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Preparation method of colistin sulphate

A technology of colistin sulfate and colistin, which is applied in the field of bioengineering, can solve the problems of environmental pollution, many equipments, and low specificity, and achieve the effects of reducing environmental pollution, less investment in equipment, and strong specificity

Active Publication Date: 2011-11-23
浙江拜克生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The ion exchange method is used, which has long separation steps, many equipments, low yield, and brings a large amount of waste water, seriously polluting the environment; adopts the foam separation method to separate colistin sulfate, and Chinese patent application number 200510122187.3 discloses a foam separation method The method for preparing colistin sulfate by method, in the foaming process, other peptide substances and proteins will foam with the product at the same time, and will be brought into the product, affecting the purity of the product, the specificity is not strong, and the foaming uses a solvent, Pollution to the environment and high cost

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] (1) Pretreatment of fermentation broth

[0028] Adjust the pH of the fermented liquid to 2.5 with sulfuric acid, add 1%wt perlite filter aid while stirring, and filter through the plate and frame until no filtrate drips out to obtain a primary filtrate; the plate and frame filtration yield is 95%;

[0029] (2) Concentration under reduced pressure

[0030] Concentrate the primary filtrate under reduced pressure at a temperature of 78°C and a vacuum of -0.095MPa, and concentrate until the liquid phase content is 32300ug / ml to obtain a concentrated solution; the concentration yield is 98%;

[0031] (3) heating

[0032] Heat the concentrated solution to 88°C while stirring, keep it warm for 10 minutes, and then cool to room temperature to obtain a cloudy solution;

[0033] (4) suction filtration

[0034] Take 0.7g of perlite per 100ml of turbid liquid, add perlite filter aid into pure water, perform suction filtration after stirring to form a filter cake layer, and then ...

Embodiment 2

[0040] (1) Pretreatment of fermentation broth

[0041] Adjust the pH of the fermentation broth to 3.0 with sulfuric acid, add 3.5%wt diatomite filter aid while stirring, and filter through the plate frame until no filtrate drips out to obtain a primary filtrate. Plate and frame filtration yield was 96.5%;

[0042] (2) Concentration under reduced pressure

[0043] Concentrate the primary filtrate under reduced pressure at a temperature of 80°C and a vacuum of -0.098MPa, and concentrate until the liquid phase content is 31040ug / ml to obtain a concentrated solution; the concentration yield is 99%;

[0044] (3) heating

[0045] Heat the concentrated solution to 92°C while stirring, keep it warm for 25 minutes, and then cool to room temperature to obtain a cloudy solution;

[0046] (4) suction filtration

[0047] Take 0.6g of diatomite per 100ml of turbid liquid, add the diatomite filter aid into pure water, and perform suction filtration after stirring to form a filter cake la...

Embodiment 3

[0053] (1) Pretreatment of fermentation broth

[0054] Adjust the pH of the fermented liquid to 2.8 with sulfuric acid, add 2.5% wt of diatomite filter aid while stirring, and filter through the plate and frame until no filtrate drips out to obtain a primary filtrate; the plate and frame filtration yield is 94%;

[0055](2) Concentration under reduced pressure

[0056] Concentrate the primary filtrate under reduced pressure at a temperature of 75°C and a vacuum of -0.096MPa until the liquid phase content is 30800ug / ml to obtain a concentrated solution; the concentration yield is 98.5%;

[0057] (3) heating

[0058] Heat the concentrated solution to 93°C while stirring, keep it warm for 25 minutes, and then cool to room temperature to obtain a cloudy solution;

[0059] (4) suction filtration

[0060] Take 0.5g of diatomite per 100ml of turbid liquid, add the diatomite filter aid into pure water, and perform suction filtration after stirring to form a filter cake layer, and t...

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PUM

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Abstract

The invention discloses a preparation method of colistin sulphate. In the preparation method, the colistin sulphate is prepared through fermentation liquor pretreatment, reduced-pressured concentration, heating, precipitation separation and spray drying by extracting colistin from fermentation liquor, and the total yield of the colistin sulphate reaches 75-85 percent. The preparation method disclosed by the invention has the advantages of high specifity and yield, short process, small equipment investment and low cost; and the environmental pollution is reduced because solvents are not adopted in the whole process.

Description

technical field [0001] The invention belongs to the field of bioengineering and relates to the preparation of polypeptide antibiotics, in particular to a preparation method of colistin sulfate. Background technique [0002] Colistin is a multidrug-resistant Gram-negative pathogen (such as Pseudomonas aeruginosa, Pseudomonas aeruginosa ) Polypeptide antibiotics with specific resistance. Colistin contains diaminobutyric acid and has free amino groups, so it is alkaline. It is often used as a salt in clinical practice. Currently, it is commercialized in two forms. Colistin sulfate (colistin sulphate, CS) is one of the forms. Colistin sulfate can be used in animals such as pigs, poultry, cattle, and sheep. Because of its strong antibacterial effect, low residue, and resistance to drugs, it has been approved as a feed additive or veterinary drug by many countries such as China, the United States, the European Union, and Japan. It can treat animal feeding diseases caused by a va...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K7/62C07K1/14
Inventor 孙振华余炜储消和熊莉赵祖亮
Owner 浙江拜克生物科技有限公司
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