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Erythrochromogenes and use thereof in biological control of diseases

A technology of Streptomyces chromogenes, dark red, applied in the direction of microorganism-based methods, chemicals for biological control, applications, etc.

Inactive Publication Date: 2010-04-28
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the use of Streptomyceserythrochromogenes in the control of Botrytis cinerea has not been reported yet.

Method used

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  • Erythrochromogenes and use thereof in biological control of diseases
  • Erythrochromogenes and use thereof in biological control of diseases
  • Erythrochromogenes and use thereof in biological control of diseases

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1M

[0031] The identification of embodiment 1MO28

[0032] Based on the morphological characteristics, physiological and biochemical characteristics, and 16S rDNA sequence of Streptomyces, it was identified as Streptomyces erythrochromogenes. The specific identification results are as follows:

[0033] 1. Cell morphology

[0034] Strain MO28 grows well on Gao's No. 1 medium, the colony is oval, dark reddish brown, with irregular edges, and the aerial hyphae are white at first, then light yellow-gray after 2 to 3 days, and gray with time. Hyphae without septa and not broken; aerial hyphae are flexible, multi-branched, spore filaments are straight, hooked, with a first spin or a few loose spirals at the top, and spores are oval (see figure 1 ).

[0035] 2. Physiological and biochemical characteristics

[0036] The physiological and biochemical characteristics of strain MO28 are shown in the following three tables (Table 1, 2, 3): MO28 strain can produce H 2 S; gelatin liquefact...

Embodiment 2

[0049] Embodiment 2 antibacterial spectrum analysis

[0050]Plate inhibition detection of pathogenic fungi: The plate confrontation method was used to detect the inhibition of MO28 on pathogenic fungi. Cultivate the MO28 strain LB culture medium at 28°C and 160rpm for 48 hours, then draw 100μl of the bacterial suspension and spread it on the Gaoshi No. 1 medium plate. After the MO28 is full of the culture dish, punch out a 5mm MO28 bacterial cake with a puncher; in the PDA Inoculate the MO28 bacterial cake at a distance of 25 mm from the center of the plate, 2 bacterial cakes per plate, inoculate a 5 mm diameter bacterial cake of the pathogenic fungus to be tested in the center of the plate after culturing at 28°C for 2 days, so that the three bacterial cakes form a straight line. The plate inoculated with target pathogenic bacteria but not biocontrol bacteria was used as a control, and each treatment was repeated 3 times, and cultured at 25°C until the control treatment cover...

Embodiment 3M

[0060] Example 3 MO28 is detected on the control effect of Botrytis cinerea on isolated tomato fruits

[0061] Indoor detection of control effect on tomato fruit botrytis. Select healthy isolated tomato fruits, culture MO28 in PDA medium at 28°C and 160rpm for 96h, then centrifuge at 12000rpm for 10min, and take the supernatant as the supernatant of MO28 fermentation broth; after culturing Botrytis cinerea on the PDA plate for 7d, use sterile Wash the Botrytis cinerea spores with water, and use a hemocytometer to calibrate to 10 6 spores / mL, prepared as a suspension of Botrytis cinerea (B. cinerea) spores. Detection of MO28 on the control effect of Botrytis cinerea on tomato fruits is set as follows: positive control (only inoculated with Botrytis cinerea), negative control (only inoculated with sterile water), supernatant of bacterial strain MO28 fermentation broth + Botrytis cinerea, 28% Botrytis cinerea. Wettable powder 1000 times dilution + Botrytis cinerea. There were ...

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Abstract

The invention provides streptomyces erythrochromogenes MO28, which has a preservation number of CGMCC No.3228. The strain is capable of inhibiting the growth of tomato gray mold. Results of panel antibacterial tests show that MO28 strain has high inhibition effect on various plant pathogenic fungi and plant pathogenic bacteria, wherein the fusarium moniliforme inhibition capacity is the highest and the fusarium moniliforme inhibition rate is up to 69.9 percent; and the pseudomonas syringae inhibition diameter is 53.7millimeter. The tomato fruit gray mold prevention and control efficiency of the MO28 is 96.56 percent and the tomato leaf gray mold prevention and control efficiency of the MO28 is 78.26 percent. The strain has no obvious difference from 28-percent chlorothalonil and diethofencarb wettable powder. Results of greenhouse prevention effect detection show that the efficiency of the prevention and control of gray mold on tomatoes, pepper, cucumbers, egg plants and strawberry of the MO28 is between 63 and 77 percent.

Description

technical field [0001] The invention relates to a new microbial strain and its application, in particular to a Streptomyces strain of Streptomyces chromogenes and its application in biological disease control. Background technique [0002] Botrytis cinerea is a worldwide important disease, which mainly harms fruit and poses a great threat to tomato production. my country began to spread in the 1980s, and it has occurred in various places at present. It has become a restrictive obstacle for tomato protected cultivation. Generally, it can cause a 20% to 30% reduction in tomato production, and it can be as high as more than 50% in severe cases, seriously affecting tomato production and production. quality. Therefore, the control of Botrytis cinerea has become a key measure to protect the development of production, which directly determines the yield and economic benefits of tomato. [0003] Botrytis cinerea Pers. is a fungal disease caused by Botrytis cinerea Pers., which belo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/02A01P3/00A01P1/00C12R1/465
Inventor 吴文良郭岩彬孟凡乔齐琳夏娜焦子伟
Owner CHINA AGRI UNIV
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