Phytophthora-resistant actinomyces and application thereof

A technology for actinomycetes and Phytophthora, applied in the application, bacteria, fungicides and other directions, to achieve the effect of significant inhibition and deepening of understanding

Inactive Publication Date: 2013-10-23
HUNAN AGRICULTURAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Although there are still many problems in biologica

Method used

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  • Phytophthora-resistant actinomyces and application thereof
  • Phytophthora-resistant actinomyces and application thereof
  • Phytophthora-resistant actinomyces and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] The screening of the actinomycetes strain L24 of embodiment one resistance Phytophthora

[0039] The specific process is as follows:

[0040] 1. Collection of soil samples

[0041] About 200g of soil at a depth of 3-10cm from different plots was collected from various parts of Hunan Province, a total of 32 samples were brought back to the laboratory, air-dried naturally, and separated after passing through a 20-mesh sieve.

[0042] 2. Isolation of actinomycetes

[0043] Separation by plate method: Weigh 10g of soil sample, add it to a conical flask filled with 100mL of sterile water, shake it in an oscillator for 30 min, and then prepare 10 -2 、10 -3 、10 -4 、10 --5 Pipette 1 mL of suspensions of different concentrations into sterilized petri dishes, 3 dishes for each concentration, then pour 15-18 mL of Gaoshi No. After being cooled and condensed into a plate, it was placed in an incubator at 30°C for 2-3 days. The single actinomycetes colony with obvious colon...

Embodiment 2

[0051] Taxonomic status of Example 2 Actinomycetes strain L24

[0052] (1) Observation of morphology and culture characteristics

[0053] Using Gao's No. 1 medium and the insert method to inoculate, it was observed under the electron microscope that the spore filaments of the L24 strain first spiraled to a spiral shape, and the spores were long oval and smooth ( Figure 5 , 6 ). Visually observe the color changes of aerial hyphae, basal hyphae and soluble pigments inoculated by streaking on different media plates, and take the color at the mature stage as the basis for species determination (Table 1).

[0054] Table 1 Culture characteristics of L24 strain

[0055] Medium type aerial hyphae base hyphae Soluble pigment Gao Shi No. 1 Newborn off-white, mature gray-brown White none starch medium Newborn white, mature taupe White none PDA Newborn white, mature lavender yellow white none Cha Pepper Agar Newborn off-white, ...

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Abstract

The invention discloses a phytophthora-resistant actinomyces, which belongs to Streptomyces humidus. The phytophthora-resistant actinomyces is preserved in China Center for Type Culture Collection with an accession number of CCTCC No: M2013268. The fermentation broth of the actinomyces provided by the invention has an obvious inhibiting effect on the growth of phytophthora and is applicable to plant diseases caused by phytophthora.

Description

technical field [0001] The invention belongs to the field of agricultural science and technology, specifically relates to the field of biological control, and more specifically relates to Streptomyces hygrophyllum ( Streptomyces humidus ) and their application in biological control. Background technique [0002] potato( Solanum tuberosum L.) is the main food crop in the world, and its annual consumption is second only to rice and wheat [1] It is also a raw material crop for vegetable, feed, processing and industry, and plays an important role in agricultural production and people's life. Late blight is a devastating disease in potato production, which spreads widely in most potato cultivation areas in the world, causing huge losses. The most famous example is the Great Irish Famine caused by late blight in 1845, which directly led to the death of millions of people and the emigration of millions of people. At present, the hazards, difficulty of control and social impac...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/00A01N63/02A01P3/00C12R1/465
Inventor 周倩刘珊珊熊兴耀朱宏建刘双清
Owner HUNAN AGRICULTURAL UNIV
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