Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

High throughput bean quarantine plant pathogenic bacteria detection method and locking type probe

A technology of phytopathogenic bacteria and padlock probes, which is applied in biochemical equipment and methods, measurement/inspection of microorganisms, DNA/RNA fragments, etc., can solve the problems of no detection of padlock probes or related detection methods, and achieve The effect of high sensitivity and strong specificity

Inactive Publication Date: 2015-08-26
SHENZHEN ACAD OF INSPECTION & QUARANTINE +1
View PDF2 Cites 9 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are currently no padlock probes or related assays for the detection of PSPHA and PSPI

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • High throughput bean quarantine plant pathogenic bacteria detection method and locking type probe
  • High throughput bean quarantine plant pathogenic bacteria detection method and locking type probe
  • High throughput bean quarantine plant pathogenic bacteria detection method and locking type probe

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0053] 1. Materials

[0054] 1.1 Test strains

[0055] The strains tested in this example are shown in Table 1.

[0056] Table 1 Conditions of tested strains

[0057] Numbering

Chinese name

strain

470101

Oat acidophilus

Acidovorax avenae subsp. avenae

470122

Bacterial leaf spot of melons

Acidovorax avenae subsp.citrulli

470308

tomato bacterial canker

Clavibacter michiganensis subsp. michiganensis

470326

Corynebacterium michiganii subsp. checkerboard

Clavibacter michiganensis subsp. tessellarius

470356

Bean bacterial wilt

Curtobacterium flaccumfaciens pv.flaccumfaciens

470363

beet bacterial leaf spot

Curtobacterium flaccumfaciens pv.betae

470404

E. amylovora

Erwania amylovora

470550

Peach canker

Pseudomonas syringae pv. persicae

470520

Canker sore kiwifruit

Pseudomonas syringae pv. actinidiae

470522

corn bacterial w...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to a high throughput bean quarantine plant pathogenic bacteria detection method and a locking type probe. According to the detection method, the locking type probe can perform rolling circle amplification simultaneously on pea bacterial phytophthora and kidney bean dizzy phytophthora and then performs high throughput detection on the two kinds of important bean quarantine plant pathogenic bacteria. The detection method is high in specificity and sensitivity, can shorten the detection time of the bean quarantine plant pathogenic bacteria, quicken the pass speed of cargoes imported and exported in China ports, reduces enterprise costs, meanwhile can improve import bean product epidemic situation intercept rate, increase the capability of preventing foreign pest from invading, has important technicality and practicability and can generate obvious economic and social benefits.

Description

technical field [0001] The application relates to the field of detection of bean quarantine plant bacteria, in particular to a high-throughput detection method for bean quarantine plant pathogenic bacteria, and a padlock probe used in the detection method. Background technique [0002] Pseudomonas syringae pv. phaseolicola (PSPHA for short) mainly harms commercial bean crops, causing bacterial sickness of bean and causing serious economic losses. Although many countries have taken preventive measures against bean sickle blight, its spread is alarming, and it is distributed in more than 60 countries on all continents of the world. The fungus is a dangerous organism that is not widely distributed in my country. This disease has only been found in Heilongjiang. At present, the fungus is listed as a quarantine pest for entry into my country. The pathogen is mainly transmitted by seeds, and generally can survive in the seeds for more than 2 years. Once it invades my country, it ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12Q1/68C12Q1/04C12N15/11
CPCC12Q1/689C12Q2531/125
Inventor 冯建军李志锋章桂明王忠文
Owner SHENZHEN ACAD OF INSPECTION & QUARANTINE
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com