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Long-term preservation liquid for corneal tissue and preparation method thereof

A long-term preservation and organization technology, which is applied in the preservation, application, and animal husbandry of human or animal bodies. It can solve the problems of complex composition, high price, and restrictions on the wide application of corneal lenses, and achieve the effect of simple operation.

Inactive Publication Date: 2019-01-18
镇江雷音再生医学科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The corneal preservation solution currently on the market is only suitable for penetrating corneal transplantation or endothelial transplantation, and is suitable for low-temperature medium-term preservation of the cornea. It mainly preserves the activity of endothelial cells, and its composition is complex and expensive, but it limits its wide application in corneal lens preservation. application

Method used

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  • Long-term preservation liquid for corneal tissue and preparation method thereof
  • Long-term preservation liquid for corneal tissue and preparation method thereof
  • Long-term preservation liquid for corneal tissue and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] A long-term corneal tissue preservation solution, comprising the following components and contents:

[0037]

[0038] The preparation method of the above-mentioned corneal tissue long-term preservation solution specifically comprises the following steps:

[0039] Step S10: Weighing: weighing each component according to the formula amount.

[0040] Step S20: preparation: including:

[0041] Step S201: add some water for injection into the container, control the water temperature to 59±1°C, then add sodium chondroitin sulfate, sodium hyaluronate, dextran 40, sodium pyruvate, sodium bicarbonate, and 4-hydroxyethylpiperazine ethylsulfonate respectively acid, add water for injection and stir for 40min to dissolve the above components;

[0042] Step S202: Control the temperature of the mixture in step S201 to 39±1°C, add vitamin C, then add part of water for injection and stir for 10 minutes;

[0043] Step S203: Control the temperature of the mixture in step S202 to 39±...

Embodiment 2

[0049] A long-term corneal tissue preservation solution, comprising the following components and contents:

[0050]

[0051]The preparation method of the above-mentioned corneal tissue long-term preservation solution is basically the same as that of Example 1, the difference is that in step S201, the water temperature is controlled at 50±1°C and stirred for 80 minutes; in step S202, the temperature is controlled at 35±1°C and stirred for 20 minutes; In S203, control the temperature at 35±1°C, and stir for 15 minutes; in step S204, control the temperature at 35±1°C, and stir for 20 minutes.

Embodiment 3

[0053] A long-term corneal tissue preservation solution, comprising the following components and contents:

[0054]

[0055]

[0056] The preparation method of the above-mentioned corneal tissue long-term storage solution is basically the same as that of Example 1, the difference is that in step S201, the water temperature is controlled at 40±1°C and stirred for 100 minutes; in step S202, the temperature is controlled at 35±1°C and stirred for 30 minutes; In S203, control the temperature at 35±1°C, and stir for 25 minutes; in step S204, control the temperature at 35±1°C, and stir for 25 minutes.

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Abstract

The invention discloses long-term preservation liquid for corneal tissue and a preparation method thereof. The long-term preservation liquid is prepared from the following components: 15 to 25g / L of sodium chondroitin sulfate, 0.5 to 4g / L of sodium hyaluronate, 0.5 to 3 g / L of dextran 40, 15 to 30 g / L of glycerin, 0.001 to 0.3 g / L of sodium pyruvate, 0.002 to 2 g / L of vitamin C, 0.05 to 0.7 g / L ofgentamicin sulfate, 1.4 to 2.2 g / L of sodium bicarbonate, 5.0 to 6.5 g / L of 4-hydroxyethylpiperazine ethanesulfonic acid, and 1 L of water for injection. A combination system of sodium chondroitin sulfate, sodium hyaluronate and dextran is adopted to obtain the preservation liquid which can be used for long-term preservation of corneal tissue, thereby being particularly advantageous for maintaining an original collagen fiber structure and transparency of a corneal lens for a long term; after the cornea tissue is preserved by the long-term preservation liquid, the cornea tissue can be used directly after rewarming, the operation is simple, and rehydration is not needed.

Description

technical field [0001] The invention relates to a long-term corneal tissue preservation solution and a preparation method thereof. Background technique [0002] Corneal disease is the second leading cause of blindness in my country. There are about 4 million corneal blind patients in China, and more than 150,000 new corneal blind patients are added every year. Corneal transplantation is still the main means to restore vision and preserve eyeballs. Scarcity is a drop in the bucket compared with the huge demand. [0003] Femtosecond Laser Small Incision Lenticule Extraction (SMILE) is one of the biggest developments in the field of corneal refractive surgery in recent years. It is used for the correction of myopia and astigmatism, and has extremely high safety, effectiveness, Stability and predictability. This part of the tissue removed in the corneal stroma is called "corneal lens". The sources of SMILE corneal tissue lenses are very rich. About one million SMILE operations...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N1/02
CPCA01N1/021
Inventor 苏萍童剑萍许伟吴佳宇肖丹
Owner 镇江雷音再生医学科技有限公司
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