Disinfectant composition and disinfectant for disinfecting hatching eggs and application of disinfectant composition and disinfectant
By using a combination of disinfectant of permanganate inorganic compounds and quaternary ammonium organic compounds, the problem of incomplete disinfection and sterilization of seed eggs is solved, and efficient seed egg disinfection is achieved, ensuring the hatching rate and the integrity of the eggshell membrane.
Patent Information
- Application Number
- CN202411704149.8
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2024-11-26
- Publication Date
- 2025-03-11
AI Technical Summary
The existing breeding egg disinfection methods have problems such as incomplete sterilization, harmful to experimental personnel, and damage to the egg shell membrane, resulting in a decrease in hatching rate.
A combination of disinfectants is used, including permanganate inorganic compounds and quaternary ammonium organic compounds, with a weight ratio of 1: (1-2), and is used to prepare a disinfectant and disinfection is achieved by soaking seed eggs.
On the premise of ensuring that the eggshell membrane is not damaged, the mortality rate of bacteria and pathogens on the surface of the breeding egg is significantly improved, the hatching rate is increased, and the harm to the experimenter is reduced.
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Figure CN119586627A_ABST
Abstract
Description
Technical Field
[0001] The present application relates to the technical field related to disinfection of breeding eggs, and specifically provides a disinfectant combination and disinfectant solution for disinfection of breeding eggs and uses thereof. Background Art
[0002] Breeding eggs need to be disinfected and sterilized before hatching. If the surface of the breeding eggs is not completely sterilized, after the breeding eggs with bacteria and pathogens on the surface enter the incubator, during the incubation process, bacteria and other pathogens may enter the breeding eggs through the pores, contaminate the breeding eggs, reduce the hatching rate, and affect the progress of the experiment.
[0003] At present, the disinfection methods of breeding eggs are usually divided into chemical disinfection and physical disinfection. The physical disinfection method mainly uses pasteurization, ultraviolet sterilization, ozone and negative ion sterilization for disinfection; the chemical disinfection method mainly includes fumigation disinfection and immersion disinfection. Among them, the fumigation disinfection method requires a relatively closed place. For small-scale breeding egg incubation in the laboratory, due to insufficient laboratory conditions, the sterilization of breeding eggs is incomplete and harmful to the experimenters. For the immersion disinfection method, if the concentration of the disinfectant in the disinfectant is low, it has a lower sterilization rate for breeding eggs; if the concentration of the disinfectant in the disinfectant is high, although it has a better sterilization rate, it is easy to damage the eggshell membrane, which greatly reduces the hatching rate of breeding eggs. Summary of the invention
[0004] In view of this, the present application provides a disinfectant combination and disinfectant solution for disinfecting breeding eggs and their uses. The disinfectant combination used in the present application improves the mortality rate of bacteria and pathogens on the surface of breeding eggs while ensuring that the eggshell membrane is not damaged.
[0005] In a first aspect, the present application provides a disinfectant combination for disinfecting hatching eggs, the disinfectant combination comprising a first disinfectant and a second disinfectant; the first disinfectant comprises a permanganate inorganic compound; the second disinfectant comprises a quaternary ammonium salt organic compound; the weight ratio of the permanganate inorganic compound to the quaternary ammonium salt organic compound is 1:(1-2) (e.g., 1:1.1, 1:1.2, 1:1.3, 1:1.4, 1:1.5, 1:1.6, 1:1.7, 1:1.8, 1:1.9 or 1:2, etc.);
[0006] The quaternary ammonium salt organic compound comprises a compound represented by formula (I) and a compound represented by formula (II) in a weight ratio of 1:(0.25-4);
[0007]
[0008] In formula (I), X' is selected from Cl or Br;
[0009] R1' and R2' are each independently selected from C1-C3 alkyl;
[0010] R3' and R4' are selected from C8-C12 alkyl groups, and R3' and R4' are the same;
[0011]
[0012] In formula (II), X" is selected from Cl or Br;
[0013] R1" and R2" are each independently selected from C1-C3 alkyl;
[0014] R3" is selected from C6-C10 alkyl, R4" is selected from C8-C12 alkyl, and R3" and R4" are different.
[0015] In some optional embodiments, R1' and R2' are each independently selected from C1-C3 straight chain alkyl groups.
[0016] In some alternative embodiments, R1' and R2' are each independently selected from methyl, ethyl or propyl. In some specific embodiments, R1' and R2' are both selected from methyl.
[0017] In some optional embodiments, R3' and R4' are selected from C8-C12 straight chain alkyl (eg, C8-C11 straight chain alkyl, C8-C10 straight chain alkyl, C9-C12 straight chain alkyl, C9-C11 straight chain alkyl, C9-C10 straight chain alkyl or C10 straight chain alkyl, etc.), and R3' and R4' are the same.
[0018] In some alternative embodiments, R3' and R4' are selected from octyl, nonyl, quinyl, undecyl or dodecyl, and R3' and R4' are the same.
[0019] In some embodiments, R3' and R4' are both selected from quinyl.
[0020] In some optional embodiments, the compound represented by formula (I) is selected from at least one of bis-octyl dimethyl ammonium chloride (CAS No. 5538-94-3), bis-octyl dimethyl ammonium bromide, bis-nonyl dimethyl ammonium chloride, bis-nonyl dimethyl ammonium bromide, didecyl dimethyl ammonium chloride (CAS No. 7173-51-5), didecyl dimethyl ammonium bromide (CAS No. 2390-68-3), didodecyl dimethyl ammonium chloride (CAS No. 3401-74-9) and didodecyl dimethyl ammonium bromide (CAS No. 3282-73-3).
[0021] In some specific embodiments, the compound represented by formula (I) is selected from didecyldimethylammonium chloride.
[0022] In some optional embodiments, R1″ and R2″ are each independently selected from C1-C3 straight chain alkyl;
[0023] In some alternative embodiments, R1" and R2" are each independently selected from methyl, ethyl or propyl.
[0024] In some embodiments, R1" and R2" are both selected from methyl.
[0025] In some optional embodiments, R3" is selected from C6-C10 straight chain alkyl (e.g., C6-C9 straight chain alkyl, C6-C8 straight chain alkyl, C7-C10 straight chain alkyl, C7-C9 straight chain alkyl, C7-C8 straight chain alkyl or C8 straight chain alkyl, etc.), R4" is selected from C8-C12 straight chain alkyl (e.g., C8-C11 straight chain alkyl, C8-C10 straight chain alkyl, C9-C12 straight chain alkyl, C9-C11 straight chain alkyl, C9-C10 straight chain alkyl or C10 straight chain alkyl, etc.), and R3" and R4" are different
[0026] In some optional embodiments, R3" is selected from hexyl, heptyl, octyl, nonyl or quinyl, R4" is selected from octyl, nonyl, quinyl, undecyl or dodecyl, and R3" and R4" are different.
[0027] In some embodiments, R3" is selected from octyl, and R4" is selected from quinyl.
[0028] In some optional embodiments, the compound represented by formula (II) is selected from at least one of octyldecyldimethylammonium chloride (CAS No. 32426-11-2) and octyldodecyldimethylammonium chloride (CAS No. 10361-16-7).
[0029] In some specific embodiments, the compound represented by formula (II) is selected from octyldecyldimethylammonium chloride.
[0030] In some optional embodiments, the permanganate inorganic compound is selected from at least one of lithium permanganate, sodium permanganate, potassium permanganate, ammonium permanganate, calcium permanganate, barium permanganate, zinc permanganate and magnesium permanganate.
[0031] In some specific embodiments, the permanganate inorganic compound is selected from potassium permanganate.
[0032] In some optional embodiments, the use concentration of the inorganic permanganate compound is (50-100) mg / L (for example, 50 mg / L, 55 mg / L, 60 mg / L, 65 mg / L, 70 mg / L, 75 mg / L, 80 mg / L, 85 mg / L, 90 mg / L, 95 mg / L or 100 mg / L, etc.), and the use concentration of the inorganic permanganate compound and the use concentration of the quaternary ammonium salt organic compound is 1:(1-2) (for example, 1:1.1, 1:1.2, 1:1.3, 1:1.4, 1:1.5, 1:1.6, 1:1.7, 1:1.8, 1:1.9 or 1:2, etc.).
[0033] In some specific embodiments, the concentration of the permanganate inorganic compound is 75 mg / L.
[0034] In some optional embodiments, the quaternary ammonium salt organic compound is used in a concentration of (50-200) mg / L; for example, 50 mg / L, 55 mg / L, 60 mg / L, 65 mg / L, 70 mg / L, 75 mg / L, 80 mg / L, 85 mg / L, 90 mg / L, 95 mg / L, 100 mg / L, 105 mg / L, 110 mg / L, 115 mg / L, 120 mg / L, 125 mg / L, 130 mg / L, 135 mg / L, 140 mg / L, 145 mg / L, 150 mg / L, 155 mg / L, 160 mg / L, 165 mg / L, 170 mg / L, 175 mg / L, 180 mg / L, 185 mg / L, 190 mg / L, 195 mg / L or 200 mg / L. In other optional embodiments, the quaternary ammonium salt organic compound is used at a concentration of (75-100) mg / L.
[0035] In some optional embodiments, the concentration of the permanganate inorganic compound is 75 mg / L, and the concentration of the quaternary ammonium salt organic compound is (75-150) mg / L. In some specific embodiments, the concentration of the permanganate inorganic compound is 75 mg / L, and the concentration of the quaternary ammonium salt organic compound is 100 mg / L.
[0036] In a second aspect, the present application provides a disinfectant, which includes the disinfectant combination described in the first aspect.
[0037] In some optional embodiments, the concentration of the permanganate inorganic compound in the disinfectant is (50-100) mg / L (for example, 50 mg / L, 55 mg / L, 60 mg / L, 65 mg / L, 70 mg / L, 75 mg / L, 80 mg / L, 85 mg / L, 90 mg / L, 95 mg / L or 100 mg / L, etc.); and the ratio of the concentration of the permanganate inorganic compound to the concentration of the quaternary ammonium salt organic compound in the disinfectant is 1:(1-2) (for example, 1:1.1, 1:1.2, 1:1.3, 1:1.4, 1:1.5, 1:1.6, 1:1.7, 1:1.8, 1:1.9 or 1:2, etc.).
[0038] In some specific embodiments, the concentration of the permanganate inorganic compound in the disinfectant is 75 mg / L.
[0039] In some optional embodiments, the concentration of the quaternary ammonium salt organic compound in the disinfectant is (50-200) mg / L; for example, 50 mg / L, 55 mg / L, 60 mg / L, 65 mg / L, 70 mg / L, 75 mg / L, 80 mg / L, 85 mg / L, 90 mg / L, 95 mg / L, 100 mg / L, 105 mg / L, 110 mg / L, 115 mg / L, 120 mg / L, 125 mg / L, 130 mg / L, 135 mg / L, 140 mg / L, 145 mg / L, 150 mg / L, 155 mg / L, 160 mg / L, 165 mg / L, 170 mg / L, 175 mg / L, 180 mg / L, 185 mg / L, 190 mg / L, 195 mg / L or 200 mg / L. In other optional embodiments, the concentration of the quaternary ammonium salt organic compound in the disinfectant is (75-100) mg / L.
[0040] In some optional embodiments, the concentration of the permanganate inorganic compound in the disinfectant is 75 mg / L, and the concentration of the quaternary ammonium salt organic compound in the disinfectant is (75-150) mg / L. In some specific embodiments, the concentration of the permanganate inorganic compound in the disinfectant is 75 mg / L, and the concentration of the quaternary ammonium salt organic compound in the disinfectant is 100 mg / L.
[0041] In a third aspect, the present application provides the use of any of the following items in the disinfection of hatching eggs:
[0042] (I) the disinfectant combination described in the first aspect; and / or,
[0043] (II) The disinfectant described in the second aspect.
[0044] In a fourth aspect, the present application provides a method for disinfecting hatching eggs, the method comprising the following steps:
[0045] First, wash the surface of the eggs with (30-40)℃ water; then, soak the eggs in any of the following for (10-30)min; finally, dry the eggs;
[0046] (I), a solution containing the disinfectant combination described in the first aspect; and / or,
[0047] (II) The disinfectant described in the second aspect.
[0048] This application has the following beneficial effects:
[0049] The present application not only has a better bactericidal effect, but also ensures the qualified rate of disinfected breeding eggs, thereby ensuring the hatching rate of breeding eggs, by using a combination of "a compound represented by formula (I) and a compound represented by formula (II)" in a quaternary ammonium salt organic compound and a combination of "a quaternary ammonium salt organic compound and a permanganate inorganic compound" in a disinfectant. DETAILED DESCRIPTION
[0050] The present application discloses a disinfectant combination, a disinfectant solution and their uses for disinfecting breeding eggs. Those skilled in the art can refer to the content of this article and appropriately improve the process parameters to achieve the purpose. It is particularly important to point out that all similar substitutions and modifications are obvious to those skilled in the art and are deemed to be included in this application. The methods and applications of this application have been described through preferred embodiments, and relevant personnel can obviously modify or appropriately change and combine the methods and applications described herein without departing from the content, spirit and scope of this application to implement and apply the technology of this application.
[0051] Unless otherwise specified, all reagents in this application were purchased from the market and were of analytical grade.
[0052] In order to make the purpose, technical solutions and advantages of the present application clearer, the implementation scheme of the present application will be further described in detail below in conjunction with examples.
[0053] Preparation of disinfectant:
[0054] The active ingredients and their concentrations in the disinfectants of Examples 1 to 10 and Comparative Examples 1 to 7 are shown in Table 1.
[0055] Table 1 Active ingredients and their concentrations in the disinfectants of Examples 1 to 10 and Comparative Examples 1 to 7:
[0056]
[0057] The preparation methods of the disinfectants of Examples 1 to 10 and Comparative Examples 1 to 5 specifically include the following steps:
[0058] Step S101, dissolving a permanganate inorganic compound (specifically potassium permanganate) in deionized water to obtain an aqueous solution of the permanganate inorganic compound;
[0059] Step S102, dissolving a quaternary ammonium salt organic compound (specifically didecyl dimethyl ammonium chloride and / or octyl decyl dimethyl ammonium chloride) in deionized water to obtain an aqueous solution of the quaternary ammonium salt organic compound;
[0060] Step S103, mixing the aqueous solution of the permanganate inorganic compound obtained in step S101 and the aqueous solution of the quaternary ammonium salt organic compound obtained in step S102 at a volume ratio of 1:1 to obtain the disinfectant.
[0061] The preparation method of the disinfectant in Comparative Example 6 specifically includes the following steps: dissolving an inorganic permanganate compound (specifically potassium permanganate) in deionized water to obtain an aqueous solution of the inorganic permanganate compound (i.e., the disinfectant in Comparative Example 6).
[0062] The preparation method of the disinfectant in Comparative Example 7 specifically includes the following steps: dissolving a quaternary ammonium salt organic compound (specifically didecyl dimethyl ammonium chloride and octyl decyl dimethyl ammonium chloride) in deionized water to obtain an aqueous solution of the quaternary ammonium salt organic compound (i.e., the disinfectant in Comparative Example 7).
[0063] Application of disinfectant in disinfection of hatching eggs:
[0064] The disinfectants of Examples 1 to 10 and Comparative Examples 1 to 7 were used in the disinfection of breeding eggs. The specific process of breeding egg disinfection is as follows: after receiving 0-day-old breeding eggs (specifically chicken eggs) in the laboratory; first, wash the surface of the breeding eggs with (30-40)°C (specifically 35°C) water; then, immerse the breeding eggs in the disinfectant for (10-30) minutes (specifically 20 minutes); finally, take the breeding eggs out of the disinfectant, wipe them with cotton cloth and dry them.
[0065] Among them, after the disinfectants of Examples 1 to 10 and Comparative Examples 1 to 7 were used to disinfect 1 day-old breeding eggs respectively, the bacterial state on the surface of the breeding eggs was observed under a scanning electron microscope and the mortality rate of the bacteria was calculated; the observation results are shown in Table 2.
[0066] Table 2 Observation results of the disinfectants of Examples 1 to 10 and Comparative Examples 1 to 7 used in the disinfection of breeding eggs:
[0067]
[0068]
[0069] It can be seen from Table 2 that the disinfectant of the present application has a better bactericidal effect.
[0070] By comparing Examples 1 to 4 and Comparative Examples 1 to 3, it can be seen that as the weight ratio of the inorganic permanganate compound and the quaternary ammonium salt organic compound increases, the bactericidal effect of the disinfectant "increases first and then decreases"; and when the weight ratio of the inorganic permanganate compound and the quaternary ammonium salt organic compound is within the range of 1: (1 to 2), the disinfectant has a better bactericidal effect. Further, by comparing Example 2 and Comparative Examples 6 to 7, it can be seen that the disinfectant can significantly improve the bactericidal effect of the disinfectant by using a combination of "inorganic permanganate compounds and quaternary ammonium salt organic compounds". By comparing Examples 2, 5 to 10, it can be seen that as the weight ratio of the compound represented by formula (I) and the compound represented by formula (II) in the quaternary ammonium salt organic compound increases, the bactericidal effect of the disinfectant "increases first and then decreases"; and when the weight ratio of the compound represented by formula (I) and the compound represented by formula (II) is within the range of 1: (0.25 to 4), the disinfectant has a better bactericidal effect. Furthermore, by comparing Example 2 with Comparative Examples 4 to 5, it can be seen that the bactericidal effect of the disinfectant can be significantly improved by using a combination of "a compound represented by formula (I) and a compound represented by formula (II)" in the quaternary ammonium salt organic compound.
[0071] It can be seen from this that the present application can significantly improve the bactericidal effect of the disinfectant by using a combination of "a compound represented by formula (I) and a compound represented by formula (II)" in the quaternary ammonium salt organic compound, and by using a combination of "a quaternary ammonium salt organic compound and a permanganate inorganic compound" in the disinfectant.
[0072] After the disinfectants of Example 2 and Comparative Examples 6-7 were used to disinfect 100 0-day-old breeding eggs received in the laboratory, a scanning electron microscope was used to observe whether the eggshell membranes of the 100 0-day-old breeding eggs were damaged. If the eggshell membrane was damaged, the breeding egg was unqualified, and if the eggshell membrane was not damaged, the breeding egg was qualified. Then, the qualified number of the 100 0-day-old breeding eggs was counted and the qualified rate was calculated. The observation results are shown in Table 3.
[0073] Table 3 Observation results of disinfectant / disinfectant solutions of Example 2 and Comparative Examples 6-7:
[0074] Disinfectants Number of qualified Pass rate Example 2 92 92％ Comparative Example 6 67 67％ Comparative Example 7 79 79％
[0075] It can be seen from Table 2 and Table 3 that the present application not only has a better bactericidal effect by using a combination of "a compound represented by formula (I) and a compound represented by formula (II)" in the quaternary ammonium salt organic compound, and by using a combination of "a quaternary ammonium salt organic compound and a permanganate inorganic compound" in the disinfectant, but also the qualified rate of the disinfected breeding eggs can be guaranteed to ensure the hatching rate of the breeding eggs.
[0076] The above is a detailed introduction to a disinfectant combination, disinfectant solution and their uses for disinfecting hatching eggs provided by the present application. This article uses specific examples to illustrate the principles and implementation methods of the present application. The description of the above embodiments is only used to help understand the method and core idea of the present application. It should be pointed out that for those skilled in the art, without departing from the principles of the present application, several improvements and modifications can be made to the present application, and these improvements and modifications also fall within the scope of protection of the claims of the present application.
Claims
1. A disinfectant combination for disinfecting hatching eggs, characterized in that: The disinfectant combination comprises a first disinfectant and a second disinfectant; the first disinfectant comprises a permanganate inorganic compound; the second disinfectant comprises a quaternary ammonium salt organic compound; the weight ratio of the permanganate inorganic compound to the quaternary ammonium salt organic compound is 1:(1-2); The quaternary ammonium salt organic compound comprises a compound represented by formula (I) and a compound represented by formula (II) in a weight ratio of 1:(0.25-4); In formula (I), X' is selected from Cl or Br; R1' and R2' are each independently selected from C1-C3 alkyl; R3' and R4' are selected from C8-C12 alkyl groups, and R3' and R4' are the same; In formula (II), X" is selected from Cl or Br; R1" and R2" are each independently selected from C1-C3 alkyl; R3" is selected from C6-C10 alkyl, R4" is selected from C8-C12 alkyl, and R3" and R4" are different.
2. The disinfectant combination according to claim 1, characterized in that: R1' and R2' are each independently selected from C1-C3 straight chain alkyl; Optionally, R1' and R2' are each independently selected from methyl, ethyl or propyl; Optionally, R1' and R2' are both selected from methyl.
3. The disinfectant combination according to claim 1, characterized in that: R3' and R4' are selected from C8-C12 straight chain alkyl groups, and R3' and R4' are the same; Optionally, R3' and R4' are selected from octyl, nonyl, quinyl, undecyl or dodecyl, and R3' and R4' are the same.
4. The disinfectant combination according to any one of claims 1 to 3, characterized in that: The compound represented by formula (I) is at least one selected from bis-octyl dimethyl ammonium chloride, bis-octyl dimethyl ammonium bromide, bis-nonyl dimethyl ammonium chloride, bis-nonyl dimethyl ammonium bromide, didecyl dimethyl ammonium chloride, didecyl dimethyl ammonium bromide, didodecyl dimethyl ammonium chloride and didodecyl dimethyl ammonium bromide.
5. The disinfectant combination according to claim 1, characterized in that: R1" and R2" are each independently selected from C1-C3 straight chain alkyl; Optionally, R1" and R2" are each independently selected from methyl, ethyl or propyl. Optionally, R1" and R2" are both selected from methyl.
6. The disinfectant combination according to claim 1, characterized in that: R3" is selected from C6-C10 straight chain alkyl, R4" is selected from C8-C12 straight chain alkyl, and R3" and R4" are different Optionally, R3" is selected from hexyl, heptyl, octyl, nonyl or quinyl, R4" is selected from octyl, nonyl, quinyl, undecyl or dodecyl, and R3" and R4" are different.
7. The disinfectant combination according to claim 1, 5 or 6, characterized in that: The compound represented by formula (II) is at least one selected from octyldecyl dimethyl ammonium chloride (CAS No. 32426-11-2) and octyldodecyl dimethyl ammonium chloride (CAS No. 10361-16-7).
8. The disinfectant combination according to claim 1, characterized in that: The permanganate inorganic compound is selected from at least one of lithium permanganate, sodium permanganate, potassium permanganate, ammonium permanganate, calcium permanganate, barium permanganate, zinc permanganate and magnesium permanganate; Optionally, the permanganate inorganic compound is selected from potassium permanganate.
9. The disinfectant combination according to any one of claims 1 to 8, characterized in that: The concentration of the permanganate inorganic compound is (50-100) mg / L, and the ratio of the concentration of the permanganate inorganic compound to the concentration of the quaternary ammonium salt organic compound is 1:(1-2); Optionally, the concentration of the permanganate inorganic compound is 75 mg / L, and the concentration of the quaternary ammonium salt organic compound is (75-150) mg / L.
10. A disinfectant, characterized in that: The disinfectant solution comprises the disinfectant combination according to any one of claims 1 to 9.
11. Any of the following is used in the disinfection of hatching eggs: (I), the disinfectant combination according to any one of claims 1 to 9; and / or, (II) The disinfectant according to claim 10.
12. A method for disinfecting breeding eggs, characterized in that: The disinfection method comprises the following steps: First, wash the surface of the eggs with (30-40)℃ water; then, immerse the eggs in any of the following for (10-30) minutes; finally, dry the eggs; (I) a solution containing the disinfectant combination described in the first aspect; and / or, (II) the disinfectant solution described in the second aspect.