Chitosan oligosaccharide copper complex fungicide and application of chitosan oligosaccharide copper complex fungicide

A technology of copper complexes and chitosan oligosaccharides, which is applied in the direction of fungicides, applications, biocides, etc., can solve the problems of safety hazards, drug resistance, etc., and achieve significant control effects, robust growth, and reduced use.

Inactive Publication Date: 2014-07-30
潍坊万胜生物农药有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In China, carbendazim is often used for the prevention and treatment of fungal diseases. The long-term use of the above-mentioned drugs is prone to safety hazards and drug resistance.
And utilize chitosan oligosaccharide copper complex to carry out the control of fungal disease and have not yet been reported

Method used

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  • Chitosan oligosaccharide copper complex fungicide and application of chitosan oligosaccharide copper complex fungicide
  • Chitosan oligosaccharide copper complex fungicide and application of chitosan oligosaccharide copper complex fungicide
  • Chitosan oligosaccharide copper complex fungicide and application of chitosan oligosaccharide copper complex fungicide

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] 1. Production of chitosan oligosaccharide copper complex:

[0020] A. According to different molecular weights, add a certain amount of deionized water to chitosan oligosaccharide, stir evenly, add copper sulfate, dissolve and stir uniformly, form 1% chitosan oligosaccharide copper complex (according to the total mass of chitosan oligosaccharide and copper ion meter) solution. The mass ratio of chitosan and copper ions is 1:1. The above 1% chitosan oligosaccharide copper complex solution was used in Example 2 to verify the indoor effect on plant pathogenic bacteria.

[0021] B. The solution containing a certain amount of copper ions, according to the molecular weight of chitosan oligosaccharides and water solubility, add water to fully stir and dissolve to form a chitosan oligosaccharide copper complex solution. The mass ratio of chitosan and copper ions is 2:1.

[0022] 2. 10% chitosan oligosaccharide copper complex water

[0023] Add chitosan oligosaccharides with...

Embodiment 2

[0028] Embodiment 2 Chitosan oligosaccharide copper complex is to the indoor effect verification of plant pathogenic bacteria

[0029] 1. Experimental method

[0030] Plate inhibition zone method was used.

[0031] 1) Test equipment: aseptic operating table, sterilizing pot, biological incubator, shaking table. PDA medium, sterile water.

[0032] 2) Test method: first, transfer the pathogenic bacteria of Zingiberella gingivalis into the PDA liquid medium, and after culturing for 30 hours, draw 100 microliters onto the PDA plate and spread evenly. Use a hole puncher to punch holes (aperture D=0.6cm) on the culture medium coated with pathogenic bacteria. Then inject 50 microliters of test solutions (1% chitosan oligosaccharide copper complex and 1% copper sulfate solution prepared in Example 1) into the holes respectively.

[0033] They were cultured in a biochemical incubator at 28°C, and each treatment was repeated 4 times. The investigation was started at 24 hours, and t...

Embodiment 3

[0041]Example 3 The verification of the effect of chitosan oligosaccharide copper complex water agent on the control test of cucumber horn spot disease

[0042] Cucumber bacterial angular spot (Pseudomonassyringae pv. lachrymans Smithet Bryan. Yong, Dye & Wilkie) is a bacterial disease. Pseudomonas syringae pathogenic type of cucumber angular spot is one of the important diseases on cucumber, and the pathogen survives the winter in the seeds, outside or with the diseased residues in the soil. The pathogen spreads in the field through irrigation, wind and rain, airflow, insects and agricultural operations, and invades the host through stomata, wounds and water holes. The humidity is high, the disease is severe, and the disease is easy to spread after the storm. Bacterial angular spot produces needle-like water-soaked lesions on the back of the leaves, often dozens of lesions occur at the same time.

[0043] 1. Test materials

[0044] 1. Drugs to be tested

[0045] 10% chito...

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PUM

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Abstract

The invention discloses chitosan oligosaccharide copper complex fungicide and application of the chitosan oligosaccharide copper complex fungicide. An active component of the fungicide is a chitosan oligosaccharide copper complex formed by the complexing of chitosan oligosaccharide and copper ions, wherein the mass ratio of the chitosan oligosaccharide to the copper ions is 5:1-1:1.2, the average molecular weight of the chitosan oligosaccharide is 161-20000, and the degree of deacetylation is greater than or equal to 85%. Experiments show that the chitosan oligosaccharide copper complex fungicide disclosed by the invention is effective in the prevention and treatment of all bacterial diseases of plants, and only can be used for preventing and treating fungal diseases, such as downy mildew, phytophthora disease, powdery mildew and cotton phytophthora disease, of the plants.

Description

technical field [0001] The invention belongs to the application field of plant disease control. Specifically, the present invention relates to a complex fungicide of chitosan oligosaccharide and copper ion and its application. Background technique [0002] There are more than 650 kinds of bacterial plant diseases in 30 genera in the world, and there are more than 150 kinds in my country. Among them, rice bacterial streak disease, rice bacterial blight, rice base rot, citrus canker, pomelo canker, cucumber bacterial angular spot, cotton angular spot, garlic leaf blight, etc. blight, melon angular spot, cabbage soft rot, peanut bacterial wilt, tobacco wildfire, tobacco bacterial wilt, konjac soft rot, ginger blast, pepper bacterial wilt, peach bacterial perforator, melon bacterial wilt Fruit spot, cherry root cancer and bacterial diseases of flower seedlings, etc. Due to the rapid reproduction of bacteria and the lack of control agents, it seriously affects the output and q...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N55/02A01N25/02A01N25/14A01P1/00A01P3/00
Inventor 牛赡光牛兆颖
Owner 潍坊万胜生物农药有限公司
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