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Method for preparing theaflavin by enzyme catalysis and product prepared by method

A technology for catalytic preparation and theaflavin, applied in the field of tea processing, can solve the problems of high cost of immobilized enzymes, changes in the expression of steric phase activity, etc., and achieve the effects of high activity and content of theaflavin, little impact, and simple processing

Pending Publication Date: 2021-03-26
南通德诺生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The cost of immobilized enzymes is high, and the expression of the enzyme's spatial phase activity will change after immobilization

Method used

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  • Method for preparing theaflavin by enzyme catalysis and product prepared by method
  • Method for preparing theaflavin by enzyme catalysis and product prepared by method
  • Method for preparing theaflavin by enzyme catalysis and product prepared by method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0092] Preparation of leaf powder: 100 kilograms of freshly plucked fresh tea leaves (picked in August in Mashan, Wuxi, Jiangsu), shredded and crushed after withering, put into a temperature-controlled stainless steel extraction kettle with a sieve plate at the bottom and a side filter outlet, and added 800 liter of petroleum ether, stirred at room temperature for 60 minutes, filtered off the dark green petroleum ether solution and recovered petroleum ether.

[0093] Aerobic fermentation: continue to add 700 liters of ethyl acetate, 20 kg of green tea water extract with a tea polyphenol content of 35% and 15 liters of water into the extraction kettle, blow in oxygen at room temperature or 0°C-20°C and stir for fermentation. And detect the absorbance change of fermented liquid at 380 nanometers place with ultraviolet spectrophotometer, treat that the A380 absorbance of fermented liquid reaches peak value and begin to walk usually stop fermentation, filter and obtain leaf residue...

Embodiment 2

[0104] Preparation of leaf powder: 100 kilograms of freshly plucked fresh tea leaves (picked in August in Mashan, Wuxi, Jiangsu), shredded and crushed after withering, put into a stainless steel extraction kettle with a sieve plate at the bottom and a side filter outlet, and added 800 liters of Petroleum ether, stirred at room temperature for 60 minutes, filtered off the dark green petroleum ether solution and recovered petroleum ether.

[0105] Aerobic fermentation: continue to add 600 liters of ethyl acetate, 10 kg of low-caffeine green tea extract containing 85% tea polyphenols (caffeine content is less than 1%) and 8 liters of water in the extraction kettle, and blow it in at room temperature or low temperature Oxygen is stirred and fermented, and the absorbance change of the fermented liquid at 380 nanometers is detected by a UV spectrophotometer. When the A380 absorbance of the fermented liquid reaches a peak and starts to walk, the fermentation is stopped, and the orange...

Embodiment 3

[0117] Preparation of leaf powder: 100 kg of freshly plucked fresh tea leaves (picked in August in Mashan, Wuxi, Jiangsu), withered, chopped and pulverized, put into a stainless steel extraction kettle with a sieve plate at the bottom and a side filter outlet, and then added 100 kg Extract green tea powder as the raw material of tea polyphenols, add 800 kg of petroleum ether and 100 kg of water, stir at room temperature for 60 minutes, filter out the dark green petroleum ether solution and recover the petroleum ether. Add 800 liters of sherwood oil again in the extraction kettle, stir at room temperature for 60 minutes, filter off the light green sherwood oil solution and reclaim sherwood oil.

[0118] Aerobic fermentation: continue to add 800 kg of ethyl acetate to the extraction kettle, blow in oxygen at room temperature or at a low temperature of 0°C-20°C and stir for fermentation, and use a UV spectrophotometer to detect the change in the absorbance of the fermentation brot...

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Abstract

The invention discloses a method for preparing theaflavin through enzyme catalysis and a product prepared by the method.The method comprises the steps: step S1, crushing fresh tea leaves, and adding petroleum ether or ethyl acetate and performing uniform stirring to obtain a mixture; step S2, removing the petroleum ether or ethyl acetate in the mixture by filtering to obtain leaf powder; step S3,adding a tea polyphenol product and water into the leaf powder, and adding ethyl acetate or butyl acetate to obtain a mixed system; step S4, carrying out aerobic fermentation on the mixed system; andstep S5, taking fermentation broth, and performing drying to obtain theaflavin solids. The petroleum ether or ethyl acetate is added into the fresh tea powder to remove caffeine, theophylline wax, chlorophyll and non-volatile grease so as to prepare the leaf powder containing a polyphenol oxidase enzyme system, and the tea polyphenol product is catalyzed by taking the leaf powder as an immobilizedenzyme catalyst to ferment to prepare the theaflavin; and the natural cured polyphenol oxidase enzyme system is reserved in the leaf powder, and the influence on the enzyme activity is small.

Description

technical field [0001] The invention relates to the technical field of tea processing, in particular to a method for preparing theaflavins by enzymatic catalysis and a product thereof. Background technique [0002] Theaflavin is the main active ingredient in black tea, which has various excellent pharmacological and health effects such as anti-inflammatory, antibacterial, anti-virus, lowering blood fat, weight loss, prevention of osteoarthritis, and cancer prevention. Because the content of theaflavins in black tea is extremely low, the content in finished black tea is only 0.5%-2%, and the extraction and purification are relatively difficult and costly, so it is known as "tea gold". [0003] A series of research results show that theaflavins are not only an effective free radical scavenger and antioxidant, but also have various physiological functions such as anti-cancer, anti-mutation, antibacterial, anti-virus, improvement and treatment of cardiovascular and cerebrovascul...

Claims

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Application Information

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IPC IPC(8): C12P17/16C07D311/62
CPCC12P17/162C07D311/62
Inventor 杨卫国赵凡伟闵小华金钟周广勇管华扬
Owner 南通德诺生物技术有限公司
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