67 results about "Colistin sulphate" patented technology

A pharmaceutical composition comprising an antimicrobial agent and an enhancer of an antimicrobial agent, wherein the enhancer of an antimicrobial agent is an inhibitor of gene, that by inactivating the gene product potentiates the effectiveness of the antimicrobial agent. In some embodiments, the pharmaceutical composition further comprises a pharmaceutically acceptable carrier. In some embodiments, the antimicrobial agent is an antimicrobial peptide such as a polymyxin, for example but not limited to colistin. In some embodiments of the present invention provides methods to treat and / or prevent infection of a subject with a microorganism by administering a pharmaceutical composition comprising an antimicrobial agent and an enhancer of an antimicrobial agent. In some embodiments, the present invention provides methods to inhibit growth of a microorganism by administering a pharmaceutical composition comprising an antimicrobial agent and an enhancer of an antimicrobial agent.

This invention relates to a method of treating otitis externa using a topical combination medication, including one or more antifungal agent such as, for example fluconazole, voriconazole, itraconazole, clotrimazole, amphotericin B, caspofungin, micafungin, terbinafine, naftifine, butenafine, amorolfine, ravuconazole, posaconazole, flucytosine, econazole, enilaconazole, miconazole, oxiconazole, saperconazole, sulconazole, terconazole, tioconazole, nikkomycin Z, anidulafungin (LY303366), nystatin, pimaricin, griseofulvin, ciclopirox, haloprogin, tolnaftate, and undecylenate and one or more antibacterial agent such as neomycin sulfate, polymyxin B sulfate, colistin sulfate, gentamycin, tobramycin, chloramphenicol, Ciprofloxacin, Ofloxacin, a penicillin compound, a cephalosporin compound, a macrolide compound, a fluoroquinolone compound, streptomycin, or kanamycin.

There is described a composition comprising a therapeutically active imidazole, and derivatives thereof, and an agent active on a bacterial cell surface selected from the group consisting of one or more of colistin, nisin, D-cycloserine, fosfomycin, fosfomycin trometamol, fosfomycin disodium and polymixin B, and derivatives thereof.

The invention discloses an amoxicillin colistin sulphate granule and a preparation method thereof. The amoxicillin colistin sulphate granule comprises the following ingredients in parts by weight: 1-25 parts of amoxicillin, 0.5-50 parts of colistin sulphate and 50-90 parts of additives, as well as an adhesive accounting for 0.5-8 parts of the weight sum of the ingredients, and the amoxicillin colistin sulphate granule is prepared by such steps as sieving, dissolving, mixing, drying and pelletizing. The amoxicillin and colistin sulphate in the invention can be uniformly mixed, thereby improving the solubility of the product in water, reinforcing the efficacy, ensuring the treatment effect, facilitating the quick recovery of animals, guaranteeing easy storage and low by-effect and reinforcing the treatment effect by drug combination; the amoxicillin colistin sulphate granule can be not only used for treating mixed infection of pigs, but also being conveniently applied on clinic and facilitating the prevention and treatment of group animal diseases so as to alleviate animal stress reactions and reduce the labor intensity of manual drug use.

The present invention discloses a method of detecting colistin, and a special enzyme linked immunosorbent kit thereof. The enzyme linked immunosorbent kit comprises the colistin and specific antibodies of the colistin; and the specific antibodies are polyclonal antibodies or monoclonal antibodies of the colistin. The kit adopts the high specificity monoclonal antibodies of the colistin, which guarantees the reliability of detection results, and the experimental results show that the kit has the characteristics of high specificity, high sensitivity, high precision, high accuracy and the like; and the main reagents of the kit all adopt the form of working liquid, the operation is convenient, and the cost is low. The operation of the method which adopts the kit to detect the colistin is simple, the detection time is shortened, the operation have low requirements for the pretreatment of the sample, and the method can be used for simultaneously and rapidly detecting the samples in bulk. Therefore, the method which adopts the enzyme linked immunosorbent kit for detection can play a significant role in the detection of the colistin.

The invention discloses a granular feed for laying chicks, and relates to a chicken feed. The granular feed comprises corn, wheat middling, bean pulp, cottonseed meal, rapeseed meal, corn albumen powder, fish meal, calcium hydrogen phosphate, fine rock powder, table salt, choline chloride, ferrous sulfate monohydrate, blue copperas, manganese sulfate monohydrate, zinc sulfate monohydrate, 1 percent sodium selenite, 98 percent lysine, 98 percent methionine, multiple chicken vitamins, 10 percent colistin, salinomycin, 1 percent calcium iodate and kitasamycin. The granular feed is prepared at a low temperature, so that the nutritional value of the feed is not destroyed, the crushing fineness of the feed is improved, and the feed has good palatability, is easily digested and can effectively reduce the respiratory diseases of chicks; and after the chicks are raised for 42 days by using 1Kg of the granular feed, the chick flock individuals are uniform, and the average weight can reach 480g and totally reaches the weight standard of medium laying chicks.

The invention discloses a 4% of premix for a growing pig and daily ration prepared by using the premix. The premix consists of composite trace elements, complex vitamins, colistin, virginiamycin, bacillus subtilis subspecies, phytase, sweetener, choline chloride, mountain flour, calcium hydrophosphate, lysine, methionine, threonine, tryptophan, salt, ethoxyquin, rice husk powder and zeolite powder. The daily ration is prepared by the premix, corns, soya bean meal and bran, the using amount of the soya bean meal is reduced by 7% absolutely, the shortage of the protein raw material soya bean meal is effectively relieved, the pig husbandry cost is reduced, and remarkable economic benefit and social benefit are produce. In addition, 18.62% of fecal nitrogen emission is reduced, and the ecological benefit is very remarkable.

The invention discloses a compound gentamycin sulfate soluble powder and a preparation method thereof, aiming at providing the compound gentamycin sulfate soluble powder and the preparation method thereof, which is used for treating pig bacterial enteritis and has the advantages of fast effect, safe use, small side effect and drug resistance ratio reduction of pathogenic bacteria. The compound gentamycin sulfate soluble powder comprises the following components in weight percent, 3% to 15% of gentamycin sulfate, 5% to 15% of maquindox, 3% to 15% of colistin sulphate, 2% of vitamin K3, and thebalance water. The invention adopts three antimicrobial components of the gentamycin sulfate, the maquindox and the colistin sulphate; the gentamycin sulfate and the colistin sulphate are difficult toabsorb when being eaten, and can kill the pathogenic bacteria through contact so as not to strengthen burden to pig bodies caused by drug; the maquindox is easy to absorb when being eaten, and is widely distributed in the bodies so as to effectively kill bacteria in the blood. The gentamycin sulfate, the maquindox and the colistin sulphate are combined for use so as to effectively kill the pathogenic bacteria, and greatly reduce the drug resistance ratio of the pathogenic bacteria causing the pig bacterial enteritis; and the vitamin K3 has the functions of stopping and restraining blooding.

The invention discloses a method for extraction of colistin sulphate. The method comprises the following steps of 1, a fermentation broth preparation process comprising adjusting a pH value to 4.0 by sulfuric acid, 2, a ceramic membrane filtration process comprising filtering the fermentation broth by a ceramic membrane, collecting a filtrate, and adjusting a pH value of the filtrate to 4.5-5, 3, a continuous moving bed absorption-desorption process, wherein through the continuous moving bed absorption-desorption process, a discharged liquid is obtained, 4, a discharged liquid decoloration process, 5, a nanofiltration concentration process, wherein in the nanofiltration concentration process, nanofiltration membrane pore sizes are in a range of 2100-400Da molecular weight cut-off and 10-20 times concentration is realized, and 6 a spray drying process. The method has a total colistin sulphate yield of 86 to 91% and has good energy-saving and emission-reduction effects.

The invention discloses a preparation method of a colistin sulfate premix. According to the method, colistin is extracted from fermentation liquid by pretreatment of the fermentation liquid, concentration by pressure reduction, separation by precipitation and granulation by a fluid bed, thereby preparing the colistin sulfate and then preparing the colistin sulfate premix. The invention has the advantages of strong specificity, short process, small equipment investment, little waste water, little pollution and high yield of 80-90%.

The invention discloses a compound neomycin sulphate solution and a preparation method thereof. The invention aims to provide a compound neomycin sulphate solution which has obvious effect on treating gastrointestinal infection of birds, treats symptoms and a root and reduces the medicine application frequency and a preparation method thereof. 100 L of solution comprises 2-15 kg of neomycin sulphate, 3-15 kg of colistin sulphate, 0.01-0.05 kg of scopolamine methobromide, 10 kg of alcohol with concentration of 95 percent and the balance of purified water. The invention adopts the neomycin sulphate and the colistin sulphate as antibacterial components, has double effects, can effectively reduce the medicine resistant rate of bacteria and has effect on common Gram negative bacteria causing intestinal infection; and the scopolamine methobromide inhibits glandular secretion, dries intestinal contents and lightens a dehydration symptom so as to have a rapid therapeutic effect.

The invention discloses a nosiheptide-colistin sulfate premixed agent which comprises 0.5-4 portions of nosiheptide, 1-10 portions of colistin, and the carriers of calcium carbonate, zeolite powder, corn flour, etc. for the rest in every 100 weight portions. The preparation method is as following. The raw materials are added in a mixing machine by the sequence of the carriers, the colistin sulfate, the nosiheptide and the carriers, and mixed evenly to make into powdery nosiheptide-colistin sulfate premixed agent. Or the nosiheptide and the carriers can be mixed fully firstly, then the colistin sulfate is dissolved in the water and sprinkled evenly on the mixture of the nosiheptide and the carriers. After being granulated, dried and screened, the mixture is made into the granular-typed nosiheptide-colistin sulfate premixed agent, the granularity of which is 30-60 mesh. The nosiheptide-colistin sulfate premixed agent of the invention is added in feedstuffs. The content range of the nosiheptide in every kilogram of overhead price feedstuff is 0.5-20mg, and the content range of the colistin sulfate in every kilogram of overhead price feedstuff is 2-40mg.

Monoclonal antibodies against bacterial proteins that confer resistance to the antibiotic colistin and hybridomas that produce such monoclonal antibodies are described. The monoclonal antibodies have a strong affinity for MCR proteins and may be used in detection methods and kits to detect the presence of MCR proteins including variants thereof in a sample.

The invention discloses a preparation method of colistin sulphate. In the preparation method, the colistin sulphate is prepared through fermentation liquor pretreatment, reduced-pressured concentration, heating, precipitation separation and spray drying by extracting colistin from fermentation liquor, and the total yield of the colistin sulphate reaches 75-85 percent. The preparation method disclosed by the invention has the advantages of high specifity and yield, short process, small equipment investment and low cost; and the environmental pollution is reduced because solvents are not adopted in the whole process.

The present invention provides vaccine compositions comprising OmpA, or antigenic fragments thereof, and related methods of active immunization against A. baumannii infection. The invention also provides antibodies and antigen-binding parts thereof that specifically bind to OmpA, and related methods of passive immunization against A. baumannii infection. The compositions and methods of the invention are useful for preventing or treating A. baumannii infections, including those caused by strains resistant to carbapenems and all other antibiotics except colistin or tigecycline, also referred to as extreme drug resistant (XDR) A. baumannii infections, and those resistant to every FDA approved antibiotic, also referred to as pan-drug resistant (PDR) A. baumannii infections.

The invention belongs to the technical field of biology and discloses a method for rapidly determining antibiotic resistance of Escherichia coli in a biofilm. The method comprises steps as follows: abiofilm growing stage; an antibiotic pressure stage; a biofilm recovery stage. According to the method, an in-vitro model of Gram negative bacteria such as Escherichia coli biofilm is established by adopting a micro-inoculation needle as a carrier, and the method is used for measuring BIC (biofilm inhibitory concentration) and MBEC (minimal biofilm eradication concentration) of antibiotics such aspolymyxin for the Escherichia coli biofilm. After the biofilm is formed for microorganisms, the degree of resistance to colistin is increased, BIC and MBEC values are both increased, and the MBEC value is larger than the BIC value. Compared with other methods, the in-vitro forming experiment of the Escherichia coli biofilm in the research has the characteristics of being simple to operate, easy to repeat and the like; meanwhile, few support devices are required, technical requirement is low, and errors caused by human factors and the like can be reduced; the method is applicable to larger samples; theoretical basis is provided for clinical rational drug use.

The invention discloses compound colistin sulfate soluble powder for treating lamb dysentery and a preparation method thereof and aims to provide the compound colistin sulfate soluble powder which hasa quick therapeutic effect on lamb dysentery, has efficacy in secondary infection bacteria and is convenient to use, and the preparation method which has a simple process and is easy to implement. The powder comprises the following components in percentage by weight: 2 to 10 percent of colistin sulfate, 5 to 20 percent of sulfathiazole sodium, 0.05 to 0.5 percent of atropine sulfate, 0.2 percentof vitamin K, and the balance of anhydrous dextrose. The soluble powder is a compound preparation of the combination of four medicaments, wherein the colistin sulfate and the sulfathiazole sodium areantibacterial for killing pathogens, the atropine sulfate controls diarrhea, reduces water loss and accelerates the treatment speed, and the vitamin K prevents and treats intestinal tract bleeding; and the four medicaments are mixed together reasonably so that the powder has a quick response, treats both symptoms and root causes, and can reduce the losses of raisers. The compound colistin sulfatesoluble powder is soluble powder, is soluble in milk, is used for feeding lambs through the milk and is more convenient to use.

The invention disclosed a kind of complex ofloxacin injection as well as preparing method. The injection contains 5-20 shares of ofloxacin, 5-25 shares of sulphuric acid colistin, 1-20 shares of trimethoprim and 50-300 shares of propylene glycol. The preparing procedure includes the following steps: (1) weighing materials; (2) putting the sulphuric acid colistin into injection water, stirring until it's all soluble; (3) heating propylene glycol to 75-85DEG C, adding trimethoprim, stirring to make it soluble; (4) putting together the solution of step (2) and (3), clarifying the solution, regulating the ph value of the solution, adding water to 1000 shares to get the preparation. Ofloxacin has the antibacterial activity of the third generation quinolones which can inhibit the bacteria prokaryotic cell DNA gyrase and DNA replication. Sulphuric acid colistin has no residue and side effects in animal bodies. The combination of the two medicines can widen the antibacterial spectrum and increase the curative effect.

The invention discloses an anti-diarrhea fast-growing concentrated feed for nursery pigs. The feed comprises the following raw materials: expanded soybean, import fish meal, whey powder, lysine, methionine, 536 type rovimix, vitamin E, vitamin C, choline chloride, piglet composite enzyme, phytase, honey, an acidifier, earth frankincense, calcium hydrogen phosphate, stone powder, table salt, ferrous sulfate monohydrate, zinc sulphate monohydrate, copper sulfate pentahydrate, manganese sulfate monohydrate, an iodine premixing agent, a selenium premixing agent, zinc oxide, arsanilic acid, tylosin, and colistin. The prepared concentrated feed (with the addition proportion of 25%) can allows the diarrhea rate of the nursery pigs to be controlled below 2%, allows the weight increment to be increased by more than 10%, and allows the feed conversion rate to be increased more than 8%.

The invention belongs to the field of antibiotic medicaments and in particular discloses a method for improving the total component content of colistin sulphate. By adding an alcohol solvent or a ketone solvent with no more than six carbon atoms into aqueous solution of the colistin sulphate, the total component content of the colistin sulphate is obviously improved to reach the standard of EP6.0. Besides, a high-purity colistin sulphate sample also can be obtained by the method of the invention. The method has the advantages of low cost, convenient recovery of the solvent, simple operation, suitability for industrial amplification and capacity of efficiently improving the purity of the colistin sulphate.

PmxA synthetase involved in polymyxin E synthesis, comprising four adenylation sites, characterized in that the second adenylation site has at least 90% identity with the peptide sequence SEQ ID NO 1:VTEAEKADLLGRFNDTTTEFPRGKTLIQLFEEQVERIPDAAAITLNEQELTYRELNERVNRLARTLRSHGISKGRLVAILAERSIEMVVGMLAAHKAGAAYVPIDPEYPEERIRFLIEDSGGQVMLTQSRLRERLAGSDPVILLDDESFYHEDGTNLNTGIEATDLACVIYTSGTTGKPKGNPVSHRNIVRVVQNTNYIDITERDHVLQLSSYSFDGATFDIFGALTNGARLVLVPYETLLEIGRLADLIQRERISVMFITTAFFNILVDVNVDCLRDVRAILFGGERVSVGHVRKALAHIGPGRLNHVYGPTESTVYTTYLPVDFVDELAVTVPIGRPISNTTVYIVDSRNKLLPIGVAGELCVGGEGLVRGYNNRPELTAEKFVDNPFVPGERMYRTGDLAKWLPDGTIEYVGRTDDQVKIRGFRIELGEIEAQLQKVEGIRKTTVFARENASGEKQLCAYYEADCELPAAELKSVLSKELPAYMIPAYLIQLERLPLTTNGKVDRRSLPAPEESLQPGGG,the underlined amino acids DGFFLGVVYK being conserved and forming a binding pocket specific for a leucine, isoleucine or valine residue.

The invention discloses a colistin sulphate purification method. The method disclosed by the invention is implemented by using a semi-preparative HPLC (high performance liquid chromatography) separation and purification system and taking a reversed-phase C18 as packing and taking a salt solution and acetonitrile as moving phases. The preparation of colistin sulphate according to the method is simple in operation, less in experimental steps, low in cost and short in cycle, and the purity of prepared colistin sulphate can be more than or equal to 98.0%.

The invention relates to a production method of feed colistin sulfate microcapsules, which comprises the following processing steps: 1. preparing a mixed solution of colistin sulfate and capsule material; 2. carrying out spray drying; and 3. adding a proper amount of antitackiness agent and auxiliary material and mixing. The production method has the advantages that the colistin sulfate is wrappedby polymer substances, so that the colistin sulfate is isolated from the external environment, not only protecting the effective valence of colistin, but also protecting vitamins and alkaline trace elements in feed from reacting with strongly acidic colistin through the isolation of a polymer film when the colistin and the feed are mixed. The polymer film is gradually dissolved after entering intestinal tracts, and colistin molecules are also released step by step to exert drug efficacy, improving the bioavailability of the colistin sulfate, prolonging the acting time of drugs, reducing the administration times, lowering the administration cost of raisers, and improving the economic benefit.

The invention discloses a method for extraction of colistin sulphate. The method comprises the following steps of 1, a fermentation broth preparation process comprising adjusting a pH value to 4.0 by sulfuric acid, 2, a ceramic membrane filtration process comprising filtering the fermentation broth by a ceramic membrane, collecting a filtrate, and adjusting a pH value of the filtrate to 4.5-5, 3, a continuous moving bed absorption-desorption process, wherein through the continuous moving bed absorption-desorption process, a discharged liquid is obtained, 4, a discharged liquid decoloration process, 5, a nanofiltration concentration process, wherein in the nanofiltration concentration process, nanofiltration membrane pore sizes are in a range of 2100-400Da molecular weight cut-off and 10-20 times concentration is realized, and 6 a spray drying process. The method has a total colistin sulphate yield of 86 to 91% and has good energy-saving and emission-reduction effects.

The invention belongs to the field of biotechnology and relates to preparation of animal sperm low-temperature and freeze preservation diluents, in particular to a horse sperm low-temperature and freeze preservation liquid. The sperm low-temperature and freeze preservation diluent is prepared by combining sodium bicarbonate, hydroxyethylpiperazine ethane sulfonic acid, trisodium citrate dihydrate, potassium citrate monohydrate, sodium hydroxide, lactose, glucose, inositol, gentamicin, neomycin, apramycin, colistin, amphotericin, sodium pyrophosphate, sodium caseinate, whey protein, an antioxidant and deionized water. The horse sperm low-temperature and freeze preservation diluent can effectively increase the survival rate of sperms obtained after unfreezing of low-temperature and freezing of horse seminal fluid and the hybridization conception rate. Compared with a conventional method, the method is easy and stable to implement and especially remarkable in technical effect.

The invention relates to a composition and the preparation method for livestock, poultry and aquatic animals. The virus disease vaccine oral liquid comprises the following materials according to the weight account: loosestrife 12 to 25 parts, humifuse euphorbia 15 to 25 parts, tuber of dwarf lilyturf 10 to 20 parts, phyllanfhus 5 to 20 parts, bitter almond 10 to 25 parts, prunella vulgaris 15 to 25 parts, balloonflower 10 to 20 parts, snake gall 2 to 5 parts, ox gall 10 to 20 parts, aloe 40 to 60 parts, crystal sugar 25 to 40 parts, rimantadine 5 to 10 parts, ribavirin 10 to 15 parts, and colistin 10 to 20 parts. The composition has the advantages of ability to reinforce antibody immunity, to relieve mutual interference among vaccines, and to reinforce the immune effect of the vaccine, and convenient drinking water and administration, high absorptivity, wide distribution, no drug residue or poison or side effect, and wide safety range.

The invention discloses a compound poplar flower oral liquid for treating piglet yellow or white dysentery and a preparation thereof, and aims to provide the compound poplar flower oral liquid which has a quick response to piglet yellow or white dysentery treatment, is effective and efficient and can reduce the drug resistance generated by bacteria and treat both symptoms and root causes, and thepreparation method which has a simple process and is easy to implement. Each 100 liters of the oral liquid comprises the following components by mass: 1 to 10 kilograms of poplar flower extract, 1 to10 kilograms of colistin sulfate, 0.05 to 0.5 kilogram of atropine sulfate, 0.01 kilogram of EDTA-2Na, 0.6 kilogram of sodium benzoate, and the balance of water. The compound poplar flower oral liquidis a compound preparation adopting the combination of Chinese medicaments and western medicaments, wherein the poplar flower and the colistin sulfate have a dual antibacterial effect; the atropine sulfate inhibits glandular secretion of intestinal tracts to reduce the water content of intestinal contents and can effectively reduce the drug resistance probability of escherichia coli; and thus, thecompound poplar flower oral liquid has a quick response to the piglet yellow or white dysentery treatment, is effective and efficient, and can obviously reduce the piglet death rate and improve the cure rate.

The present invention relates to the use of one or more compounds selected from the following: caffeic acid, thymol, aspirin, benzydamine hydrochloride, diclofenac sodium, flurbiprofen, ibuprofen, indomethacin, trifluoperazine hydrochloride, chlorprothixene hydrochloride, triflupromazine hydrochloride, suloctidil, thioridazine hydrochloride, dichlorophen, saccharin and piroxicam, in combination with a polymyxin selected from colistin or polymyxin B or a pharmaceutically acceptable derivative thereof, for use in the treatment of a microbial infection, and in particular for killing clinically latent microorganisms associated with microbial infections. The invention also provides a combination comprising suloctidil or a pharmaceutically acceptable derivative or prodrug thereof, and a polymyxin selected from polymyxin E and polymyxin B or a pharmaceutically acceptable derivative thereof. This combination is particularly useful for the treatment and / or prevention of microbial infections.

The invention discloses a preparation method of colistin methane sodium sulfonate, comprising the following steps: (a) adjusting the pH of a colistin sulphate aqueous solution by ammonia water or a sodium hydroxide solution to 9-13, stirring, reacting for 1-3 hours, filtering in vacuum, and adding deionized water into an obtained filter cake to prepare a colistin suspension; (b) adding methylolsulfonic acid sodium salt into the suspension, and reacting for 1-24 hours at the temperature of 10-90DEG C; (c) separating and purifying the reaction solution generated in step (b) by mixed resins; and (d) drying the product obtained in step (c). With the method disclosed by the invention, formaldehyde harm is fundamentally eliminated, the content of residual myxomycete in the product is reduced, and the quality of the colistin methane sodium sulfonate is improved.

The invention provides a method for improving probability of selecting forward mutation strains of polymyxin and relates to the field of screening of differential bacteria of bacillus polymyxa. The method comprises the following steps: adding light calcium carbonate into a separation plate culture medium; after preparing a plate, carrying out low-temperature refrigeration; carrying out strain culture on the separation plate; transferring to a slant culture medium added with the light calcium carbonate for culturing according to an appearance of a single colony; inoculating a bacterial lawn cultured on the slant culture medium into a seed culture medium for culturing, so as to obtain the strains with the forward mutation probability being more than 50 percent. The invention adopts a screening method combining metabolic regulation on colistin producing strains and regulation of initial humidity of a solid culture medium, so that a direct and distinguishable screening method of high-yield bacterial colonies is realized; the probability of obtaining the forward mutation strains by screening colistin is improved and the screening efficiency is improved. The method is simple in process and easy to operate. Therefore, large-scale screening can be obtained and more high-yield strains are obtained, and furthermore, the production cost is reduced.