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Pseudomonas syringae and application thereof in prevention of tomato gray mold

A tomato gray mold, pseudomonas technology, applied in the direction of application, bacteria, fungicides, etc., can solve the problem of unsatisfactory cure effect

Inactive Publication Date: 2014-01-22
NORTHEAST AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In terms of medicine, we mainly use vanmycin powder, chlorothalonil smoke shed or metalaxyl wettable powder, but no matter which method is used, the healing effect is not ideal.

Method used

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  • Pseudomonas syringae and application thereof in prevention of tomato gray mold
  • Pseudomonas syringae and application thereof in prevention of tomato gray mold
  • Pseudomonas syringae and application thereof in prevention of tomato gray mold

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Example 1 Determination of growth curve of Pseudomonas syringae ZY-1 strain

[0029] The Pseudomonas syringae ZY-1 strain was inserted into the seed medium at a mass fraction of 5% at 32 °C ± 1 °C, and cultivated under the conditions of 110 r / min. The uninoculated medium was used as a reference. Determination of OD at different times with UV spectrophotometer 600 value to OD 600The value is the ordinate, and the culture time is the abscissa to draw the strain growth curve. It can be seen that at 0-5h, the strain is in the growth lag phase and grows slowly; 5-10h belongs to the logarithmic phase, and the cells grow exponentially and grows faster; 11-15h belongs to the stable phase, with stable and maximum viable bacteria. ; After 16h, it belongs to the decay period, and the amount of viable bacteria decreases rapidly. The experimental results are shown in figure 1 .

Embodiment 2

[0030] Example 2: Preparation of fermentation broth of Pseudomonas syringae ZY-1 strain

[0031] (1) Activation culture of strains: The isolated and preserved Pseudomonas syringae ZY-1 strain was transferred to LB solid medium, cultured at 32 °C ± 1 °C, and the activated bacteria were obtained after culturing for 24 hours. For bacterial species, punch holes with a diameter of 4 mm to obtain a bacterial cake for inoculation;

[0032] (2) Fermentation culture: each 250ml conical flask is filled with 100ml of fermentation broth; after configuration, sterilize it at 121°C for 30min, when cooled to 35°C, inoculate the bacteria under aseptic conditions, and shake at 32°C ± 1°C. The rotation speed was 110r / min, and the culture was carried out for 24h. The obtained fermentation broth was concentrated by a rotary evaporator at 60°C for 2 hours, and then stored in a refrigerator at 4°C for later use.

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Abstract

The invention relates to pseudomonas sp. named ZY-1 and a function of the metabolite thereof in curing plant diseases, wherein the microbial collection number of the pseudomonas syringae is CGMCC (China General Microbiological Culture Collection Center) No.7906, the collection date is July 10, 2013, and the collection institution is the CGMCC. The pseudomonas syringae provided by the invention has the advantages of obvious efficacy, short curing time, no environmental pollution, simplicity and convenience in operation and the like.

Description

technical field [0001] The invention relates to a strain of Pseudomonas syringae, in particular to the application of the strain in the prevention and treatment of tomato botrytis, and belongs to the field of biotechnology. Background technique [0002] Tomato Botrytis cinerea is a common and serious fungal disease. In addition to tomato, it can also damage more than 20 crops such as eggplant, pepper, and cucumber. Years with low temperature and continuous rainy weather are seriously harmful. When the disease is serious, the stems and leaves will die, and a large number of rotten flowers and rotten fruits will directly affect the yield. At present, the commonly used control measures are to pick old leaves, increase ventilation, and reduce air humidity. In terms of medicine, the main use of Wanmexiling powder, chlorothalonil smoke remover or metalaxyl wettable powder, but no matter which method is used, the curing effect is not ideal. In order to seek safe and effective con...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/02A01P3/00C12R1/38
Inventor 曲娟娟金羽于敏张春苗黎扣扣
Owner NORTHEAST AGRICULTURAL UNIVERSITY
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