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Method for separating high purity theaflavin from black tea pigment solution

A high-purity, theaflavin technology, applied in the direction of organic chemistry, can solve the problems of expensive instruments or fillers, small preparation volume, and inability to carry out mass production, etc., to achieve easy desorption, easy operation, and large-scale continuous production. Effect

Active Publication Date: 2013-04-03
杭州英仕利生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these methods all have the disadvantages of small preparation volume, expensive equipment or fillers, etc., and cannot be mass-produced.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Take 1 portion of AB-8 and pack it into a column with a column volume of N (ml). Put the black tea pigment solution on the column, and the column volume is N (ml). With 20%, 30%, 40%, 50%, 60% ethanol each N (ml) for elution, the elution rate is 2BV / h. The eluate was collected and freeze-dried to obtain high-purity theaflavin.

Embodiment 2

[0025] Take 1 portion of AB-8 and pack it into a column with a column volume of N (ml). Put the black tea pigment solution on the column, and the column volume is 0.6N (ml). Elute with 20%, 40%, and 60% ethanol in N (ml), and the elution rate is 3BV / h. The eluate is collected and spray-dried to obtain high-purity theaflavins.

Embodiment 3

[0027] Take 1 portion of NKA-9 and pack it into a column with a volume of N (ml). Put the black tea pigment solution on the column, and the column volume is 0.5N (ml). Elute with 20% and 60% ethanol in N (ml), and the elution rate is 2BV / h. The eluate was collected and freeze-dried to obtain high-purity theaflavin.

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PUM

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Abstract

The invention discloses a method for separating high-purity theaflavin from black tea pigment solution. The black tea pigment solution obtained by extracting black tea or oxidation of tea polyphenols is used as raw material, and AB-8 or NKA-9 or Mitsubishisp-207 and other large Porous resin is used as a separation medium, and ethanol aqueous solution is used as an eluting solution to separate and obtain high-purity theaflavin; the method of the invention has simple process, low energy consumption, simple and safe operation, and is beneficial to realizing large-scale continuous production.

Description

technical field [0001] The invention relates to a biochemical extraction technology, in particular to a method for separating high-purity theaflavin from black tea pigment solution by macroporous resin column chromatography. Background technique [0002] Black tea pigment is the oxidation product of tea polyphenols, which is a natural pigment and is currently used as a food additive and pharmaceutical intermediate. The main components of tea pigment are theaflavin, thearubigin and theabrownin. There are generally several methods for obtaining black tea pigments, such as in vivo oxidation (black tea processing), chemical oxidation and enzymatic oxidation. Fresh tea leaves contain a large amount of polyphenolic compounds, accounting for 18%-36% of their dry weight. The polyphenols in fresh leaves are mainly catechins (flavan-3-ols), which account for about 80% of the total polyphenols. During black tea processing, catechins in fresh tea leaves are oxidized to theaflavins an...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07D311/62
Inventor 屠幼英
Owner 杭州英仕利生物科技有限公司
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