Method for separating agricus bisporus polysaccharides from agricus bisporus and determining method thereof

Abstract

The invention relates to a method for separating agricus bisporus polysaccharides from agricus bisporus, which comprises the following steps: carrying out vacuum freeze-drying on the agricus bisporus for 24h; extracting crude polysaccharides with hot water: adding distilled water, boiling with big fire, maintaining with slow fire, centrifuging, taking supernatant liquid, precipitating, placing back into a heating device, adding water for continuous heating till boiling, maintaining with the slow fire, re-centrifuging and taking the supernatant liquid; repeating for three times, and using the distilled water for fixing volume of the obtained supernatant liquid for standby; carrying out dual-water phase extraction operation: precisely weighing 2.66g of polyethylene glycol (PEG) 6000 and 4.28g of ammonium sulfate, adding into a 50mL centrifuge tube, adding 20mL of sample diluent, and evenly mixing; simultaneously weighing 2.66g of PEG 6000 and 4.28g of ammonium sulfate, adding into the 50mL centrifuge tube, adding 20mL of distilled water, evenly mixing, and obtaining the agricus bisporus polysaccharides distributed in the PEG phase. The utilization of a dual-water phase system for extracting the agricus bisporus polysaccharides can realize the maximum yield of 80.1%, and the yield can achieve 17.45mg / g.

Description

technical field [0001] The invention belongs to the extraction of biological polysaccharides, in particular to a method for separating Agaricus bisporus polysaccharide from Agaricus bisporus and a determination method thereof. Background technique [0002] At present, the commonly used methods for extracting polysaccharides from edible fungi include: hot water extraction, microwave-assisted method, ultrasonic-assisted method and acid-base extraction method, all of which have certain shortcomings. Among them, the hot water extraction method requires a temperature of 80-100°C and a treatment time of 60-180 minutes. The treatment temperature is relatively high and the time is relatively long, which is easy to denature and inactivate polysaccharides; Permeability and reflectivity enable polar substances, such as water, to be selectively absorbed and heated to increase the permeability of the material, so that active substances such as polysaccharides are released from the tissue...

AI Technical Summary

Problems solved by technology

Among them, the hot water extraction method requires a temperature of 80-100°C and a treatment time of 60-180 minutes. The treatment temperature is relatively high and the time is relatively long, which is easy to denature and inactivate polysaccharides; Permeability and reflectivity enable polar substances, such as water, to be selectively absorbed and heated to increase the permeability of the material, so that active substances such as polysaccharides are released from the tissue. The essence is to extract polysaccharides by heating, so it is easy to make polysaccharides Denaturation and inactivation occur; the ultrasonic-assisted method utilizes the cavitation effect generated in the solvent to cause the formation, growth and explosion compression of cavities in the solution, thereby breaking the solid sample and increasing the contact area between the sample and the extractant , to increase the mass transfer rate of the target product from the solid phase to the liquid phase, the essence is to break the cell wall and dissolve the target product, but in the process of actual operation, the efficiency of using ultrasonic waves to break the cell wall of edible fungi is very low, resulting in the extraction rate of polysaccharides It is also relatively low; although the acid-base extraction method can shorten the extraction time, because the acid and alkali are difficult to control the concentration factor, it is easy to hydrolyze some polysaccharides, thereby destroying the active structure of polysaccharides and reducing the yield of polysaccharides

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