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The present application relates to a kind of demulsifiers and its preparation method and application.The preparation method includes the following steps: (1) glycerol and p-hydroxy isophthalic acid are mixed, esterification is carried out, and branched prepolymer is obtained;(2) branched prepolymer is mixed with L-arginine, and amide reaction is carried out, and branched body is obtained;(3) branched body is mixed with carboxyl-terminated agent, and amide reaction is carried out, and the demulsifier is obtained.The demulsifier prepared by the specific preparation method can realize the efficient demulsification of high-salt crude oil emulsion, has the advantages of low toxicity, high demulsification efficiency, wide pH range, biodegradable.