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Frozen stock solution of neural stem cells and application method of frozen stock solution

A technology of neural stem cells and cryopreservation solution, which is applied in the fields of neurobiology and cell biology, can solve the problems of low recovery rate and achieve high recovery rate, strong antioxidant activity, and the effect of protecting structure and function

Active Publication Date: 2015-04-29
CENT SOUTH UNIV +1
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  • Abstract
  • Description
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Problems solved by technology

[0004] The purpose of the present invention is to provide a neural stem cell cryopreservation solution and its use method, solve the problem of low recovery rate of neural stem cells after cryopreservation, and improve the recovery rate of neural stem cells

Method used

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  • Frozen stock solution of neural stem cells and application method of frozen stock solution
  • Frozen stock solution of neural stem cells and application method of frozen stock solution
  • Frozen stock solution of neural stem cells and application method of frozen stock solution

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Embodiment Construction

[0015] The following in conjunction with specific embodiments is intended to further illustrate the present invention, rather than limit the present invention.

[0016] 1) Preparation of neural stem cell cryopreservation solution of the present invention:

[0017] 10ml neural stem cell cryopreservation solution contains the following components:

[0018]

[0019] Prepare the mother solution first, store it at -20 degrees, and then add it to the final concentration each time it is prepared.

[0020] 2) Cryopreservation of neural stem cells

[0021] Take the well-grown cells after 7-10 days of culture, and change the medium once 24 hours before collecting the cells. First use a pipette to blow gently in the primary culture bottle to float the cell balls that have settled at the bottom of the bottle. Then transfer the neurosphere suspension formed by the primary culture to a centrifuge tube, centrifuge at 800rpm / min for 3min. Discard the supernatant, and use the neural ste...

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Abstract

The invention discloses a frozen stock solution of neural stem cells and an application method of the frozen stock solution. The frozen stock solution contains B-27 Supplement without retinoic acid, L-Glutamine, EGF, FGF and vitamin E in Neurobasal-A medium. The frozen stock solution is low in cost; the problem of low thawing rate after existing neural stem cells are frozen is critically solved; and the frozen thawing rate of the neural stem cells is improved.

Description

technical field [0001] The invention belongs to the technical fields of cell biology and neurobiology, and in particular relates to a neural stem cell cryopreservation solution and a use method thereof. Background technique [0002] Neural stem cells are a type of cell population with proliferation, self-renewal ability and multi-directional differentiation potential, which can differentiate into various types of cells in neural tissues such as neurons, astrocytes and oligodendrocytes. Neural stem cells can differentiate into different types of cells in the central nervous system in vitro, which provides a good model for studying the development of the central nervous system. In addition, transplanting neural stem cells can treat acute and chronic spinal cord injuries and various nervous system diseases such as multiple sclerosis Alzheimer's disease, Parkinson's disease, ischemic brain injury, etc. have broad clinical application prospects. Since the effect of cryopreservat...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N1/02
Inventor 李志远邓思浩曹文宇卢大华徐杨
Owner CENT SOUTH UNIV
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