Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

A kind of preparation method of truffle metabolite

A technology of metabolites and truffle bacteria, which is applied in the field of preparation of truffle bacteria metabolites, can solve the problems of artificial cultivation, harsh growth environment, and scarce output, and achieve the effects of significant curative effect, short production cycle, and low cost

Active Publication Date: 2020-06-19
吉林省生物研究所
View PDF3 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Truffles have extremely strict requirements on the growing environment, and cannot be artificially cultivated. The output is scarce and very expensive

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A kind of preparation method of truffle metabolite
  • A kind of preparation method of truffle metabolite
  • A kind of preparation method of truffle metabolite

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0025] As shown in the figure, a method for preparing a truffle metabolite comprises the following steps, and the following steps are carried out in sequence,

[0026] Step 1. Incline cultivation

[0027] The truffle strains isolated from wild truffles were inoculated into the potato dextrose agar medium with a pH value of 6-6.5 at an inoculation amount of 10% for slant culture. The culture temperature was 22°C-28°C and the culture time was 8 days to 10 days;

[0028] Step 2, liquid submerged fermentation seed culture is shake flask culture

[0029] The truffle bacteria strains cultivated in the step 1 are inoculated into the shake flasks according to the inoculation amount of 1:2 according to the ratio of the slope to the shake flask, and the built-in liquid culture medium in the shake flask is used for liquid submerged fermentation seed cultivation, and the culture temperature is 22 ℃ ~ 28 ℃, culture time 5 days to 7 days;

[0030] Step 3. Seed tank cultivation

[0031] ...

Embodiment 1

[0059] Slant strain culture: use PDA comprehensive medium, pH6.3, culture temperature 24 °C. The bacterium germinates quickly, and obvious colonies are formed within 2.5 days after inoculation, and the colonies are full of tubes within 10 days. The mycelia are initially brown, reticular, and branched. Through microscopic examination, it was obviously observed that the hyphae were thick, with many branches, well-developed lock-like joints, dark methylene blue staining, a large number of growth points, vacuoles, and no bacterial contamination.

[0060] Liquid Submerged Fermentation Seed Culture:

[0061] The used liquid culture medium composition weight percent in the shake flask is to include peptone 0.5%, yeast extract 0.23%, sucrose 4%, Kh in 1000g water 2 PO 4 0.2%, MgSO 4 ·7H 2 O 0.2%, PH value 6.3, high pressure 1.2kg / ㎝ 2 Sterilize for 30 minutes, inoculate after cooling, the inoculum amount is 1:6 ratio of slope to shake flask, that is, one slope connects 6 500ml sh...

Embodiment 2

[0068] Pharmacological test: The pharmacological activity and effective dosage of the fermented metabolites of truffle bacteria on endocrine disorders, reproductive system and behavior of menopausal animals were tested, and the results are reported as follows:

[0069] 1. Effects of fermentation products on estrogen and uterine coefficient in ovariectomized mice.

[0070] Compared with the normal control group, the serum E2 (estradiol) level in the model group was significantly lower (P<0.01), and the uterine coefficient was lower, indicating that the model was established. Compared with the model group, the dose group of 25ML / kg can significantly increase the weight of uterus and increase the coefficient of uterus, and the dose group of 25.20ml / kg can significantly increase the level of E2. The results are shown in Table 1.

[0071] Table 1 Effect of samples on estrogen and uterine coefficient in ovariectomized mice

[0072]

[0073] Compared with the model group *P<0.05...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a method for preparing metabolites of truffle bacteria, which belongs to the field of fungal biotechnology, and comprises five steps of inclined plane culture, shake flask culture, seed tank culture, large tank culture, and concentrated extraction. Finally, the pharmacological experiment data proves that the cultivation method disclosed in the present invention has the characteristics of low input cost, short production cycle, high expansion productivity and stable metabolites. The pharmacological activity and effective dosage of the fermented metabolites of truffle bacteria on the endocrine disorders, reproductive system and behavior of menopausal animals have been tested to prove that the fermented products of truffle bacteria have pharmacological activity on menopausal syndrome, and the curative effect is remarkable.

Description

technical field [0001] The invention belongs to the field of fungal biotechnology, and in particular relates to a method for preparing metabolites of truffle bacteria. Background technique [0002] Truffle is a fruiting body that grows and matures in the underground soil, also known as truffle. Truffles belong to Ascomycota, Pezizomycetes, Pezizales, and Tuberaceae in classification. Truffle is one of the most precious edible fungi among macrofungi. Contains rich nutrients and a variety of amino acids and inorganic salts that are easily absorbed by the human body. Therefore, Europeans call truffles underground gold, black diamond, caviar, and foie gras, which are listed as "the world's three most precious delicacies". Truffles have extremely strict requirements on the growing environment, and cannot be artificially cultivated. The output is rare and very expensive. Therefore, the edible and medicinal value of truffle urgently needs a new technical solution to be develope...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12P1/02C12R1/645
CPCC12P1/02C12N1/145C12R2001/645
Inventor 张金亭张智超黄勇张松玲夏宏志赵丽茹李金花秦玲
Owner 吉林省生物研究所
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com