A method for rapid detection of microbial drug resistance and a dedicated microfluidic chip

Abstract

The invention discloses a method for quickly detecting microbial drug resistance and a special microfluidic chip. The microfluidic chip is composed of a polydimethylsiloxane layer, an agarose layer and a transparent board which are orderly overlapped; two through holes are formed in the polydimethylsiloxane layer; the agarose layer is composed of the agarose and the microorganism and supported by the transparent board. The in-situ cultivation, gradient exposure and real-time observation of the microorganism can be realized by the microfluidic chip; the structure and operation are simple, the microfluidic chip can be quickly prepared and checked and is conveniently used for the ordinary biology lab; the injection pump is not needed; the microorganism does not need the fluorescence and the common microorganism is used; the key part of the chip can be used repeatedly, the cost is low and the special microfluidic chip is suitable for daily operation. The growing status of the microorganism in the medicines different in concentration can be observed in real time and the inhibition characters to the microorganism from the medicines can be quickly obtained; the minimum inhibition concentration (MIC) value, the half inhibitory concentration IC50 and the whole inhibition curve are achieved.

Description

technical field [0001] The invention relates to a method for rapidly detecting microbial drug resistance and a special microfluidic chip, belonging to the field of microbial drug resistance detection. Background technique [0002] The response characteristics of microorganisms under external environmental pressures such as antibiotics are very important for ecological security assessment and have been paid attention to by people. Among the response characteristics, the most concerned is the drug resistance of microorganisms, so various rapid detection methods have been developed and applied to determine the half-inhibitory concentration (IC 50 ) or minimum inhibitory concentration (MIC). [0003] The traditional method adopts the method of shaking flask or plate culture, adding different concentrations of antibiotics in the medium, and evaluating the inhibitory properties by observing the optical density value or colony count. The advantages of this method are accurate qua...

AI Technical Summary

Problems solved by technology

The advantage is that it is simple and fast, and the workload is small. The disadvantage is that the test paper is expensive, the incubation time is long, only the MIC value can be measured, and it is not suitable for studying the mixed effect of antibiotics, etc.

However, the above methods also have shortcomings, mainly including: (1) microorganisms are generally labeled with fluorescence for easy detection, but bacteria are generally difficult to label with fluorescence in actual tests; (2) completing the test on a chip requires a precision-controlled syringe pump, However, it is difficult to meet the requirements in ordinary laboratories or mobile field operations; (3) generally only the MIC value can be obtained, but it is difficult to obtain the IC 50 value or the entire inhibition curve

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