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Separation and purification method of N(2)-L-alanyl-L-glutamine

A technology of dipeptide and purification method, which is applied in the direction of peptides, etc., can solve the problems of high cost, low yield and purity, and achieve the effects of simplifying production methods, high product purity, and shortening the production cycle

Inactive Publication Date: 2014-11-26
XIAMEN STARMEM TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to provide a kind of improved separation and purification method of CG dipeptide based on the advanced separation method of continuous chromatographic separation device, so as to reduce production cost and simplify The purpose of production method, shortening production cycle and improving total yield

Method used

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  • Separation and purification method of N(2)-L-alanyl-L-glutamine
  • Separation and purification method of N(2)-L-alanyl-L-glutamine

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Embodiment 1

[0017] In the prepared CG dipeptidase reaction liquid, the CG dipeptide mass fraction was 43.5%, and the sodium chloride mass fraction was 9.7%; after being filtered through a 50nm ceramic membrane, it was filled with a sodium-type strong acid ion exchange resin at a diameter-to-height ratio of 1:15, and the column temperature was 25 ℃, feed amount 0.1bv, constant temperature chromatographic separation, elution with distilled water, control column temperature 25 ℃ elution flow rate 0.5bv.h, collect the eluent and residual liquid respectively, the purity of glucodipeptide in the eluent is 96.8%, the concentration 35.2%, by distillation under reduced pressure, and then crystallized to obtain the dipeptide product, the yield is greater than 98.9%; the purity of sodium chloride in the residual liquid is 99.1%, and the concentration is 5.2%.

Embodiment 2

[0019] In the prepared CG dipeptidase reaction solution, the CG dipeptide mass fraction was 47.4%, and the sodium chloride mass fraction was 10.5%. After filtering through a 20nm ceramic membrane, the sodium-type strongly acidic ion exchange resin was filled with a diameter-to-height ratio of 1:20. 25°C, feed amount 0.5bv, constant temperature chromatographic separation, elution with distilled water, control column temperature 25°C elution flow rate 0.4bv.h, collect the eluent and residual liquid respectively, the purity of glucodipeptide in the eluent is 97.8%, Concentration of 37.5%, by distillation under reduced pressure, and then crystallization to obtain the product of dipeptide, the yield is greater than 98.5%; the purity of sodium chloride in the residual liquid is 97.8%, and the concentration is 7.2%.

Embodiment 3

[0021] In the obtained CG dipeptidase reaction solution, the CG dipeptide mass fraction was 44.4%, and the sodium chloride mass fraction was 8.5%,; after being filtered through a 100 μm ceramic membrane, the sodium-type strong acid ion exchange resin was filled with a diameter-to-height ratio of 1:50, and the column temperature 25°C, feed amount 1bv, constant temperature chromatographic separation, elution with distilled water, control column temperature 25°C elution flow rate 0.5bv.h, collect eluent and residual liquid respectively, distill under reduced pressure, and then crystallize, that is To obtain the dipeptide product of glucoside, the yield is greater than 97.5%; the purity of sodium chloride in the residual liquid is 98.9%, and the concentration is 6.3%.

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Abstract

The invention discloses a separation and purification method of N(2)-L-alanyl-L-glutamine, which is characterized by comprising the following steps: 1. dezymotization by ceramic membrane filtration: filtering a N(2)-L-alanyl-L-glutamine enzyme reaction solution through a ceramic membrane filter plant while washing with water, and removing the residual zymoprotein in the reaction solution, thereby obtaining a ceramic membrane dialysate; 2. continuous chromatography desalting: sending the ceramic membrane dialysate into a continuous chromatography separation plant to perform continuous chromatography separation so as to separate the N(2)-L-alanyl-L-glutamine from inorganic salts, thereby obtaining a N(2)-L-alanyl-L-glutamine solution; and 3. evaporation and crystallization: carrying out vacuum distillation on the N(2)-L-alanyl-L-glutamine solution obtained by continuous chromatography, and crystallizing to obtain the N(2)-L-alanyl-L-glutamine product. The method lowers the production cost, simplifies the production technique, shortens the production cycle, enhances the total yield, and has the advantages of high product purity and the like.

Description

technical field [0001] The present invention relates to the field of production of dipeptide, in particular to a method for separating and purifying dipeptide. Background technique [0002] Glycine dipeptide, scientific name N-(2)-L-alanyl-L-glutamine, is a modified compound of L-glutamine, with a relative molecular mass of 217.22 and is easily soluble in water. L-glutamine is an amino acid that is very abundant in the body and is a participant in the metabolism of various tissues in the human body. Lack of L-glutamine in the human body can cause various diseases. The parenteral use of L-glutamine has been limited due to its low solubility and instability. CG dipeptide can be decomposed into L-glutamine and L-alanine in the body, which makes it possible to supplement L-glutamine through parenteral nutrition infusion. The amino acids released by the decomposition of CG dipeptide are stored in the corresponding parts of the body as nutrients and metabolized according to the ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K5/062C07K1/36C07K1/34C07K1/30C07K1/16
Inventor 李振峰谢炜炫孙洪贵陈洪景虞美辉
Owner XIAMEN STARMEM TECH
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