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Method for extracting gentamycin sulfate from gentamicin fermentation broth

A technology of gentamicin sulfate and gentamicin, applied in chemical instruments and methods, preparation of sugar derivatives, sugar derivatives, etc., can solve the problems of long steps, cumbersome process, low yield, etc., and achieve low hardness , the effect of reducing costs and improving product yield

Inactive Publication Date: 2016-11-09
HEBEI SHENGXUE DACHENG PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The method has long steps, complex process and low yield

Method used

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  • Method for extracting gentamycin sulfate from gentamicin fermentation broth

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Embodiment 1

[0029] Get gentamicin fermentation liquid 50L, potency 1500U / ml, containing product 0.075 billion. Adjust the pH to 2.0 with sulfuric acid, and filter the acidified solution with a ceramic membrane. The filtrate was adjusted to pH 6.0 with aqueous sodium hydroxide solution to obtain a neutralized solution. Pump the neutralizing solution into the D152 resin column for adsorption at a flow rate of 0.1BV / h. After the adsorption is saturated, pre-wash with 0.01mol / L sodium hydroxide aqueous solution at a flow rate of 0.1BV / h. When the titer is given, the prewashing is considered to be over. After pre-washing, elute with 0.1mol / L sodium hydroxide aqueous solution at a flow rate of 0.1BV / h until the pH of the effluent reaches 11.0, and top-wash with water until the titer is less than 100u / ml. The analytical solution is decolorized with 711 resin, and the decolorized solution is concentrated and desalted with a nanofiltration membrane with a molecular weight of 200. Sulfuric acid...

Embodiment 2

[0031] Gentamicin fermented broth 55L, potency 1450U / ml, product 0.0798 billion. Adjust the pH to 4.0 with sulfuric acid, and filter the acidified solution with a ceramic membrane. The filtrate was adjusted to pH 8.0 with aqueous sodium hydroxide solution to obtain a neutralized solution. Pump the neutralizing solution into the D152 resin column for adsorption at a flow rate of 3.0BV / h. After adsorption saturation, pre-wash with 0.1mol / L sodium hydroxide aqueous solution at a flow rate of 1BV / h. When the titer is given, the prewashing is considered to be over. After pre-washing, use 1.0mol / L sodium hydroxide aqueous solution for elution, the flow rate is 1BV / h until the pH of the effluent reaches 12.0, and top wash with water until the titer is less than 100u / ml. The analytical solution is decolorized with 711 resin, and the decolorized solution is concentrated and desalted with a nanofiltration membrane with a molecular weight of 270. Add sulfuric acid to the concentrated...

Embodiment 3

[0033] Get gentamicin fermentation liquid 60L, potency 1550U / ml, product 0.093 billion. Adjust the pH to 3.0 with sulfuric acid, and filter the acidified solution with a ceramic membrane. The filtrate was adjusted to pH 7.0 with aqueous sodium hydroxide solution to obtain a neutralized solution. Pump the neutralizing solution into the D152 resin column for adsorption at a flow rate of 1.0BV / h. After adsorption saturation, pre-wash with 0.05mol / L sodium hydroxide aqueous solution at a flow rate of 0.5BV / h. When the titer is given, the prewashing is considered to be over. After prewashing, use 0.5mol / L sodium hydroxide aqueous solution for elution, the flow rate is 0.5BV / h until the pH of the effluent reaches 11.5, and top wash with water until the titer is less than 100u / ml. The analytical solution is decolorized with 711 resin, and the decolorized solution is concentrated and desalted with a nanofiltration membrane with a molecular weight of 200. Add sulfuric acid to the c...

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Abstract

The invention aims to provide an improved method for extracting gentamycin sulfate from gentamicin fermentation broth. The method comprises the steps of fermentation broth filtration, adsorption through weakly acidic cation exchange resin, resolving, concentration, salt conversion and spray drying and the like. Gentamicin is one of main antibacterial drugs used for treating various gram-negative bacteria infections clinically at present and is a multicomponent mixture composed of 2-deoxystreptamine, magenta glycosylamine and garosamine and the like. According to the extraction method, the adsorption is performed through the cation exchange resin, resolving is performed through sodium hydroxide solution, decoloration is performed through anion exchange resin, nanofiltration concentration desalting is performed, finally salt conversion is performed, active carbon decoloration is performed, and the finished product is obtained through spray drying. The method has the advantages of being high in production efficiency, less in environmental pollution and good in finished product quality.

Description

technical field [0001] The invention relates to a method for extracting and preparing raw materials, in particular to a method for extracting gentamicin sulfate from gentamicin fermentation liquid. technical background [0002] Gentamicin is currently one of the main antibacterial drugs clinically used for various Gram-negative bacterial infections. It is a multi-component mixture composed of 2-deoxystreptamine, crimsonamine and galactosamine. Because of its strong effect on Staphylococcus aureus, Escherichia coli, Bacillus aerogenes, Proteus mirabilis, Pseudomonas aeruginosa and other G-bacteria, it is suitable for neonatal sepsis, septicemia, and central nervous system infection caused by sensitive bacteria ( Including meningitis), urinary tract infection, respiratory tract infection, gastrointestinal infection (including peritonitis), biliary tract infection, skin, bone, otitis media, sinusitis, soft tissue infection (including burns), listeriosis, etc., in clinical wide...

Claims

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Application Information

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IPC IPC(8): C07H15/236C07H1/06
CPCC07H15/236C07H1/06
Inventor 王婷
Owner HEBEI SHENGXUE DACHENG PHARMA
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