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A kind of cryopreservation solution and cryopreservation method of dental pulp mesenchymal stem cells

A technology of mesenchymal stem cells and cryopreservation method, which is applied in the field of dental pulp mesenchymal stem cell cryopreservation solution, can solve the problems of inability to guarantee the vitality of stem cells, affect the clinical utilization of stem cells, and damage dental pulp stem cells, so as to avoid adverse effects and avoid apoptosis. death and avoid structural damage

Active Publication Date: 2021-07-27
北京泰盛生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The current cryopreservation solutions will inevitably cause some damage to dental pulp stem cells during the cryopreservation process of cells, cannot guarantee the vitality of stem cells, and the cell proliferation rate is low, which affects the clinical utilization of stem cells

Method used

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  • A kind of cryopreservation solution and cryopreservation method of dental pulp mesenchymal stem cells

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Experimental program
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Effect test

Embodiment 1

[0026] A dental pulp mesenchymal stem cell cryopreservation solution, consisting of component A and component B, component A is a serum-free medium DMEM / F12 medium, component B is composed of the following raw materials: titanate nanotubes, 5 - disodium adenosine phosphate, vitamin E, 1,8-cineole, glycerol, the concentration of each raw material in the cryopreservation solution is: titanate nanotubes 6.5 μg / mL, disodium adenosine 5-phosphate 7.8 μg / mL mL, vitamin E1.5μg / mL, 1,8-cineole 10.2mg / mL, glycerin 25mg / mL;

[0027] Among them, the diameter of the titanate nanotube is 4-10nm, the length is 100-500nm, and the specific surface area is 200-300m 2 / g.

[0028] A cryopreservation method for dental pulp mesenchymal stem cells, comprising the following steps:

[0029] (1) The P3 generation dental pulp mesenchymal stem cells were resuspended in the serum-free medium of component A, and the cell density after resuspension was 2×10 6 pieces / mL, pipette evenly, and cool down to...

Embodiment 2

[0032] A dental pulp mesenchymal stem cell cryopreservation solution, consisting of component A and component B, component A is a serum-free medium DMEM / F12 medium, component B is composed of the following raw materials: titanate nanotubes, 5 - disodium adenosine phosphate, vitamin E, 1,8-cineole, glycerol, the concentration of each raw material in the cryopreservation solution is: titanate nanotubes 8.5 μg / mL, disodium adenosine 5-phosphate 8.0 μg / mL mL, vitamin E3.8μg / mL, 1,8-cineole 12.9mg / mL, glycerin 28mg / mL;

[0033] Among them, the diameter of the titanate nanotube is 4-10nm, the length is 100-500nm, and the specific surface area is 200-300m 2 / g.

[0034] A cryopreservation method for dental pulp mesenchymal stem cells, comprising the following steps:

[0035] (1) The P4 dental pulp mesenchymal stem cells were resuspended in the serum-free medium of component A, and the cell density after resuspension was 3×10 6 pieces / mL, pipette evenly, and cool down to 6°C;

[0...

Embodiment 3

[0038] A dental pulp mesenchymal stem cell cryopreservation solution, consisting of component A and component B, component A is a serum-free medium DMEM / F12 medium, component B is composed of the following raw materials: titanate nanotubes, 5 - disodium adenosine phosphate, vitamin E, 1,8-cineole, glycerin, the concentration of each raw material in the freezing solution is: titanate nanotubes 9.2 μg / mL, disodium adenosine 5-phosphate 8.5 μg / mL mL, vitamin E4.5μg / mL, 1,8-cineole 14.5mg / mL, glycerin 30mg / mL;

[0039] Among them, the diameter of the titanate nanotube is 4-10nm, the length is 100-500nm, and the specific surface area is 200-300m 2 / g.

[0040] A cryopreservation method for dental pulp mesenchymal stem cells, comprising the following steps:

[0041] (1) The P5 dental pulp mesenchymal stem cells were resuspended in the serum-free medium of component A, and the cell density after resuspension was 4×10 6 pcs / mL, blow evenly, cool down to 4-6°C;

[0042] (2) Add com...

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Abstract

The invention discloses a cryopreservation solution for dental pulp mesenchymal stem cells, which comprises component A and component B, component A is a serum-free medium, and component B is composed of the following raw materials: titanate nanotubes, 5-phosphoric acid Adenosine Disodium, Vitamin E, 1,8‑Cineole, Glycerin. The cryopreservation solution of the present invention does not add animal serum, which avoids possible exogenous pollution caused by animal serum. The 1,8-cineole and glycerol in component B act synergistically to protect the water in the cells from crystallization when the cells are close to the freezing point and avoid structural damage in the cells. Added titanate nanotubes, disodium adenosine 5-phosphate, and vitamin E, on the one hand, can maintain a certain activity of cells during cryopreservation in liquid nitrogen and avoid cell apoptosis; Helps cells recover quickly and restore activity. The present invention also provides a cryopreservation method for dental pulp mesenchymal stem cells. The cryopreservation solution of the present invention does not require complex programmed cooling and is easy to operate.

Description

technical field [0001] The invention relates to the field of stem cells, in particular to a cryopreservation solution and a cryopreservation method for dental pulp mesenchymal stem cells. Background technique [0002] Dental pulp mesenchymal stem cells (DPSCs) are a kind of mesenchymal stem cells with high proliferative ability and multi-lineage differentiation potential. The medical field has made great progress and has broad application prospects. At the same time, DPSCs can be induced to differentiate into bone tissue, cartilage tissue, adipose tissue, nerve tissue, muscle tissue, cornea and other tissue cells under appropriate conditions, and can be induced into induced pluripotent stem cells with characteristics similar to embryonic stem cells. It can be used as seed cells in the clinical transformation research of various diseases in the future. At present, many countries have established dental pulp stem cell banks for clinical treatment or scientific research needs...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N1/02
CPCA01N1/0221A01N1/0226
Inventor 杨莹
Owner 北京泰盛生物科技有限公司
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