Method and detection kit used for detecting virus

A virus and magnetic sphere technology, applied in the field of virology and chemical biology, can solve the problems of long time consumption and complicated detection steps, and achieve the effects of high sensitivity, strong specificity and simple separation operation.

Inactive Publication Date: 2012-05-02
WUHAN UNIV
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Problems solved by technology

[0003] The purpose of the present invention is to provide a method for detecting viruses, s

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  • Method and detection kit used for detecting virus
  • Method and detection kit used for detecting virus
  • Method and detection kit used for detecting virus

Examples

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Embodiment 1

[0028] Example 1 Detection of Avian Influenza Virus H9N2

[0029] Taking the avian influenza virus H9N2 as an example, the method for detecting the virus by the fluorescent magnetic immunosandwich method will be described in detail below.

[0030] 1. Method

[0031] 1. Preparation of H9N2 antibody

[0032] (1) Experimental materials

[0033] 1) Immunogen: Avian influenza virus (AIV) H9N2 subtype strain (Institute of Virology, Chinese Academy of Sciences).

[0034] 2) Reagents and media: RPMI-1640 was purchased from GIBCOL, newborn calf serum (super) was purchased from Hangzhou Sijiqing Bioengineering Company; fusion agent 50%PEq was purchased from Sigma; HAT salt (l0()x), GIBCOL; HT salt (100x), GIBCOL; Lymphocyte Separation Solution: Tianjin Zongyang Biological Products Technology Co., Ltd.; Streptomycin is a product of North China Pharmaceutical Factory; Goat anti-mouse IgG-HRp: Huamei Company; Colchicine: Sigma Company; Freund Complete and Incomplete Freund's Adjuva...

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Abstract

The invention relates to a method and a detection kit used for detecting a virus. The method comprises steps that: an antibody of the glycoprotein of a target virus adventitia is coupled with magnetic nano/micro-spheres, such that immunized magnetic nano/micro-spheres are obtained; the immunized magnetic nano/micro-spheres are used for catching the target virus in the sample, such that a magnetic sphere virus composition is obtained; the antibody of the glycoprotein of the adventitia of the biotinylated virus is incubated with the magnetic sphere virus composition; the obtained material is incubated with streptavidin-coupled quantum dots, such that a magnetic sphere-virus-quantum dot composition is obtained; and the virus is detected through the detection upon fluorescence signals of the magnetic sphere-virus-quantum dot composition. With the method, specific recognition, preconcentration, separation, and high-sensitivity detection of H9N2 subtype avian influenza active virus can be realized. The invention also provides a detection kit employed by the method. The kit and the method provided by the invention are characterized by simple operation, high specificity, high sensitivity, and the like. The whole detection process can be finished in one hour.

Description

technical field [0001] The invention relates to a virus detection method, and also relates to a detection kit used in the method, belonging to the fields of virology and chemical biology. Background technique [0002] The new technology of rapid detection of pathogens with high sensitivity and specificity is of great importance to food safety, disease prevention and treatment, effective diagnosis and treatment, and national life safety in the world (my country). With the outbreak of infectious diseases such as SARS, AIDS, and H1N1 influenza virus, it is urgent to develop new pathogen detection methods to effectively control the epidemic of infectious diseases. Avian influenza virus has received more and more attention because of its high infectivity, susceptibility to pathogenicity and harm to humans and poultry. For example, the current detection methods of avian influenza virus mainly include nucleic acid detection, chicken embryo pathogen isolation and so on. However, t...

Claims

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Application Information

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IPC IPC(8): G01N33/577G01N21/64
Inventor 庞代文赵蔚张万坡张志凌王汉中
Owner WUHAN UNIV
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