Microorganism in-situ culture chip suitable for different temperatures and method thereof

Abstract

The invention discloses a microorganism in-situ culture chip suitable for different temperatures and a method thereof. The chip comprises a first fixing plate, a culture plate, a contact plate and a second fixing plate; a plurality of first through holes penetrating through a plate body in the thickness direction are formed in the first fixing plate, and a plurality of second through holes penetrating through a plate body in the thickness direction are formed in the culture plate; a contact chamber penetrating through the thickness direction of a plate body is formed in the contact plate; anda plurality of cylindrical culture chambers for containing a solid culture medium or a liquid culture medium are fixed on the upper plate surface of the second fixing plate. Through structural optimization of the culture plate, liquid and solid culture media are switched according to high-temperature, low-temperature and normal-temperature habitat requirements, so that the applicable temperature of the in-situ culture chip is expanded, in-situ culture of microorganisms at different temperatures is realized, the temperature limitation of a traditional in-situ culture chip is broken through, anda basis is provided for in-situ culture of functional microorganisms under high-temperature media such as aerobic composting and anaerobic fermentation.

Description

technical field [0001] The invention belongs to the field of in-situ culture of microorganisms, and in particular relates to a microbe in-situ culture chip suitable for different temperatures and a method thereof. Background technique [0002] Most microorganisms cannot grow on standard agar medium, so only about 1% of microorganisms in the natural environment can be cultivated by existing culture methods. There are usually three explanations for 1% of cultivable microorganisms: the first explanation means that only 1% of the cells in a community can be cultured; the second explanation means that only 1% of the taxa in a community can be cultured; the third Interpretation refers to the growth of 1% of cells in a colony on standard agar medium. Different theoretical explanations indicate that only a small number of microorganisms can be cultured due to the limitation of traditional culture methods. Therefore, it is necessary to optimize the culture method to reduce the limi...

AI Technical Summary

Problems solved by technology

[0004] The invention overcomes the defect that the traditional microbial culture chip cannot realize the in-situ culture of microorganisms at different temperatures. By changing the structure of the culture plate and the assembly method with the contact plate, the switch between liquid and solid medium can be realized, and the growth of microorganisms can be realized at different temperatures. In situ culture: by improving the microorganism immobilization method, avoiding the residual medium and microbial cells on the culture plate during the solidification of the agar medium, providing a microbe in situ culture chip and its method suitable for different temperatures

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