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Liquid culture device for hairy roots

A liquid culture, hairy root technology, applied in the field of plant culture technology reactor engineering, can solve the problems of unfavorable hairy root stretching and growth, mechanical damage of hairy roots, uneven diffusion of dissolved oxygen, etc. The effect of preventing sexual pollution, preventing impurities from remaining, and stabilizing growth

Inactive Publication Date: 2016-11-09
大连德聚盛生态科技有限公司
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  • Abstract
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  • Claims
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AI Technical Summary

Problems solved by technology

[0004] In order to solve the problem that the oxygen supply method of the hairy root culture device in the prior art can easily cause the hairy root to be mechanically damaged, which is not conducive to the stretching and growth of the hairy root, the diffusion of dissolved oxygen is uneven, and it is easy to cause the culture solution to leak. The problem of exogenous pollution caused by adding nutrient solution, the present invention intends to provide an incubator with uniform diffusion of dissolved oxygen, which is more conducive to the growth of hairy roots

Method used

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  • Liquid culture device for hairy roots

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Embodiment 1

[0019] Hairy root liquid cultivator of the present invention, as figure 1 As shown, the tank body 1 is included, the top of the tank body 1 is a shrinking tank mouth 11, the tank mouth 11 is provided with a sealing cover 12, and the center of the sealing cover 12 is provided with a sealer 13, and the sealer is one-way air-permeable and does not A microporous sterile filter membrane through bacteria; the two shoulder walls of the tank body 1 are respectively provided with an air inlet 14 and a fluid addition port 15, and a groove 16 is arranged at the bottom of the tank body, and the groove 16 is fixed inside The gas generating device 2 is connected to the air inlet 14 through the air inlet pipe 3 . The air intake pipe 3 is a hard pipe, and its outside is covered with a layer of rubber sleeve hose; the air inlet 14 is equipped with a through-board joint, and the air intake pipe 3 outside the tank body is also connected with a filter 5 . The groove 16 is arc-shaped and is arran...

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Abstract

A liquid culture device for hairy roots comprises a pot body. The upper portion of the pot body is provided with a shrunk pot opening, an opening sealing cover is arranged at the pot opening, and an opening sealer is arranged at the center of the opening sealing cover; the two shoulder walls of the pot body are provided with a gas inlet and a liquid supplementing port respectively, and the liquid supplementing port is communicated with the interior of the pot body through a feeding pipe; a groove is formed in the center of the bottom of the pot body, and a gas generation device is fixed in the groove and connected with the gas inlet through a ventilation pipe. The culture device is made of a transparent high-temperature-resistant material, and the growth state of the hairy roots in the pot body can be observed in real time; the gas generation device is arranged at the bottom of the pot body, the fixing groove is formed, and therefore top-blowing oxygen supply is achieved; gas is exhausted through a filter membrane arranged on the opening sealing cover, and compared with the traditional design that a vent hole is formed in the bottom of a culture device, liquid leakage caused by the fact that an opening is formed in the bottom can be prevented, and extraneous contamination can be prevented; all joints in the pot body are in an arc shape, no dead corner exists in the pot body, therefore, impurity residues in the culture device are prevented, and liquid eddy can be reduced, so that growth of the hairy roots is stabilized.

Description

technical field [0001] The invention relates to a plant tissue culture device, in particular to a culture device for cultivating hairy root growth, which belongs to the field of reactor engineering of plant culture technology. Background technique [0002] The use of hairy root culture to produce medicinal plant secondary metabolites has great production potential, and the development of a bioreactor suitable for hairy root culture is the basis and key to its industrialization. At the level of shake flask culture, domestic and foreign scholars have established more than 100 hairy root culture systems and obtained different active compounds, but these hairy roots are finally used for expanded cultivation or industrial production. This is mainly due to: (1) Hairy roots are very easy to coalesce into tight balls or clusters, resulting in a decrease in dissolved oxygen inside the culture, leading to accelerated aging or even death of internal hairy roots, which ultimately affect...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M3/00C12M1/04
CPCC12M23/38C12M23/20C12M29/04
Inventor 张宗申王发德何连芳
Owner 大连德聚盛生态科技有限公司
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