542
Inventor 亢若雯王红灵刘代成
Owner SHANDONG NORMAL UNIV
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