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Chemiluminiscent substrate solution and procalcitonin detection kit utilizing same

A detection kit and chemiluminescence technology, which is applied in chemiluminescence/bioluminescence, analysis by making materials undergo chemical reactions, and measurement devices, which can solve the problems of inability to meet the needs of trace detection, low sensitivity, and low sensitivity , to achieve excellent performance, reduce luminous background, and high sensitivity

Active Publication Date: 2016-05-25
CUSABIO TECH LLC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, procalcitonin detection kits on the market use colloidal gold detection, latex turbidimetry and ELISA methods. These methods have low sensitivity and cannot meet the needs of trace detection; The chemiluminescence method mostly uses dual reagents and a luminescent substrate solution with low sensitivity, which has high background value and low sensitivity

Method used

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  • Chemiluminiscent substrate solution and procalcitonin detection kit utilizing same
  • Chemiluminiscent substrate solution and procalcitonin detection kit utilizing same
  • Chemiluminiscent substrate solution and procalcitonin detection kit utilizing same

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Effect test

Embodiment 1

[0017] Embodiment 1 A kind of chemiluminescence substrate liquid

[0018] Embodiment 1 provides a kind of preparation method of chemiluminescence substrate liquid, weighs according to following component weight:

[0019]

[0020] Dissolve in 0.1mol / L Tris-HCl buffer solution with pH 9.0, and dilute to 1L.

[0021] Chemiluminescence substrate solution of the present invention and import chemiluminescence substrate solution (SuperSignalWestFemto substrate solution with the highest sensitivity of Thermo Company) and several common chemiluminescence substrate solutions contrast: with 50ul1ng / mL horseradish peroxidase as sample, Using deionized water as a blank control, add 100uL of the chemiluminescence solution of the present invention, the chemiluminescence substrate solution of Thermo Company and the chemiluminescence substrate solution containing several common enhancers, respectively, and measure the chemiluminescence signal on a chemiluminescence detector. As can be seen...

Embodiment 2

[0025] Embodiment 2 A kind of procalcitonin detection kit

[0026] Embodiment 2 provides a procalcitonin detection kit, comprising: avidin-coupled nano-magnetic particles, biotinylated anti-procalcitonin monoclonal antibody, horseradish peroxidase-labeled anti-procalcitonin monoclonal antibody Antibody, procalcitonin standard substance, and the chemiluminescent substrate solution of Example 1.

[0027] (1) Preparation method

[0028] 1. Standard product of procalcitonin

[0029] Pure PCT is PCT recombinant antigen, manufactured by Wuhan Huamei Bioengineering Co., Ltd., with a purity of 99%, a concentration of 1 mg / ml, and stored in Tris buffer. The PCT recombinant antigen was serially diluted, and the concentrations were 0, 0.08, 0.4, 2, 10, 60 and 100 ng / ml.

[0030] 2. Horseradish peroxidase-labeled anti-procalcitonin monoclonal antibody

[0031] 1) Dissolve 5 mg of HRP in 1 ml of distilled water to prepare an HRP aqueous solution.

[0032] 2) Freshly prepared 0.2M NaIO...

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Abstract

The invention discloses a chemiluminescent substrate solution and a procalcitonin detection kit utilizing the same. The chemiluminescent substrate solution is prepared from 0.8-1.5 g / L of luminol or its derivatives, 0.05-0.6 g / L2 of amino-4-nitro phenol, 0.01-0.05 mg / L of 9,9-dihexyl-2,7-dibromofluorene, 3-6 g / L of polyvinyl alcohol, 6-20 g / L of polyvinylpyrrolidone, 3-4.5 g / L of polyethylene glycol 600, 100-300 million units / L of gentamycin sulfate, 0.1-1.0 g / L of carbamide peroxide and 0.1 mol / L of a Tris-HCl buffer solution with the pH of 9.0. The chemiluminescent substrate solution is a single solution, can not only achieve the HRP enzyme catalyzed chemiluminescence reaction and can also effectively improve the luminous intensity and decrease a luminous background to achieve high-sensitivity detection.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a chemiluminescent substrate solution and a procalcitonin detection kit using the same. Background technique [0002] Chemiluminescent immunoassay is a widely used immunoassay method. The common chemiluminescent substrate solution is composed of A liquid oxidant and B liquid luminescent agent. When using, A liquid and B liquid are mixed. The mixing steps are cumbersome, and the mixing is not easy and uniform. It is also inconvenient to use. Most of the chemiluminescent liquids currently on the market are prepared in this way, and the steps are often cumbersome. Moreover, due to the limited effect of the enhancer, the sensitivity is also low. CN200410005756.1 discloses a substrate solution using p-iodophenol as an enhancer, but when only p-iodophenol is used as an enhancer, the background luminescence value is high, the sensitivity is low, and the luminescence intensity is low. CN200...

Claims

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Application Information

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IPC IPC(8): G01N21/76G01N33/577
CPCG01N21/76G01N33/577
Inventor 华权高沈鹤霄段旭徐春雷
Owner CUSABIO TECH LLC
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