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Novel drug screening microfluidic chip and preparation method thereof

A microfluidic chip and drug technology, applied in chemical instruments and methods, laboratory containers, laboratory utensils, etc., can solve problems without substantial breakthroughs, achieve good application prospects, simple operation, and high efficiency Effects of Drug Screening

Inactive Publication Date: 2013-04-17
SUZHOU WENHAO MICROFLUIDIC TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, using a microfluidic chip to prepare microstructures and microchannels on its surface, relying on the laminar flow effect between multiple layers of liquid in the microchannel to drive the sample microfluidics, and to complete the implantation of various cells at the same time, currently in a variety of The application field of cell co-culture has not yet had a substantial breakthrough

Method used

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  • Novel drug screening microfluidic chip and preparation method thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] The microstructure and microchannel graphics of the two-layer chip of the centrifugal microfluidic chip were designed and drawn by computer-aided design software. The microstructure and microchannel of the two-layer disc-shaped polymethyl methacrylate (PMMA) chip were processed and prepared by the numerical control CNC system, and the chips of each layer were cleaned with tap water and distilled water, and the remaining fingerprints, oil stains and other stains on the chip surface were wiped with ethanol . On the double-sided adhesive film, the required microstructures and microchannels were processed with a cutting machine. The two-layer chip is carefully aligned, glued, and pressure-sealed to make a microfluidic chip for co-culture of two kinds of cells. Add cell culture solution containing normal cells from the left sample injection hole and right sample injection hole respectively, add cell culture solution from the middle sample injection hole, and control the liq...

Embodiment 2

[0027] The microstructure and microchannel graphics of the two-layer chip of the centrifugal microfluidic chip were designed and drawn by computer-aided design software. The microstructure and microchannel of the two-layer disc-shaped polymethyl methacrylate (PMMA) chip were processed and prepared by the numerical control CNC system, and the chips of each layer were cleaned with tap water and distilled water, and the remaining fingerprints, oil stains and other stains on the chip surface were wiped with ethanol . On the double-sided adhesive film, the required microstructures and microchannels were processed with a cutting machine. The two-layer chip is carefully aligned, glued, and pressure-sealed to make a multi-cell co-cultured microfluidic chip. Add cell culture fluid containing normal cells from the left sample injection hole and right sample injection hole, add cell culture fluid from the middle sample injection hole, and control the liquid difference height between the...

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Abstract

The invention relates to a novel drug screening microfluidic chip and a preparation method. A microstructure and a micro channel are arranged on the surface of the microfluidic chip. Two types of cells are fixed in respective cell culture areas respectively. When the cells fill the cultivation areas and drugs are added, the reaction of cells to drugs during the process of growth can be visually researched with a microscope, as a result, a new thought and a new research technique for large-scale drug screening are provided. The novel drug screening microfluidic chip and the preparation method are applicable to fields relevant to cell biology, genetics, drug screening and the like. The microfluidic chip is simple to operate, achieves in-situ cultivation of cells, reduces the dosage of agent and samples, simplifies cell implantation process, effectively achieves drug screening, has the characteristics of portability, economy, high speed and high efficiency, and provides a novel analyzing technology for large-scale drug screening.

Description

technical field [0001] The invention relates to a new type of microfluidic chip for drug screening and a preparation method thereof. The microfluidic chip has microstructures and microchannels on its surface, and two kinds of cells are respectively fixed in respective specific cell culture areas, and the cell culture is covered with the culture area. Finally, adding drugs, the response of cells to drugs during the growth process can be visually studied under the microscope, which provides new ideas and new research technologies for large-scale drug screening, and is mainly used in cell biology, genetics and drug screening and other related fields. Background technique [0002] In the late 1980s, in order to overcome the damage to experimenters caused by drug screening, researchers worked hard to establish a culture system that was more similar to the in vivo environment, making the in vitro environment as consistent as possible with the in vivo environment, so that cells cou...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01L3/00
Inventor 沙俊
Owner SUZHOU WENHAO MICROFLUIDIC TECH CO LTD
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