Method for extraction of colistin sulphate

A colistin sulfate and extraction method technology, applied in the field of colistin sulfate extraction, can solve the problems of long cycle, high cost, serious pollution, etc., and achieve the effect of avoiding product activity and shortening the production cycle

Active Publication Date: 2014-12-10
SANDA FILM SCI & TECH XIAMEN +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The above route of the prior art has disadvantages such as complex process, long cycle, serious pollution, high cost, etc., for this reason a new process is needed to extract colistin sulfate

Method used

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  • Method for extraction of colistin sulphate

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] (7) Ceramic membrane filtration:

[0026] The fermented liquid has a titer of 550,000 U / ml, adjusted the pH to 4.0 with 40% sulfuric acid, and then filtered through a ceramic membrane. The pore size of the ceramic membrane is 0.5 microns, the operating temperature is controlled at 50°C, the membrane inlet pressure is 3bar, and the membrane outlet pressure is 1.5bar.

[0027] During the experiment, water was added twice, and the potency of the dialysate was about 200,000 U / ml; the yield of the ceramic membrane was 98.9%.

[0028] (8) Continuous moving bed adsorption analysis, resin column connection method see figure 1 :

[0029] The ceramic membrane filtrate enters the continuous moving bed system for adsorption and analysis. The moving bed adopts 30-6 type equipment (30-6 means that the number of columns is 30, and the inner diameter of the valve port of the equipment is 6mm), the rotation period is 24H, and the resin is filled with 21L. The resin is D152 Weakly acid...

Embodiment 2

[0036] (1) Ceramic membrane filtration:

[0037] The fermented broth has a titer of 600,000 U / ml, adjusted the pH to 4.5 with 40% sulfuric acid, and then filtered through a ceramic membrane. The ceramic membrane has a pore size of 0.5 microns, the operating temperature is controlled at 45°C, the membrane inlet pressure is 3.3bar, and the membrane outlet pressure is 1.9bar. During the experiment, water was added twice, and the potency of the dialysate was about 250,000 U / ml; the yield of the ceramic membrane was 99.2%.

[0038] (2) Continuous moving bed adsorption analysis:

[0039] The ceramic membrane filtrate enters the continuous moving bed system for adsorption and analysis. The moving bed adopts 30-6 type equipment (30-6 means that the number of columns is 30, and the inner diameter of the valve port of the equipment is 6mm), the rotation period is 24H, and the resin is filled with 21L. The resin is D152 Weakly acidic cationic resin, the moving bed is divided into feedin...

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Abstract

The invention discloses a method for extraction of colistin sulphate. The method comprises the following steps of 1, a fermentation broth preparation process comprising adjusting a pH value to 4.0 by sulfuric acid, 2, a ceramic membrane filtration process comprising filtering the fermentation broth by a ceramic membrane, collecting a filtrate, and adjusting a pH value of the filtrate to 4.5-5, 3, a continuous moving bed absorption-desorption process, wherein through the continuous moving bed absorption-desorption process, a discharged liquid is obtained, 4, a discharged liquid decoloration process, 5, a nanofiltration concentration process, wherein in the nanofiltration concentration process, nanofiltration membrane pore sizes are in a range of 2100-400Da molecular weight cut-off and 10-20 times concentration is realized, and 6 a spray drying process. The method has a total colistin sulphate yield of 86 to 91% and has good energy-saving and emission-reduction effects.

Description

technical field [0001] The invention relates to the field of chemical industry, in particular to a method for extracting colistin sulfate. Background technique [0002] Colistin Sulphate (Colistin Sulphate, also known as Colistin Sulfate, Anti-Adrene Sulfate, Colistin, Polymyxin E, Anti-Adrene, etc.) molecular formula is C 52 h 98 N 16 o 13 2H 2 SO 4 , the molecular weight is 1352. The pure product is white or off-white powder, easily soluble in water. Heat-resistant, difficult to absorb in the digestive tract, rapid excretion, low toxicity, no side effects, not easy to produce drug-resistant strains, it is one of the safest growth-promoting antibiotics for livestock and poultry. Colistin sulfate is a basic polypeptide antibiotic, which is mainly used to prevent and control the infection of sensitive bacteria and promote the growth of livestock and poultry. Colistin sulfate can bind to the free phosphate of cell membrane lipoproteins, which reduces the surface tensio...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K7/62C07K1/36C07K1/34C07K1/18
CPCY02P20/10
Inventor 林丽华杜明华何阿云许云鹏叶亚洲林雄水方富林蓝伟光
Owner SANDA FILM SCI & TECH XIAMEN
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