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Serum-free adipose tissue-derived mesenchymal stem cell culture medium

A stem cell, serum-free technology, applied in animal cells, vertebrate cells, bone/connective tissue cells, etc., can solve the problems of uncertain chemical composition of serum-free medium, high price, and difficulty in ensuring the consistency of the medium. Achieve the effect of avoiding exogenous pollution, low cost, and avoiding batch differences

Active Publication Date: 2013-08-21
冯文峰
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

However, the existing serum-free medium or the chemical composition is uncertain, or the price is expensive, or it is difficult to ensure the consistency between medium batches

Method used

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  • Serum-free adipose tissue-derived mesenchymal stem cell culture medium

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Embodiment 1

[0033] A serum-free culture medium for adipose-derived mesenchymal stem cells, consisting of a basal medium and additional components; wherein the basal medium is DMEM-LG, and the additional components and their contents are as follows:

[0034] Basic fibroblast growth factor: 15ng / mL;

[0035] Epidermal growth factor: 15ng / mL;

[0036] Penicillin: 100U / mL;

[0037] Streptomycin: 100 μg / mL;

[0038] Heparin: 100μg / mL;

[0039] L-Glutamine: 5mM;

[0040] 2-Mercaptoethanol: 100 μM;

[0041] Leukemia inhibitory factor: 1000U / mL;

[0042] Sodium pyruvate: 1 mM.

[0043] The effects of culturing adipose-derived mesenchymal stem cells using the serum-free adipose-derived mesenchymal stem cell medium of the present invention are as follows.

[0044] 1. Take out the cryopreservation tube of adipose-derived mesenchymal stem cells separated by conventional collagenase I digestion method and stored in liquid nitrogen, and immediately put it into a 37°C water bath, shake slightly, ...

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Abstract

The invention relates to a serum-free adipose tissue-derived mesenchymal stem cell culture medium, which consists of a basic culture medium and added ingredients, wherein the basic culture medium is DMEM-LG, and the added ingredients and the content of each added ingredient are shown as follows: 5 to 20ng / mL of alkaline fiberblast growth factors, 5 to 20ng / mL of epidermal growth factors, 100U / mL of penicillin, 100 micrograms / mL of streptomycin, 50 to 200 micrograms / mL of heparin, 2 to 8mM of L-glutamine, 100 to 300 microM of 2-mercaptoethanol, 500 to 2000U / mL of leukaemia inhibitory factors and 0.5 to 2mM of sodium pyruvate. The serum-free adipose tissue-derived mesenchymal stem cell culture medium does not contain the serum, so that the inter-batch difference and the influence of the serum component on the cell culture can be avoided; the exogenous pollution of the serum and the toxicity of the serum on the cells can be avoided; and the ingredients are clear, so that the research of the psychological regulation mechanism of the cells can be facilitated.

Description

technical field [0001] The invention relates to a medium for mesenchymal stem cells, in particular to a medium for adipose mesenchymal stem cells without animal serum. Background technique [0002] Mesenchymal stem cells (Mesenchymal Stem Cells, MSCs) are important members of the stem cell family, derived from the mesoderm and ectoderm in the early stages of development. Mesenchymal stem cells were originally discovered in the bone marrow, and have attracted increasing attention because of their multi-lineage differentiation potential, hematopoietic support and promotion of stem cell implantation, immune regulation and self-replication. Under specific induction conditions in vivo or in vitro, mesenchymal stem cells can differentiate into fat, bone, cartilage, muscle, tendon, ligament, nerve, liver, cardiac muscle, endothelial and other tissue cells. It has multi-directional differentiation potential and can be used as an ideal seed cell for the repair of tissue and organ da...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/0775
Inventor 冯文峰佘志勇方海庆陈建兴
Owner 冯文峰
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